LOCUS       sequence-180059-       17028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) 
            under CaMV 35S promoter and empty gRNA scaffold, Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L
  TITLE     CRISPR/Cas9-mediated base-editing system efficiently generates 
            gain-of-function mutations in Arabidopsis.
  JOURNAL   Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.
  PUBMED    28303459
REFERENCE   2  (bases 1 to 17028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci China 
            Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5."
COMMENT     Sequence Label: pXSE901B
FEATURES             Location/Qualifiers
     source          1..17028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             957..1748
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1900..1975
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2589..2933
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3083..3769
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(3818..3841,3842..3844,3845..7918)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5601..5609
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7931..8179
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8192..8212
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        8969..9645
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    10749..10773
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11198..11992
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12079..12667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12568..12587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12821..12838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12853..12993
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12919..12938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13079..13101
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13337..13531
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13597..14670)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15099..15728)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttcgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct
      301 tcttcttcgt tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct
      361 ttcgttttct tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc
      421 ccaggattag aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca
      481 ttcttaagat atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag
      541 gcccatttat atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac
      601 aatcttcaaa agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta
      661 gagtcgaagt agtgattggg agaccaaccc agtggacata agcctgttcg gttcgtaagc
      721 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      781 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
      841 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      901 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
      961 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     1021 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     1081 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     1141 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     1201 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1261 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     1321 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1381 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     1441 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     1501 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     1561 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     1621 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     1681 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     1741 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     1801 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     1861 cttttattat ttttaagcgt gcataataag ccggtctcgg ttttagagct agaaatagca
     1921 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     1981 tttgcaaaat tttccagatc gatttcttct tcctctgttc ttcggcgttc aatttctggg
     2041 gttttctctt cgttttctgt aactgaaacc taaaatttga cctaaaaaaa atctcaaata
     2101 atatgattca gtggttttgt acttttcagt tagttgagtt ttgcagttcc gatgagataa
     2161 accaataagc ttgcatgcct gcaggtcaac atggtggagc acgacacact tgtctactcc
     2221 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg
     2281 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
     2341 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
     2401 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     2461 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgataacatg
     2521 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     2581 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     2641 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     2701 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     2761 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     2821 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     2881 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacctcgacc
     2941 tcaacacaac atatacaaaa caaacgaatc tcaagcaatc aagcattcta cttctattgc
     3001 agcaatttaa atcatttctt ttaaagcaaa agcaattttc tgaaaatttt caccatttac
     3061 gaacgatact cgagtaatct agatgtcttc cgagacagga ccggttgccg tcgaccctac
