LOCUS       sequence-180043-        6829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP3.1.2
FEATURES             Location/Qualifiers
     source          1..6829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1845..1862)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1851..2075
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2207..2795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2287..2306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3124..3171
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3196..3215
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3403..4191)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3514..3533)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4118..4137
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4226..4583)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4240..4375
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4392..4583
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4540..4560
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4643..5434,5435..5503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5266..5285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5504..5608)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5635..6090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5722..5741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5932..5953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6102..6481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6482..6685
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6636..6656
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6730..6749
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6730..6748
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcataggatc catgggttgc tctttttcta tcttccttct tgctctgttc tcttgcctaa
       61 tccatccagc agctagttta gagtggcgga acacgtctgg cctctacatc cttaccaacg
      121 actgccccaa tagcagtatt gtgtatgagg ccgatgacgt tattctgcac acacctggct
      181 gtataccttg tgttcaggac ggcaatacat ccacgtgctg gacctcagtg acacctacag
      241 tggcagtcag atacgtcgga gctaccaccg cttcgatacg cagtcatgta gacctgctgg
      301 taggcgcggc cacgatgtgc tctgcgctct acgtgggtga cgcgtgtggg gccgtcttcc
      361 tcgtgggaca ggccttcacg ttcagacccc gtcgccatca aacggtccag acctgcaact
      421 gctcgctgta cccaggccat ctcacgggac atcgaatggc ttgggatatg atgatgaatt
      481 ggtcccccgc tgtgggtatg gtagtggcgc acgtcctgcg tctgccccag accttgttcg
      541 acataatagc cggggcccat tggggcatct tggcgggcct agcctattac tccatgcagg
      601 gcaactgggc caaggtcgtt atcatcatgg ttatgttttc aggggttgac gcttctacat
      661 acaccaccgg tggccaagca gctcgtggtg cccgtgggat agctggtctt tttgatctgg
      721 gcgcccgaca gaacctgcag ctggttaaca ccaatggctc gtggcacatc aacaggacag
      781 ccctgaactg caatgagtcc ataaacacgg ggttcatagc agggttgttt tactaccata
      841 agttcaactc tactgggtgt cctcaaaggc tcagcagctg caagcccatc acttccttca
      901 ggcaggggtg gggccctttg acagatgcta acatcaccgg ttcctctgat gacaaaccgt
      961 actgctggca ctacgcacct agaccttgtg agtctgtccc agcatcaaaa gtctgcggcc
     1021 ctgtgtactg cttcacacca tcgccagtgg ttgtaggcac tactgatgct aagggcgtcc
     1081 caacctacac ctggggtgcg aatgagacag acgtgttcct gctgaattcc ctgcggcctc
     1141 ccaaaggtcg gtggttcggg tgcacctgga tgaactctac ggggtttacc aagacgtgcg
     1201 gagccccccc ttgtaacatc tatgggggtg gggggaattc caaaaatgag tcagacctct
     1261 tctgccccac cgactgcttc aggaaacatc ctgaggccac atacagccgg tgtggtgcgg
     1321 ggccctggtt gacacctcgg tgcatggtcg actatccata ccggctttgg cactacccat
     1381 gtacagtcaa tttcacattg ttccaggtga ggatgtttgt gggcgggttt gaacaccggt
     1441 ttaccgccgc ttgcaactgg accagggggg agcgctgcga tatcgaggat cgcgaccgca
     1501 gcgagcaaca tccgctgcta cattcaacaa ctgagctcgc tatactgcct tgctccttca
     1561 cgcccatgcc cgcattgtcg acaggtctaa tacacctcca ccaaaacatc gtggatgtcc
     1621 aatacctcta tggcgttgga tctggcatgg tgggatgggc gttgaaatgg gagttcgttg
     1681 tcctcgtctt cctcctccta gcagatgcac gcgtgtgcgt tgccctttgg ctgatgctga
     1741 tgatttcaca ggcggaagca taagcttata agccgaattc tgcagatatc cagcacagtg
     1801 gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     1861 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     1921 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     1981 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     2041 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagtgg
     2101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2641 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2941 tatatgagta acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg
     3001 ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc
     3061 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
     3121 tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc
     3181 gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga
     3241 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga
     3301 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt
     3361 tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag
     3421 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     3481 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     3541 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     3601 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     3661 gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttg
     3721 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     3781 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3841 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     3901 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3961 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt
     4021 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     4081 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     4141 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     4201 tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc
     4261 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     4321 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     4381 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4441 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4501 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4561 acagctggtt ctttccgcct caggactctt cctttttcaa taaatcaatc taaagtatat
     4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
     5641 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5701 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5761 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5821 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5881 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5941 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6001 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6061 attttaacaa aatattaacg cttacaattt acgcgcgcgt tgacattgat tattgactag
     6121 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     6181 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     6241 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     6301 ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     6361 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     6421 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     6481 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     6541 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     6601 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     6661 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     6721 tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgtt taaacttaag
     6781 cttggtaccg agctcggatc cactagtcca gtgtggtgga attcggctt
//
