LOCUS       sequence-180033-        7290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide vector for integration of a constitutively expressed TetR by
            promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and
            6193957 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Lim B, Zimmermann M, Barry NA, Goodman AL
  TITLE     Engineered Regulatory Systems Modulate Gene Expression of Human 
            Commensals in the Gut.
  JOURNAL   Cell. 2017 Apr 20;169(3):547-558.e15. doi: 
            10.1016/j.cell.2017.03.045.
  PUBMED    28431252
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-20- 
            20"; volume: "169"; issue: "3"; pages: "547-558.e15"
COMMENT     Sequence Label: pExchange_tdk_BT1311p-tetR-3
FEATURES             Location/Qualifiers
     source          1..7290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(30..821,822..890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     653..672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             4755..5378
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4828..4847)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     rep_origin      6521..6909
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7080..7189
                     /note="incP origin of transfer"
ORIGIN
        1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
       61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc
      961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag
     1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag
     1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct
     1141 cttaaagaga gaaacatctt ttgcatacct tttccgaccg aatttttatg tcgtaaagag
     1201 gggctttgca gggggtggac tcagaaagat gagaatagat gactattgta gttgaaacac
     1261 atagaaagtt gctgatatac agaccgatac gcatatcggg atgaaccatg agtacgttct
     1321 tttctcaaaa aacataaata ttcgaaaaga gatgcaataa attaaggaga ggttataatg
     1381 aacaaagtaa atataaaaga tagtcaaaat tttattactt caaaatatca catagaaaaa
     1441 ataatgaatt gcataagttt agatgaaaaa gataacatct ttgaaatagg tgcagggaaa
     1501 ggtcatttta ctgctggatt ggtaaagaga tgtaattttg taacggcgat agaaattgat
     1561 tctaaattat gtgaggtaac tcgtaataag ctcttaaatt atcctaacta tcaaatagta
     1621 aatgatgata tactgaaatt tacatttcct agccacaatc catataaaat atttggcagc
     1681 ataccttaca acataagcac aaatataatt cgaaaaattg tttttgaaag ttcagccaca
     1741 ataagttatt taatagtgga atatggtttt gctaaaatgt tattagatac aaacagatca
     1801 ctagcattgc tgttaatggc agaggtagat atttctatat tagcaaaaat tcctaggtat
     1861 tatttccatc caaaacctaa agtggatagc acattaattg tattaaaaag aaagccagca
     1921 aaaatggcat ttaaagagag aaaaaaatat gaaacttttg taatgaaatg ggttaacaaa
     1981 gagtacgaaa aactgtttac aaaaaatcaa tttaataaag ctttaaaaca tgcgagaata
     2041 tatgatataa acaatattag tttcgaacaa tttgtatcgc tatttaatag ttataaaata
     2101 tttaacggct aaaaacaata ggccacatgc aactgtaaat gtttacgcgt cctcggtacc
     2161 gcttcttcca caacagtctg cggttcctgt actatcacag gttcatcttc tcccggtgct
     2221 gtcgcttcat tctcttccac gaccttctct tctatcaccg gacattcaac ttcctctatt
     2281 acaggggctt gttcttcaac aatgggggct tcactttccg cttccgctac aggagaaggc
     2341 gaggcttcca ccggcacagc acttatctct tccgacaact gttccaaacg ctcctgcata
     2401 cgtaggatgc tccgcttcaa cagttcagac aaagtctgag tcggctcttt agaaaacgta
     2461 ttcatgagta gcttcagctc atgaacatcc agctcaatat ctgttaatag cttctgtttc
     2521 atttccatcc attgttatta tggtgcgaat gtaggaataa tttatgcaat aacttaaaat
     2581 aatggataat ttaacagttc ttgtacatcg aattgtttac ctttgtccac tcatttattt
     2641 aatcaagaaa gcacaaaaat cacatggtat tattttcaga agaccacata caagagacca
     2701 gaagaagagg tagaatagag gtaatctgcg gttcaatgtt ctccggaaag acggaggaat
     2761 tgattcgtag gatgaagaga gccaagttcg cacgccagcg tgtagagata ttcaaaccgg
     2821 ccatcgacac ccgttattcg gaagaagacg tggtatcaca cgacagtcat tccatcgcgt
     2881 ccacccctat cgattcatcg gctagtatcc tgttgttcac ttctgaaata gatgtagtcg
     2941 gcatcgacga agcacagttc tttgatgacg gactgattga cgtatgcagg cagcttgcca
     3001 ataacggcat ccgtgtcatc atcgccggac tggatatgga ctttaaaggg aatcccttcg
     3061 gaccgatgcc tcaattatgt gccattgccg atgaagtttc caaagttcat gcgatctgcg
     3121 tgaagtgcgg tgatctggca tctttctctc atcgtaccgt caagaatgac aagcaggtac
     3181 ttctaggaga gacagccgaa tacgagccgc tttgcaggga gtgctacctc cgtgcacggg
     3241 gagaggacgg acagaagata taaactctat ttaaaacata tatggaaaga aagaagataa
     3301 cattcgagtc gacggtatcg ataagcttga tatcgaattc ctgcacatcg tgaagcagat
     3361 cctgagctgt atcacggtcg ccggtatagc gcagacagac gcctagcaaa cgccctccat
     3421 aatgctcata caattctttg cgggctcgat tatttcccag cctgcattgt tccgacaact
     3481 caaattcttc catttgctta tacacgtgtt taccttataa atgcagcaaa gatagaaata