     3121 tcttagaagg cgcattgagc cacacgagtt cgaggtgttc ttcgatccga gagagctgag
     3181 gaaggagact tgcctccttt acgagatcaa ttggggcgga aggcactcta tttggcgcca
     3241 tacctcccag aacacaaata agcatgtgga ggttaatttc atcgagaagt tcactaccga
     3301 gaggtacttc tgcccaaaca cacgctgcag catcacttgg ttccttagct ggtcaccgtg
     3361 cggagagtgc tctcgcgcca ttacagagtt cctgtccaga tacccgcacg ttactctttt
     3421 catctacatt gccagactgt accaccatgc ggatcctcgc aacagacagg gtcttaggga
     3481 cctgatcagc tcaggcgtca ccatccagat tatgacagag caggagtctg gatactgctg
     3541 gcgcaacttc gtgaattact ctccttccaa tgaggctcac tggccaagat acccgcatct
     3601 gtgggtcagg ctctacgtgc tcgagcttta ctgcatcatt cttggtctgc ctccatgcct
     3661 caacatcctt agaaggaagc agccacagct cacattcttc actattgccc ttcagtcttg
     3721 ccactaccag aggcttccgc ctcatattct gtgggcgact ggcctcaaga gcggctcaga
     3781 gactccggga acatctgagt ccgctactcc tgagtctgac aagaagtact ccatcggact
     3841 cgccattggt actaactccg ttggatgggc ggtcatcacc gatgagtaca aggtgcctag
     3901 caagaagttc aaggttcttg gtaacacaga cagacactca atcaagaaga atctgattgg
     3961 tgctctgctc ttcgattctg gagagactgc cgaggctacc aggctcaaga gaaccgcccg
     4021 cagaaggtac acacgcagaa agaataggat ctgctacctt caggagattt tctctaacga
     4081 gatggctaag gttgatgaca gcttcttcca tcgccttgag gagtcattcc tggtcgagga
     4141 ggacaagaag cacgagagac atcctatctt cggtaacatt gtcgatgagg tggcctacca
     4201 cgagaagtac ccaactatct accatcttag gaagaagctg gtggatagca ccgacaaggc
     4261 ggatctccgc cttatctacc tggctctcgc ccacatgatt aagttcagag gccatttcct
     4321 catcgagggc gatctcaacc cagataattc agacgtcgat aagctcttca tccagcttgt
     4381 gcagacatac aatcagcttt tcgaggagaa cccgattaat gcgagcggtg ttgatgcgaa
     4441 ggctatcctg tcagctagac tcagcaagtc aaggcgcctg gagaacctca tcgcccagct
     4501 gccaggcgag aagaagaacg gtcttttcgg caatctgatt gcgctttctc tgggactcac
     4561 cccgaacttc aagtccaatt tcgacctggc tgaggatgcc aagctccagc tgtctaagga
     4621 tacatacgat gacgatctcg acaaccttct ggctcagatc ggcgaccagt acgccgatct
     4681 cttccttgct gccaagaatc ttagcgatgc catcctcctt tcagacattc tgagagttaa
     4741 cactgagatt accaaggctc cgctgtctgc ctccatgatc aagagatacg atgagcacca
     4801 tcaggacctc actctgctca aggcgctggt ccgccagcag ctccctgaga agtacaagga
     4861 gatcttcttc gaccagtcta agaacggcta cgcgggttac attgatggtg gcgctagcca
     4921 ggaggagttc tacaagttca tcaagccaat tctggagaag atggatggca ctgaggagct
     4981 tctggtcaag ctcaataggg aggatctcct taggaagcag cgcaccttcg acaacggatc
     5041 tatccctcac cagattcatc ttggagagct gcacgccatc ctcagaaggc aggaggattt
     5101 ctacccattc cttaaggaca accgcgagaa gatcgagaag attctgactt tcagaatccc
     5161 ttactacgtt ggcccgctcg ctagaggcaa ctctaggttc gcgtggatga ccaggaagtc
     5221 agaggagact atcacccctt ggaacttcga ggaggtggtt gacaagggag ccagcgcgca
     5281 gtcattcatt gagcgcatga ctaatttcga taagaacctg cctaatgaga aggtcctccc
     5341 aaagcatagc ctgctctacg agtacttcac tgtgtacaac gagcttacca aggtgaagta
     5401 tgtgacagag ggcatgcgca agccggcttt cctttcagga gagcagaaga aggccatcgt
     5461 ggatcttctg ttcaagacta atagaaaggt caccgtgaag cagctgaagg aggattactt
     5521 caagaagatt gagtgcttcg actctgttga gatctccggt gtcgaggata ggttcaacgc
     5581 ttccctcggc acctaccacg acctccttaa gatcattaag gacaaggatt tcctggataa
     5641 cgaggagaat gaggacatcc tcgaggatat tgtgctgaca ctcactcttt tcgaggacag
     5701 ggagatgatc gaggagcgcc ttaagacata cgcgcatctg ttcgacgata aggttatgaa
     5761 gcagctcaag cgcagaaggt acactggatg gggtagactc tctaggaagc tcatcaacgg
     5821 catcagagat aagcagtctg gcaagactat tctcgatttc cttaagtccg acggcttcgc
     5881 taacaggaat ttcatgcagc tcattcacga cgattctctt actttcaagg aggacatcca
     5941 gaaggcgcag gttagcggcc agggagattc actgcacgag catatcgcga acctcgctgg
     6001 ctcccctgct atcaagaagg gcatcctcca gaccgttaag gtcgtggatg agctggttaa
     6061 ggtcatgggc agacataagc cagagaacat cgtcattgag atggccaggg agaatcagac
     6121 aactcagaag ggacagaaga actctaggga gcgcatgaag agaatcgagg agggtattaa
     6181 ggagcttggc tcccagatcc tgaaggagca cccggtggag aacacacagc tgcagaatga
     6241 gaagctgtac ctctactacc tccagaatgg ccgcgacatg tatgtggatc aggagcttga
     6301 cattaacaga ctttctgact acgatgtgga ccatatcgtt ccacagtctt tccttaagga
     6361 cgattccatt gataataagg tgctgactag atccgataag aacaggggaa agtctgacaa
     6421 tgttccgtcc gaggaggttg tcaagaagat gaagaactac tggaggcagc tgctcaatgc
     6481 taagctcatc acccagagga agttcgacaa ccttacaaag gccgagcgcg gaggtctgag
     6541 cgagcttgat aaggcgggtt tcattaagag acagctcgtt gagacaaggc agatcactaa
     6601 gcacgtcgcc cagattcttg actcaaggat gaacaccaag tacgacgaga atgataagct
     6661 gatccgcgag gtgaaggtta ttacactgaa gagcaagctc gtttcagatt tcagaaagga
     6721 cttccagttc tacaaggtca gggagatcaa caattaccac catgcccatg atgcgtacct
     6781 caacgcggtg gttggtactg ctcttattaa gaagtacccg aagctggagt ctgagttcgt
     6841 gtacggcgat tacaaggtgt acgacgttag aaagatgatc gctaagagcg agcaggagat
     6901 tggcaaggct accgccaagt acttcttcta ctcaaacatt atgaatttct tcaagacaga
     6961 gatcactctc gcgaacggcg agatcagaaa gaggccactt attgagacta acggcgagac
     7021 aggagagatc gtctgggata agggtcgcga cttcgctact gtcagaaagg tgctctctat
     7081 gccgcaggtt aatattgtca agaagactga ggtgcagacc ggcggattct ctaaggagtc
     7141 cattctccct aagaggaact ccgacaagct catcgcccgc aagaaggatt gggaccctaa
     7201 gaagtacggt ggcttcgata gcccaaccgt cgcttactca gtgcttgtcg tggccaaggt
     7261 cgagaaggga aagagcaaga agctgaagtc agtgaaggag cttctgggta tcacaattat
     7321 ggagaggtct tccttcgaga agaatcctat cgacttcctc gaggcgaagg gctacaagga
