LOCUS       sequence-180060-       17520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) 
            under CaMV 35S promoter and empty gRNA scaffold, Basta and 
            glyphosate-resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17520)
  AUTHORS   Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L
  TITLE     CRISPR/Cas9-mediated base-editing system efficiently generates 
            gain-of-function mutations in Arabidopsis.
  JOURNAL   Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.
  PUBMED    28303459
REFERENCE   2  (bases 1 to 17520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci China 
            Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5."
COMMENT     Sequence Label: pXSE901BG
FEATURES             Location/Qualifiers
     source          1..17520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             957..1748
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1900..1975
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2589..2933
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3083..3769
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(3818..3841,3842..3844,3845..7918)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5601..5609
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7931..8179
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8192..8212
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        8969..9645
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    11241..11265
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11690..12484
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12571..13159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13060..13079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13313..13330
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13345..13485
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13411..13430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13571..13593
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13829..14023
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14089..15162)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15591..16220)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttcgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct
      301 tcttcttcgt tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct
      361 ttcgttttct tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc
      421 ccaggattag aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca
      481 ttcttaagat atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag
      541 gcccatttat atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac
      601 aatcttcaaa agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta
      661 gagtcgaagt agtgattggg agaccaaccc agtggacata agcctgttcg gttcgtaagc
      721 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      781 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
      841 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      901 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
      961 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     1021 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     1081 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     1141 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     1201 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1261 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     1321 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1381 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     1441 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     1501 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     1561 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     1621 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     1681 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     1741 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     1801 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     1861 cttttattat ttttaagcgt gcataataag ccggtctcgg ttttagagct agaaatagca
     1921 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     1981 tttgcaaaat tttccagatc gatttcttct tcctctgttc ttcggcgttc aatttctggg
     2041 gttttctctt cgttttctgt aactgaaacc taaaatttga cctaaaaaaa atctcaaata
     2101 atatgattca gtggttttgt acttttcagt tagttgagtt ttgcagttcc gatgagataa
     2161 accaataagc ttgcatgcct gcaggtcaac atggtggagc acgacacact tgtctactcc
     2221 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg
     2281 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
     2341 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
     2401 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     2461 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgataacatg
     2521 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     2581 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     2641 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     2701 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     2761 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     2821 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     2881 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacctcgacc
     2941 tcaacacaac atatacaaaa caaacgaatc tcaagcaatc aagcattcta cttctattgc
     3001 agcaatttaa atcatttctt ttaaagcaaa agcaattttc tgaaaatttt caccatttac
     3061 gaacgatact cgagtaatct agatgtcttc cgagacagga ccggttgccg tcgaccctac
     3121 tcttagaagg cgcattgagc cacacgagtt cgaggtgttc ttcgatccga gagagctgag