     3541 ctgcacagag agaacaactc ttttgcaact tttgtttctt taacagatcc aaatgcaata
     3601 ttttctcccc ccttgcattt tcagagtaca aagcaagtaa atagtactca ttaaaaaaca
     3661 tttttatagt atatgaagaa atttaaattc attgcattta ttattgctgt tatgaccaca
     3721 atgccgatac ttcagtcgtg tctggatgat gatgactcct catccgattc actggtcatc
     3781 agcaccatca atctgatcag cccggatagc aaggatttct atttcactct ggatgatgga
     3841 aaaacaatgt tcccgagcaa tggaaacgga tggatcagtg acaagaataa agacgggcaa
     3901 cgcgcctttg tgatattcaa agagctggaa gaaccggtga agggatatga ctataatatt
     3961 caggtaagag aaataaaaga gatcctgacc aaagaaatcg tgactatggg ggaaggagaa
     4021 aatacggaag agaagatcgg agacgataaa atcaattcta cttatatgtg gattacccaa
     4081 gacaagaaat atctgacaat cgaattccaa tattacggca cgcatagcga ggacaagaaa
     4141 catttcctga atctggtcat caacaacaag gaagctgaac ccgctgccga tgatgccaac
     4201 gatgagtata tcgatcttga attccggcat aacgacgaag gcgacaccgc cgaccgtctg
     4261 ggcgaagggt atatatcttt caaactggat aagattcaag atcagatgaa aggcaagaaa
     4321 ggactcagaa tacgtgtcaa gaccttatat aacggggaaa aattctatga agtgaaattc
     4381 ccctcatcta attaaatcta gtaattatag aaacactctt tttattataa tatataatta
     4441 cgcaaaagag ggcgtgatct ggaagaagca atgaaagctg ctgttaagtc tccgaatcag
     4501 gtattgttcc tgacaggtgt attcccatcc ggtaaacgcg gatactttgc agttgatctg
     4561 actcaggaat aaattataaa ttaaggtaag aagattgtag gataagctaa tgaaatagaa
     4621 aaaggatgcc gtcacacaac ttgtcggcat tcttttttgt tttattagtt gaaaatatag
     4681 tgaaaaagtt gcctaaatat gtatgttaac aaattatttg tcgtaacttt gcactccaaa
     4741 tctgttttta aagaatgtct agattagata aaagtaaagt gattaacagc gcattagagc
     4801 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
     4861 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
     4921 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
     4981 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
     5041 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
     5101 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
     5161 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
     5221 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
     5281 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
     5341 gattagaaaa acagctgaaa tgtgaatctg gatcttaaac agttgtgaaa ctctcgccct
     5401 taattttatc ataaagtgat ccgtttattt cttcttatta tttcttttca ttcttgtcac
     5461 cgaaataatt ccagacgagc gcactgacag ccgctcctac aaacggcgct acaataaaca
     5521 gccacaactg cgacaacgct tctccaccct ggaacaatgc cggaccgatg ctacgtgccg
     5581 gattgacgga agtacctgta atagggatac atacgatatg caccaatacc agagaaagac
     5641 cgatggcaag gcctgcgaaa gctcccgccc ctttcttcgg atcggtagac cccagcacca
     5701 ccaatacaaa aatgaaagta aagaccactt ctgcgatgaa tgcctgtagc atctcgccgt
     5761 caccaaagcc gttggaaccg gtagccgtcg gtccgtcatg cccgccggtg gtcaccaggg
     5821 cataaagaat agccgaaccg ataatcgctc ctataacctg gaaaagcata tacatacccg
     5881 catcttttcc attcatacga cccgagagga atactcctaa cgtaatagca ggattgatgt
     5941 gacatcccga aatacctccg atagcatacg ccatcgccac tactgaaagt ccaaatgcca
     6001 aagcaacgcc gaccgttccc acgccggctc ctacagtacc agccatactg ccggcaaaaa
     6061 cagcacttcc acatcccatc aggacgagca ccatcgtccc aatcatttct gcaatgtatt
     6121 ttttcatatt actaaataaa aaatggttga ttctccaaat ataaggaatt aatacctaga
     6181 ttcaaaatgt atgtgcctaa ttattactat ctttgcagca tttttgaata aaaccaccaa
     6241 accataaaat gaaaaaagga cttttttatg ctatactggc ctctgtatta tgggctatcg
     6301 tcaatccgtt tatcaagcag gggctgagtt acgacttttc gccgatgaac tttgcaggaa
     6361 tccgctttac cactgtaggt atcattcttt ttgcttatac ctggcataag ggaatgtgga
     6421 aagaaatacg ccaacacagc aaactgtttc tgaatctgat cctgatcaat atgtttatgg
     6481 ggtacacggc tttcgccacc gcggtggagg ggaattccca tgtcagccgt taagtgttcc
     6541 tgtgtcactc aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg
     6601 cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat tctttttttt
     6661 cttataaaac ttaaaacctt agaggctatt taagttgctg atttatatta attttattgt
     6721 tcaaacatga gagcttagta cgtgaaacat gagagcttag tacgttagcc atgagagctt
     6781 agtacgttag ccatgagggt ttagttcgtt aaacatgaga gcttagtacg ttaaacatga
     6841 gagcttagta cgtgaaacat gagagcttag tacgtactat caacaggttg aactgctgat
     6901 cttcagatcc tctacgccgg acgcatcgtg gccggatcaa ttccgttttc cgctgcataa
     6961 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     7021 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     7081 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     7141 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc
     7201 gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca
     7261 cctatcacgg aattgatccc cctcgaattg
//
