LOCUS       sequence-180032-        7303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide vector for integration of a constitutively expressed TetR by
            promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and
            2660694 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Lim B, Zimmermann M, Barry NA, Goodman AL
  TITLE     Engineered Regulatory Systems Modulate Gene Expression of Human 
            Commensals in the Gut.
  JOURNAL   Cell. 2017 Apr 20;169(3):547-558.e15. doi: 
            10.1016/j.cell.2017.03.045.
  PUBMED    28431252
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-20- 
            20"; volume: "169"; issue: "3"; pages: "547-558.e15"
COMMENT     Sequence Label: pExchange_tdk_BT1311p-tetR-2
FEATURES             Location/Qualifiers
     source          1..7303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(30..821,822..890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     653..672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             4759..5382
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4832..4851)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     rep_origin      6534..6922
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7093..7202
                     /note="incP origin of transfer"
ORIGIN
        1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
       61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc
      961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag
     1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag
     1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct
     1141 cttaaagaga gaaacatctt ttgcatacct tttccgaccg aatttttatg tcgtaaagag
     1201 gggctttgca gggggtggac tcagaaagat gagaatagat gactattgta gttgaaacac
     1261 atagaaagtt gctgatatac agaccgatac gcatatcggg atgaaccatg agtacgttct
     1321 tttctcaaaa aacataaata ttcgaaaaga gatgcaataa attaaggaga ggttataatg
     1381 aacaaagtaa atataaaaga tagtcaaaat tttattactt caaaatatca catagaaaaa
     1441 ataatgaatt gcataagttt agatgaaaaa gataacatct ttgaaatagg tgcagggaaa
     1501 ggtcatttta ctgctggatt ggtaaagaga tgtaattttg taacggcgat agaaattgat
     1561 tctaaattat gtgaggtaac tcgtaataag ctcttaaatt atcctaacta tcaaatagta
     1621 aatgatgata tactgaaatt tacatttcct agccacaatc catataaaat atttggcagc
     1681 ataccttaca acataagcac aaatataatt cgaaaaattg tttttgaaag ttcagccaca
     1741 ataagttatt taatagtgga atatggtttt gctaaaatgt tattagatac aaacagatca
     1801 ctagcattgc tgttaatggc agaggtagat atttctatat tagcaaaaat tcctaggtat
     1861 tatttccatc caaaacctaa agtggatagc acattaattg tattaaaaag aaagccagca
     1921 aaaatggcat ttaaagagag aaaaaaatat gaaacttttg taatgaaatg ggttaacaaa
     1981 gagtacgaaa aactgtttac aaaaaatcaa tttaataaag ctttaaaaca tgcgagaata
     2041 tatgatataa acaatattag tttcgaacaa tttgtatcgc tatttaatag ttataaaata
     2101 tttaacggct aaaaacaata ggccacatgc aactgtaaat gtttacgcgt cctcggtacc
     2161 gcttcttcca caacagtctg cggttcctgt actatcacag gttcatcttc tcccggtgct
     2221 gtcgcttcat tctcttccac gaccttctct tctatcaccg gacattcaac ttcctctatt
     2281 acaggggctt gttcttcaac aatgggggct tcactttccg cttccgctac aggagaaggc
     2341 gaggcttcca ccggcacagc acttatctct tccgacaact gttccaaacg ctcctgcata
     2401 cgtaggatgc tccgcttcaa cagttcagac aaagtctgag tcggctcttt agaaaacgta
     2461 ttcatgagta gcttcagctc atgaacatcc agctcaatat ctgttaatag cttctgtttc
     2521 atttccatcc attgttatta tggtgcgaat gtaggaataa tttatgcaat aacttaaaat
     2581 aatggataat ttaacagttc ttgtacatcg aattgtttac ctttgtccac tcatttattt
     2641 aatcaagaaa gcacaaaaat cacatggtat tattttcaga agaccacata caagagacca
     2701 gaagaagagg tagaatagag gtaatctgcg gttcaatgtt ctccggaaag acggaggaat
     2761 tgattcgtag gatgaagaga gccaagttcg cacgccagcg tgtagagata ttcaaaccgg
     2821 ccatcgacac ccgttattcg gaagaagacg tggtatcaca cgacagtcat tccatcgcgt
     2881 ccacccctat cgattcatcg gctagtatcc tgttgttcac ttctgaaata gatgtagtcg
     2941 gcatcgacga agcacagttc tttgatgacg gactgattga cgtatgcagg cagcttgcca
     3001 ataacggcat ccgtgtcatc atcgccggac tggatatgga ctttaaaggg aatcccttcg
     3061 gaccgatgcc tcaattatgt gccattgccg atgaagtttc caaagttcat gcgatctgcg
     3121 tgaagtgcgg tgatctggca tctttctctc atcgtaccgt caagaatgac aagcaggtac
     3181 ttctaggaga gacagccgaa tacgagccgc tttgcaggga gtgctacctc cgtgcacggg
     3241 gagaggacgg acagaagata taaactctat ttaaaacata tatggaaaga aagaagataa
     3301 cattcgagtc gacggtatcg ataagcttga tatcgaattc ctgcaacaac tcaacccgga
     3361 caaaggaacg atccaaagtt tgtatgataa agaattgaat tgtgaactgg tcgatagttc
     3421 gtctccatgg gaactgggtg cttttatcta tgagaaatta ggtaatcgtg accaattggc