     3181 gaaggagact tgcctccttt acgagatcaa ttggggcgga aggcactcta tttggcgcca
     3241 tacctcccag aacacaaata agcatgtgga ggttaatttc atcgagaagt tcactaccga
     3301 gaggtacttc tgcccaaaca cacgctgcag catcacttgg ttccttagct ggtcaccgtg
     3361 cggagagtgc tctcgcgcca ttacagagtt cctgtccaga tacccgcacg ttactctttt
     3421 catctacatt gccagactgt accaccatgc ggatcctcgc aacagacagg gtcttaggga
     3481 cctgatcagc tcaggcgtca ccatccagat tatgacagag caggagtctg gatactgctg
     3541 gcgcaacttc gtgaattact ctccttccaa tgaggctcac tggccaagat acccgcatct
     3601 gtgggtcagg ctctacgtgc tcgagcttta ctgcatcatt cttggtctgc ctccatgcct
     3661 caacatcctt agaaggaagc agccacagct cacattcttc actattgccc ttcagtcttg
     3721 ccactaccag aggcttccgc ctcatattct gtgggcgact ggcctcaaga gcggctcaga
     3781 gactccggga acatctgagt ccgctactcc tgagtctgac aagaagtact ccatcggact
     3841 cgccattggt actaactccg ttggatgggc ggtcatcacc gatgagtaca aggtgcctag
     3901 caagaagttc aaggttcttg gtaacacaga cagacactca atcaagaaga atctgattgg
     3961 tgctctgctc ttcgattctg gagagactgc cgaggctacc aggctcaaga gaaccgcccg
     4021 cagaaggtac acacgcagaa agaataggat ctgctacctt caggagattt tctctaacga
     4081 gatggctaag gttgatgaca gcttcttcca tcgccttgag gagtcattcc tggtcgagga
     4141 ggacaagaag cacgagagac atcctatctt cggtaacatt gtcgatgagg tggcctacca
     4201 cgagaagtac ccaactatct accatcttag gaagaagctg gtggatagca ccgacaaggc
     4261 ggatctccgc cttatctacc tggctctcgc ccacatgatt aagttcagag gccatttcct
     4321 catcgagggc gatctcaacc cagataattc agacgtcgat aagctcttca tccagcttgt
     4381 gcagacatac aatcagcttt tcgaggagaa cccgattaat gcgagcggtg ttgatgcgaa
     4441 ggctatcctg tcagctagac tcagcaagtc aaggcgcctg gagaacctca tcgcccagct
     4501 gccaggcgag aagaagaacg gtcttttcgg caatctgatt gcgctttctc tgggactcac
     4561 cccgaacttc aagtccaatt tcgacctggc tgaggatgcc aagctccagc tgtctaagga
     4621 tacatacgat gacgatctcg acaaccttct ggctcagatc ggcgaccagt acgccgatct
     4681 cttccttgct gccaagaatc ttagcgatgc catcctcctt tcagacattc tgagagttaa
     4741 cactgagatt accaaggctc cgctgtctgc ctccatgatc aagagatacg atgagcacca
     4801 tcaggacctc actctgctca aggcgctggt ccgccagcag ctccctgaga agtacaagga
     4861 gatcttcttc gaccagtcta agaacggcta cgcgggttac attgatggtg gcgctagcca
     4921 ggaggagttc tacaagttca tcaagccaat tctggagaag atggatggca ctgaggagct
     4981 tctggtcaag ctcaataggg aggatctcct taggaagcag cgcaccttcg acaacggatc
     5041 tatccctcac cagattcatc ttggagagct gcacgccatc ctcagaaggc aggaggattt
     5101 ctacccattc cttaaggaca accgcgagaa gatcgagaag attctgactt tcagaatccc
     5161 ttactacgtt ggcccgctcg ctagaggcaa ctctaggttc gcgtggatga ccaggaagtc
     5221 agaggagact atcacccctt ggaacttcga ggaggtggtt gacaagggag ccagcgcgca
     5281 gtcattcatt gagcgcatga ctaatttcga taagaacctg cctaatgaga aggtcctccc
     5341 aaagcatagc ctgctctacg agtacttcac tgtgtacaac gagcttacca aggtgaagta
     5401 tgtgacagag ggcatgcgca agccggcttt cctttcagga gagcagaaga aggccatcgt
     5461 ggatcttctg ttcaagacta atagaaaggt caccgtgaag cagctgaagg aggattactt
     5521 caagaagatt gagtgcttcg actctgttga gatctccggt gtcgaggata ggttcaacgc
     5581 ttccctcggc acctaccacg acctccttaa gatcattaag gacaaggatt tcctggataa
     5641 cgaggagaat gaggacatcc tcgaggatat tgtgctgaca ctcactcttt tcgaggacag
     5701 ggagatgatc gaggagcgcc ttaagacata cgcgcatctg ttcgacgata aggttatgaa
     5761 gcagctcaag cgcagaaggt acactggatg gggtagactc tctaggaagc tcatcaacgg
     5821 catcagagat aagcagtctg gcaagactat tctcgatttc cttaagtccg acggcttcgc
     5881 taacaggaat ttcatgcagc tcattcacga cgattctctt actttcaagg aggacatcca
     5941 gaaggcgcag gttagcggcc agggagattc actgcacgag catatcgcga acctcgctgg
     6001 ctcccctgct atcaagaagg gcatcctcca gaccgttaag gtcgtggatg agctggttaa
     6061 ggtcatgggc agacataagc cagagaacat cgtcattgag atggccaggg agaatcagac
     6121 aactcagaag ggacagaaga actctaggga gcgcatgaag agaatcgagg agggtattaa
     6181 ggagcttggc tcccagatcc tgaaggagca cccggtggag aacacacagc tgcagaatga
     6241 gaagctgtac ctctactacc tccagaatgg ccgcgacatg tatgtggatc aggagcttga
     6301 cattaacaga ctttctgact acgatgtgga ccatatcgtt ccacagtctt tccttaagga
     6361 cgattccatt gataataagg tgctgactag atccgataag aacaggggaa agtctgacaa
     6421 tgttccgtcc gaggaggttg tcaagaagat gaagaactac tggaggcagc tgctcaatgc
     6481 taagctcatc acccagagga agttcgacaa ccttacaaag gccgagcgcg gaggtctgag
     6541 cgagcttgat aaggcgggtt tcattaagag acagctcgtt gagacaaggc agatcactaa
     6601 gcacgtcgcc cagattcttg actcaaggat gaacaccaag tacgacgaga atgataagct
     6661 gatccgcgag gtgaaggtta ttacactgaa gagcaagctc gtttcagatt tcagaaagga
     6721 cttccagttc tacaaggtca gggagatcaa caattaccac catgcccatg atgcgtacct
     6781 caacgcggtg gttggtactg ctcttattaa gaagtacccg aagctggagt ctgagttcgt
     6841 gtacggcgat tacaaggtgt acgacgttag aaagatgatc gctaagagcg agcaggagat
     6901 tggcaaggct accgccaagt acttcttcta ctcaaacatt atgaatttct tcaagacaga
     6961 gatcactctc gcgaacggcg agatcagaaa gaggccactt attgagacta acggcgagac
     7021 aggagagatc gtctgggata agggtcgcga cttcgctact gtcagaaagg tgctctctat
     7081 gccgcaggtt aatattgtca agaagactga ggtgcagacc ggcggattct ctaaggagtc
     7141 cattctccct aagaggaact ccgacaagct catcgcccgc aagaaggatt gggaccctaa
     7201 gaagtacggt ggcttcgata gcccaaccgt cgcttactca gtgcttgtcg tggccaaggt
     7261 cgagaaggga aagagcaaga agctgaagtc agtgaaggag cttctgggta tcacaattat
     7321 ggagaggtct tccttcgaga agaatcctat cgacttcctc gaggcgaagg gctacaagga
     7381 ggttaagaag gatcttatca ttaagctgcc aaagtactca cttttcgagc tggagaacgg
     7441 acgcaagaga atgctggcgt ctgctggaga gcttcagaag ggtaatgagc ttgctctgcc
     7501 gtctaagtat gtgaacttcc tctaccttgc ctctcattac gagaagctca agggctcccc
