LOCUS       sequence-180029-        7140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses syntaxin17 tagged with Myc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Uematsu M, Nishimura T, Sakamaki Y, Yamamoto H, Mizushima N
  TITLE     Accumulation of undegraded autophagosomes by expression of 
            dominant-negative STX17 (syntaxin 17) mutants.
  JOURNAL   Autophagy. 2017 Aug 3;13(8):1452-1464. doi: 
            10.1080/15548627.2017.1327940. Epub 2017 Jun 9.
  PUBMED    28598244
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2017.1327940"; journalName: "Autophagy"; date: 
            "2017-08-3- 3"; volume: "13"; issue: "8"; pages: "1452-1464"
COMMENT     Sequence Label: pMRXIP-3xMyc-STX17FL
FEATURES             Location/Qualifiers
     source          1..7140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..368
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             433..849
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     779..801
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     817..833
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    859..1233
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1171..1187
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1253..1282
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1289..1318
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1325..1354
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    2351..2903
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2495..2512)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2722..2741
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2915..3514
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2915..2934)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3411..3431
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3738..4207
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4337..4354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4508..5096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4588..4607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5260..6051,6052..6120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5883..5902
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6121..6225)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6293..6311
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        6413..6792
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 accgggaggt aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga
       61 ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc
      121 gtggtggaac tgacgagttc ggaacacccg gccgcaaccc tgggagacgt cccagggact
      181 tcgggggccg tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt tggactcttt
      241 ggtgcacccc ccttagagga gggatatgtg gttctggtag gagacgagaa cctaaaacag
      301 ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt
      361 gtctgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa
      421 aatatgggcc cgggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa
      481 gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc
      541 ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac
      601 cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca
      661 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
      721 aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc
      781 cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct
      841 ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc ccttgtaaac
      901 ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc tcacttacag
      961 gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta ccaagaacaa
     1021 ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg ggtccgccga
     1081 caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct gctgaccacc
     1141 cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca cgtgaaggct
     1201 gccgaccccg ggggtggacc atcctctaga ctgccggatc atcgccatgg gtgaacaaaa
     1261 gttgatttct gaagaagatt tgaacggtga acaaaagcta atctccgagg aagacttgaa
     1321 cggtgaacaa aaattaatct cagaagaaga cttgaacgct cgagaattgg ggaattcacg
     1381 cgttcgcgag ttaacggatc cgatgtctga agatgaagaa aaagtgaaat tacgccgtct
     1441 tgaaccagct atccagaaat tcattaagat agtaatccca acagacctgg aaaggttaag
     1501 aaagcaccag ataaatattg agaagtatca aaggtgcaga atctgggaca agttgcatga
     1561 agagcatatc aatgcaggac gtacagttca gcaactccga tccaatatcc gagaaattga
     1621 gaaactttgt ttgaaagtcc gaaaggatga cctagtactt ctgaagagaa tgatagatcc
     1681 tgttaaagaa gaagcatcag cagcaacagc agaatttctc caactccatt tggaatctgt
     1741 agaagaactt aagaagcaat ttaatgatga agaaactttg ctacagcctc ctttgaccag
     1801 atccatgact gttggtggag catttcatac tactgaagct gaagctagtt ctcagagttt
     1861 gactcagata tatgccttac ctgaaattcc tcaagatcaa aatgctgcag aatcgtggga
     1921 gacgttggag gcggacttga ttgaacttag ccaactggtc actgacttct ctctcctagt
     1981 gaattctcag caggagaaga ttgacagcat tgcagaccat gtcaacagtg ctgctgtgaa
     2041 tgttgaagag ggaaccaaaa acttagggaa ggctgcaaaa tacaagctgg cagctctgcc
     2101 tgtggcaggt gcactcatcg ggggaatggt agggggtcct attggcctcc ttgcaggctt
     2161 caaagtggca ggaattgcag ctgcacttgg tggtggggtg ttgggcttca caggtggaaa
     2221 attgatacaa agaaagaaac agaaaatgat ggagaagctc acttccagct gtccagatct
     2281 tcccagccaa actgacaaga aatgcagtta aggatccctc gagcggccgc tacgtaaatt
     2341 ccgcccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     2401 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     2461 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     2521 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     2581 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     2641 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     2701 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     2761 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     2821 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     2881 ctttgaaaaa cacgatgata atatggccac aaccatgacc gagtacaagc ccacggtgcg
     2941 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     3001 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     3061 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     3121 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     3181 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     3241 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     3301 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     3361 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     3421 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     3481 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     3541 gaccgaaagg agcgcacgac cccatggctc gacgataaaa taaaagattt tatttagtct
     3601 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     3661 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
     3721 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     3781 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     3841 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
     3901 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     3961 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     4021 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
     4081 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     4141 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     4201 tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta
     4261 aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4321 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4381 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4441 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4501 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4561 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4621 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4681 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4741 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4801 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4861 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4921 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4981 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5041 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5101 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5161 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgcgg ccggccgcaa
     5221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     5881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6241 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6301 tatcacgagg ccctttcgtc ttcactcgag gagcttggcc cattgcatac gttgtatcca
     6361 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
     6421 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     6481 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     6541 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     6601 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6661 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6721 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     6781 gctattacca tgctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     6841 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     6901 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgatt
     6961 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     7021 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     7081 ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc
//
