LOCUS       sequence-180025-        6827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP2.4.1
FEATURES             Location/Qualifiers
     source          1..6827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             704..712
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(1843..1860)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1849..2073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2205..2793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2285..2304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3122..3169
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3194..3213
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3401..4189)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3512..3531)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4116..4135
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4224..4581)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4238..4373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4390..4581
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4538..4558
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4641..5432,5433..5501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5264..5283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5502..5606)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5633..6088
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5720..5739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5930..5951
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6100..6479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6480..6683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6634..6654
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6728..6747
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6728..6746
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctcgagcca tgggttgctc tttttctatc ttcttacttg cccttctgtc gtgccttaca
       61 gtgccagcgt ctgcggtgga agttaggaac gtcagttcta gctactatgc caccaatgat
      121 tgctcgaaca gcagcatcac ctggcaactc aacaacgcag ttctccacct tcctggatgc
      181 gtcccatgtg agaatgataa cggcaccttg cactgctgga tacaagtaac accaaatgtg
      241 gctgtgaaac accgcggcgc gctcactcat aacctgcgga cgcacgtcga tatgatcgta
      301 atggcagcta cggtctgttc ggccctgtat gtgggagaca tgtgcggggc cgtgatgatt
      361 gtgtcgcagg ctctcataat atcgccagaa cgccacaact tcacccaaga gtgcaactgt
      421 tccatctacc aaggtcatat cactggccat cgcatggcat gggacatgat gctaaactgg
      481 tcaccaactc ttaccatgat ccttgcttat gccgctcgtg ttcccgagct agtccttgaa
      541 gttgtctttg gtggtcattg gggtgtggta tttggcttgg cctatttctc catgcaggga
      601 gcgtgggcca aggtcattgc catcctcctt cttgttgcgg gagtggacgc agagactcat
      661 accaccggct cagctgcggg tcatgccgca agggggtttg ccaacctcct tagtcctggt
      721 tccaagcaaa aaataagttt gatcaatacc aatggcagct ggcacataaa ccggaccgcc
      781 cttaattgta atgacagctt gcagacgggt ttcctcgctt ctttgtttta cgtcaacaac
      841 ttcaacagct ctggctgccc cgagcgcatg tcttcctgcc gtgggctgga cgatttccgc
      901 atcgggtggg gaaccctgga atacgagacc aacgtcacca acgaggagga catgaggccg
      961 tactgctggc attaccctcc gaagccttgc ggcattatct cggctaggac agtttgcggg
     1021 ccggtctact gtttcactcc tagccctgtt gttgtgggta ctactgacga gcagggcttg
     1081 cccacctata gctgggggga aaatgagacc gatgtcttcc tgttaaatag cacaagaccc
     1141 ccgcaaggag cttggttcgg ctgcacttgg atgaatggga ctgggtttac taagacatgc
     1201 ggtgcaccac cttgccgcat taggagggat ttcaacagca ccctcgatct actgtgcccc
     1261 acagactgct ttagaaagca cccggacacc acctatctca agtgtggagc agggccttgg
     1321 ttaaccccca gatgcctggt agactaccct tatagattgt ggcattatcc gtgcaccgta
     1381 aattttacca tctttaaggt gcggatgtat gtgggggggg tggagcatcg attgtcagca
     1441 gcatgcaact tcacgcgcgg ggaccgctgc agattggagg acagagatag gggtcagcag
     1501 agcccactgc tgcattccac cactgagtgg gcggtattac catgctcttt ctccgactta
     1561 ccggcactgt ccactggtct attgcacctc catcaaaata tcgtggacgt gcagtatctc
     1621 tatggactta ctccggctat cacaaaatac atcgtgaagt gggagtgggt ggttctcctc
     1681 tttttgctgt tggcagacgc cagggtctgt gcgtgccttt ggatgctcat catattgggc
     1741 caagccgaag cgtaatctag aaagtttaag ccgaattctg cagatatcca gcacagtggc
     1801 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     1861 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     1921 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     1981 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     2041 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagtggcg
     2101 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2161 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2221 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2281 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2341 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2401 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2461 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2521 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2581 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2641 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2701 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2761 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2821 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2881 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2941 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg
     3001 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc
     3061 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
     3121 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     3181 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag
     3241 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     3301 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     3361 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     3421 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     3481 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     3541 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     3601 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     3661 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttgat
     3721 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     3781 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     3841 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     3901 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     3961 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca
     4021 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     4081 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     4141 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     4201 ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     4261 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     4321 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     4381 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     4441 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     4501 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     4561 agctggttct ttccgcctca ggactcttcc tttttcaata aatcaatcta aagtatatat
     4621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     5641 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5701 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5761 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5821 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5881 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5941 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6001 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6061 tttaacaaaa tattaacgct tacaatttac gcgcgcgttg acattgatta ttgactagtt
     6121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     6181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     6241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     6301 tggactattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     6361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     6421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     6481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     6541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     6601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     6661 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     6721 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct
     6781 tggtaccgag ctcggatcca ctagtccagt gtggtggaat tcggctt
//