     7561 tgaggacaac gagcagaagc agctgttcgt cgagcagcac aagcattacc tcgatgagat
     7621 cattgagcag attagcgagt tctcaaagag agtgatcctc gccgatgcga atctcgacaa
     7681 ggttcttagc gcgtacaaca agcaccgcga taagccaatc agagagcagg ctgagaatat
     7741 cattcatctc ttcaccctta caaacctggg tgctccggcg gctttcaagt acttcgatac
     7801 cacaattgac aggaagcgct acacttcaac caaggaggtg ctggacgcca ccctcatcca
     7861 ccagtctatt actggcctct acgagactag gatcgatctc tcccagcttg gtggtgactc
     7921 tggcggatcc accaacctca gcgatatcat tgagaaggag acaggcaagc agcttgttat
     7981 ccaggagtca attctgatgc tcccggagga ggtggaggag gttattggca ataagcctga
     8041 gtctgatatc ctcgtgcata ctgcctacga tgagagcacc gacgagaacg ttatgctcct
     8101 tacatcagac gcgcctgagt acaagccttg ggctctcgtc attcaggatt ccaacggaga
     8161 gaataagatc aagatgctta gcggtggctc tcctaagaag aagagaaagg tgtgagctca
     8221 gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca
     8281 ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt
     8341 cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa
     8401 ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct
     8461 caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga
     8521 gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa
     8581 gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa
     8641 atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg
     8701 ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat
     8761 cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt
     8821 aatgcatttt atgacttgcc aattgattga caacgaattc gaggcggttt gcgtattggc
     8881 tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
     8941 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
     9001 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
     9061 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
     9121 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
     9181 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact
     9241 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
     9301 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
     9361 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
     9421 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     9481 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     9541 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     9601 tcctctatat aaggaagttc atttcatttg gagaggacct cgacaccatc ttccacacac
     9661 tcaagccaca ctattggaga acacacaggg acaacacacc ataagatcca agggaggcca
     9721 ccgccgccgc gccggtaacc accccgcccc tctcctcttt ctttctccgt ttgatctttc
     9781 tttcttcttt ttgtgggtag aatttgaatc cctcagcatt gttcatcggt agtttttctt
     9841 ttcatgattt gtgacaaatg cagcctcgtg cggagctttt ttgtaggtag aagctagatg
     9901 agccccgaga ggaggccagc ggacattagg agggcgactg aggcggatat gccagccgtc
     9961 tgcaccatcg tcaatcacta catcgaaacc tccacggtga acttcaggac agagccgcag
    10021 gagccccagg agtggactga cgatctcgtc cgcctgaggg agcgctaccc gtggctcgtc
    10081 gctgaggttg acggcgaggt ggctgggatc gcttacgctg gcccctggaa ggcgcgcaat
    10141 gcttacgatt ggaccgccga gagcacggtt tacgtgtcgc caaggcacca gaggaccggc
    10201 ctcgggtcaa cactgtacac tcatctcctg aagtccctgg aggctcaggg cttcaagagc
    10261 gtggtcgccg ttattggcct gccaaacgac ccttctgtgc ggatgcacga ggccctcggc
    10321 tacgctccac gcgggatgct gagggctgct ggcttcaagc acgggaattg gcatgacgtg
    10381 ggcttctggc agctcgattt ctcgctgccg gtcccgccga ggccggtgct ccccgtcacg
    10441 gagatcctga acttcgacct cctgaagctg gccggcgatg tcgagtctaa cccagggcct
    10501 atgatcgagg ttaagccgat taatgcggag gacacctacg atctccgcca cagggtcctg
    10561 cgcccaaatc agcctatcga ggcgtgcatg ttcgagtctg acctcacgcg ctcagcgttc
    10621 catctgggcg ggttctacgg cgggaagctc atttcagtgg cgtccttcca ccaggctgag
    10681 catagcgagc tgcagggcaa gaagcagtac cagctcaggg gcgttgctac actggagggc
    10741 taccgcgagc agaaggctgg ctccagcctc gtcaagcacg ctgaggagat cctgcggaag
    10801 cgcggcgcgg atatgatttg gtgcaacgct cggacctcgg cctctggcta ctaccgcaag
    10861 ctcggcttct cggagcaggg ggaggtcttc gacacccccc cagttggccc ccatattctg
    10921 atgtacaagc ggattactta gctcgatcga gcggccatgc tagagtccgc aaaaatcacc
    10981 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc
    11041 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
    11101 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
    11161 ccaaaatcca gtgacctact agtaattcag tacattaaaa acgtccgcaa tgtgttatta
    11221 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
    11281 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
    11341 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
    11401 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
    11461 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg
    11521 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
    11581 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
    11641 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
    11701 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
    11761 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
    11821 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
    11881 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
    11941 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
    12001 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
    12061 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
    12121 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
    12181 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
    12241 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
    12301 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
    12361 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
    12421 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
    12481 ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    12541 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    12601 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    12661 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    12721 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    12781 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    12841 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    12901 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    12961 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    13021 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    13081 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    13141 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    13201 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    13261 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    13321 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
    13381 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    13441 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
    13501 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
    13561 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
    13621 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
    13681 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
    13741 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
    13801 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
    13861 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
    13921 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
    13981 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaca gaccttttcg
    14041 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
    14101 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
    14161 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
    14221 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
    14281 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
    14341 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
    14401 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
    14461 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
    14521 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
    14581 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
    14641 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
    14701 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
    14761 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
    14821 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
    14881 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
    14941 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
    15001 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
    15061 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
    15121 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
    15181 ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg
    15241 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    15301 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    15361 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    15421 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    15481 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    15541 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    15601 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    15661 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    15721 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    15781 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    15841 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    15901 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    15961 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    16021 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    16081 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    16141 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    16201 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    16261 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    16321 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    16381 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    16441 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    16501 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    16561 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    16621 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    16681 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    16741 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    16801 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    16861 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    16921 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    16981 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    17041 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    17101 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    17161 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    17221 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    17281 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    17341 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    17401 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    17461 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
//