     7381 ggttaagaag gatcttatca ttaagctgcc aaagtactca cttttcgagc tggagaacgg
     7441 acgcaagaga atgctggcgt ctgctggaga gcttcagaag ggtaatgagc ttgctctgcc
     7501 gtctaagtat gtgaacttcc tctaccttgc ctctcattac gagaagctca agggctcccc
     7561 tgaggacaac gagcagaagc agctgttcgt cgagcagcac aagcattacc tcgatgagat
     7621 cattgagcag attagcgagt tctcaaagag agtgatcctc gccgatgcga atctcgacaa
     7681 ggttcttagc gcgtacaaca agcaccgcga taagccaatc agagagcagg ctgagaatat
     7741 cattcatctc ttcaccctta caaacctggg tgctccggcg gctttcaagt acttcgatac
     7801 cacaattgac aggaagcgct acacttcaac caaggaggtg ctggacgcca ccctcatcca
     7861 ccagtctatt actggcctct acgagactag gatcgatctc tcccagcttg gtggtgactc
     7921 tggcggatcc accaacctca gcgatatcat tgagaaggag acaggcaagc agcttgttat
     7981 ccaggagtca attctgatgc tcccggagga ggtggaggag gttattggca ataagcctga
     8041 gtctgatatc ctcgtgcata ctgcctacga tgagagcacc gacgagaacg ttatgctcct
     8101 tacatcagac gcgcctgagt acaagccttg ggctctcgtc attcaggatt ccaacggaga
     8161 gaataagatc aagatgctta gcggtggctc tcctaagaag aagagaaagg tgtgagctca
     8221 gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca
     8281 ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt
     8341 cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa
     8401 ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct
     8461 caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga
     8521 gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa
     8581 gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa
     8641 atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg
     8701 ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat
     8761 cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt
     8821 aatgcatttt atgacttgcc aattgattga caacgaattc gaggcggttt gcgtattggc
     8881 tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
     8941 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
     9001 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
     9061 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
     9121 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
     9181 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact
     9241 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
     9301 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
     9361 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
     9421 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     9481 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     9541 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     9601 tcctctatat aaggaagttc atttcatttg gagaggacct cgacaccatc ttccacacac
     9661 tcaagccaca ctattggaga acacacaggg acaacacacc ataagatcca agggaggcca
     9721 ccgccgccgc gccggtaacc accccgcccc tctcctcttt ctttctccgt ttgatctttc
     9781 tttcttcttt ttgtgggtag aatttgaatc cctcagcatt gttcatcggt agtttttctt
     9841 ttcatgattt gtgacaaatg cagcctcgtg cggagctttt ttgtaggtag aagctagatg
     9901 agccccgaga ggaggccagc ggacattagg agggcgactg aggcggatat gccagccgtc
     9961 tgcaccatcg tcaatcacta catcgaaacc tccacggtga acttcaggac agagccgcag
    10021 gagccccagg agtggactga cgatctcgtc cgcctgaggg agcgctaccc gtggctcgtc
    10081 gctgaggttg acggcgaggt ggctgggatc gcttacgctg gcccctggaa ggcgcgcaat
    10141 gcttacgatt ggaccgccga gagcacggtt tacgtgtcgc caaggcacca gaggaccggc
    10201 ctcgggtcaa cactgtacac tcatctcctg aagtccctgg aggctcaggg cttcaagagc
    10261 gtggtcgccg ttattggcct gccaaacgac ccttctgtgc ggatgcacga ggccctcggc
    10321 tacgctccac gcgggatgct gagggctgct ggcttcaagc acgggaattg gcatgacgtg
    10381 ggcttctggc agctcgattt ctcgctgccg gtcccgccga ggccggtgct ccccgtcacg
    10441 gagatctagc tcgatcgagc ggccatgcta gagtccgcaa aaatcaccag tctctctcta
    10501 caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga
    10561 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
    10621 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
    10681 gacctactag taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
    10741 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
    10801 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
    10861 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
    10921 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
    10981 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
    11041 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
    11101 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
    11161 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
    11221 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
    11281 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
    11341 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
    11401 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
    11461 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
    11521 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
    11581 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
    11641 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
    11701 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
    11761 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
    11821 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
    11881 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
    11941 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
