LOCUS       sequence-180005-        7263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP1.19.3
FEATURES             Location/Qualifiers
     source          1..7263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2760..2801
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2811..2828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2851..2868)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2857..3081
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3127..3555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3214..3233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3424..3445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3564..3584)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3569..3899
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3750..3885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3812..3831
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3966..4760
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4020..4039)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4630..4649
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4936..5057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4973..4992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5027..5046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5106..5122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5106..5122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5119..5141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5130..5146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5154..5160,5161..5178,5179..5184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5199..5220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5337..5354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5508..6096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5588..5607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6267..7058,7059..7127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6890..6909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7128..7232)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccat gggttgctct ttctctatct
      961 tcctcttggc tttgctgtcc tgtttgacca tcccagcttc cgcttatgag gtgcgcaacg
     1021 tgtccggggt gtaccaagtc acgaacgact gctccaacac aagcattgtg tatgaggcag
     1081 cggacatgat tatgcatatc cccgggtgcg tgccctgtgt tcgggagacc aacttctccc
     1141 gctgctgggt accgctcacc cccacgctcg cggctaggaa cgccagcatc cccactacga
     1201 caatacgacg tcacgtcgat ttgctcgttg gggcggctgc tttctgctcc gctatgtacg
     1261 tgggggatct ctgcggatct gttttccttg tctcccagct gttcaccttc tcgcctcgcc
     1321 gacatgagac agtacaggac tgcaattgct caatctatcc cggccacgta tcaggccatc
     1381 gcatggcttg ggatatgatg atgaactggt ctcctacaac agccttagtg gtatcacagt
     1441 tactccggat cccacaagct gtcgtggaca tggtggcggg cgcccactgg ggaatcctgg
     1501 cgggcctcgc ctactattcc atggcgggga actgggctaa ggtcttaatt gtgatgctac
     1561 tctttgccgg cgttgacggg accgggacct acacgacggg gggggcccag ggccgcaacg
     1621 cccatcggct tacgtccttc ttttcatttg gggcatctca gaatatccag ctcataaaca
     1681 ccaacggcag ctggcatatc aacaggactg ctttgaactg caacgactcc ctcaacactg
     1741 gtttcatcgc cgcgctgttc tacacacaca aattcaacgc gtccggatgc ccggagcgca
     1801 tggccagctg tcgccccatt agcgagttcg ctcaggggtg gggtcccatc acttatgccg
     1861 agtcgaaaag ctcggaccag aggccctatt gttggcacta cgctcctgaa ccgtgcggta
     1921 tcgtggccgc gtcgcaggtg tgtggtccag tatactgctt caccccaagc cccgttgtgg
     1981 tggggacgac cgatcgcttc ggcgtcccta cgtacacctg gggggagaat gagacggacg
     2041 tgctgcttct caacaacacg cggccgccgc aaggcaactg gttcggctgt acgtggatga
     2101 atagcactgg gttcaccaag acgtgtgggg gccccccgtg caacatcggg ggggttggca
     2161 acaacaccct gacctgtccc acggactgtt tccggaagca ccccgaggcc acctacacca
     2221 aatgcggttc agggccttgg ctcacaccta ggtgcctggt tgactaccca tacaggcttt
     2281 ggcactaccc ctgcaccgtc aactttacca tcttcaaagt taggatgtat gtggggggcg
     2341 tggagcatag gctcaacgcc gcatgcaatt ggacccgagg agagcggtgc aacttggaag
     2401 acagggatag atcagagctc agcccgctgc tgctgtctac gacagagtgg cagatattgc
     2461 cttgttcctt caccaccttg ccggctctgt ccactggttt gatccatctc caccagaaca
     2521 tcgtggacgt gcaatacctg tatggtatgg ggtcggcggc cgtctccttt gcaatcaaat
     2581 gggagtatgt cctgttgctc ttccttctcc tggcggacgc gcgcgtttgt gcctgcttgt
     2641 ggatgatgct gctgatagcc caggctgagg cctaatctag aaactttaag ggtcaagaca
     2701 attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag
     2761 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
     2821 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     2881 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2941 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3001 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3061 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     3121 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3181 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3241 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc
     3301 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3361 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3421 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3481 atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3541 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3601 ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3661 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3721 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3781 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3841 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3901 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     3961 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4021 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4081 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4141 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4201 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4261 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4321 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4381 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4441 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     4501 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4561 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4621 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4681 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4741 ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg
     4801 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4861 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4921 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4981 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5041 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     5101 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5161 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5221 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5281 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5341 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5401 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5461 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5521 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5581 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5641 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5701 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5761 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5821 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5881 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5941 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6001 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6061 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6781 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7201 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7261 gtc
//