     3481 ccaatatcgc ctggatgatt acaacagaac aggattgagt gagtgccgga tcatcgacag
     3541 ttcgaatgga cctatttatc aacgtatcct gttgcaggga aaatctccgg gaacagacga
     3601 agcattcggg gtcaatctgg aaattaaatt atatcatgat accaaacgaa tagaactgga
     3661 gtacgcaatc aagcgtctgc cggaaacgga tccctcaggg atatacgttg cttttccctt
     3721 ccaactgccc gaaggaaaac tggcattcga cgttcagggc ggaacagtta tatctggcaa
     3781 gaatcaactg gaagggacat ctacagactg gaatactgtt caaaactttg tatcggtaca
     3841 aaataatcag gcgcaaatta ttataggaag cagcctcatc cccctctatc aactaggaga
     3901 cataaatctc ggtaaatttc aatatcagaa acaatacgaa aggcctcatg tctactcgtg
     3961 ggtgatgaat aattactgga ataccaactt caaagcttcc caagaaggag agttccgttg
     4021 gagttaccat ctgacctctt ccgttgaccc atctaacaac cttgctacca agtttggctg
     4081 gagctcacgt attcctctat atgcccgggt aatgccgaca ggaacagaga atcataaacc
     4141 cacagagtac tcagccttcc attttgagaa agacaatttc ctgatgactt catgtacccc
     4201 ctctaaagaa gaaggttata tactactgaa cattagagag atagatggaa agagaaccaa
     4261 cctcaggatt ttaaatgaag acgggcaaac catccccttc acaatagtaa acgctattga
     4321 agagaaattg gaaaatgaaa cgtcagagca tatatttgag gggtaccaga ataaatttat
     4381 aaagataaaa cgtttttaat caaaagaatc aaatgcgtag tctaccctaa aaaacggaag
     4441 aatcatcacc acaaagagtg atctggaaga agcaatgaaa gctgctgtta agtctccgaa
     4501 tcaggtattg ttcctgacag gtgtattccc atccggtaaa cgcggatact ttgcagttga
     4561 tctgactcag gaataaatta taaattaagg taagaagatt gtaggataag ctaatgaaat
     4621 agaaaaagga tgccgtcaca caacttgtcg gcattctttt ttgttttatt agttgaaaat
     4681 atagtgaaaa agttgcctaa atatgtatgt taacaaatta tttgtcgtaa ctttgcactc
     4741 caaatctgtt tttaaagaat gtctagatta gataaaagta aagtgattaa cagcgcatta
     4801 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
     4861 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
     4921 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
     4981 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
     5041 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
     5101 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
     5161 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
     5221 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
     5281 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
     5341 tgcggattag aaaaacaact taaatgtgaa agtgggtctt aaacgaggag atgtcaaaag
     5401 tcagtagcaa ctctcctaat actattgact tttgcccctc atcatctgtc agaacgaccg
     5461 gaaagtatct gctccaaact ttcatcgacc actcccgcca actgaagatt ggacagcaag
     5521 cggtcataag aaatcctccc tactccttcc attttcagca gccatctcca tccctttccc
     5581 gatactaatt ccagcagttg ccctaccgtc cggatttctt ctttatacaa gacattagtc
     5641 agcctcgtat cggccacttt gccgacagac aattggagta ccctttcttc ctgattcaaa
     5701 tttatattct tgcttttatc cgccaaagac tgaaaacggg catctatgat atgtttccga
     5761 taagtagcca acacattctt tctcactttc agtcctctga gatgagaatt ataaatctgt
     5821 aacgccctgt cataagtaat tcgatgccga ccggcaactt tctctattga ctcccccata
     5881 gagatggagt agaaaatatc acgggctccg ggatgaacaa tcaattggga aagttcacga
     5941 ataataattt catatagagg tttacacgtt ttctgtgttc gcaatacata tagcagatca
     6001 tcatatctgg aaataataaa gtcgcgttcc agctttttct cttccacaat ctttgccaga
     6061 taatccgctt gcaatccaag ccttttgtga gcttcgagat aggcagtcag actttcgtca
     6121 tccaagaaca gttggttgcc catccggcaa ctggtaatgt aaccgagatt cgcccagttt
     6181 cgcagagttg cttcctgaac gccatgtctt tcagacattt gttttgttgt gagccaatag
     6241 gacataaaaa aacggaacta agttaatact cttacctagt tccgtacctt ttatattata
     6301 aaagagtacg gagctgtcac atcccgctaa taggaggtag taacggctac ctgatgtata
     6361 cgggcagaca gcccgtaccc tatgagaagt catagaagta caggcgtctt cccggcatac
     6421 atcaaaaaaa tccgttacaa tttcctatta gccgaggaaa cgcgcaatcg cacaccttta
     6481 aacggtatgc tacgttccga tgacaaagcc accgcggtgg aggggaattc ccatgtcagc
     6541 cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc ttctcagtgc
     6601 gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa aagccttata
     6661 tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata
     6721 ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta
     6781 gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt
     6841 acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg
     6901 ttgaactgct gatcttcaga tcctctacgc cggacgcatc gtggccggat caattccgtt
     6961 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
     7021 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
     7081 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
     7141 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
     7201 ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag
     7261 gaagggcagc ccacctatca cggaattgat ccccctcgaa ttg
//