    12001 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    12061 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    12121 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    12181 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
    12241 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    12301 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    12361 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    12421 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    12481 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    12541 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    12601 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    12661 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    12721 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    12781 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    12841 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
    12901 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
    12961 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
    13021 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    13081 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
    13141 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
    13201 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
    13261 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
    13321 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
    13381 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
    13441 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
    13501 ctttgggttc ccaatgtacg tgctatccac aggaaacaga ccttttcgac ctttttcccc
    13561 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
    13621 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
    13681 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
    13741 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
    13801 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
    13861 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
    13921 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
    13981 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
    14041 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
    14101 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
    14161 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
    14221 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
    14281 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
    14341 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
    14401 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
    14461 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
    14521 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
    14581 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
    14641 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
    14701 ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca
    14761 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
    14821 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    14881 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    14941 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    15001 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    15061 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    15121 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    15181 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    15241 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    15301 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    15361 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    15421 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    15481 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    15541 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    15601 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    15661 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    15721 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    15781 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    15841 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    15901 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    15961 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    16021 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    16081 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    16141 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    16201 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    16261 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    16321 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    16381 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    16441 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    16501 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    16561 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    16621 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    16681 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    16741 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    16801 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    16861 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    16921 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    16981 cgccctttta aatatccgat tattctaata aacgctcttt tctcttag
//
