LOCUS       sequence-180080-        1267 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1267)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUDT17 (NUDT17A-c014)
FEATURES             Location/Qualifiers
     source          1..1267
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..206
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             213..233
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccatgggcca ccatcatcat catcattctt ctggtgtgga taacaagttc aacaaggagc
       61 gtcgaagagc tcgccgtgaa attcgccatc tgccgaacct gaaccgcgaa cagcgtcgcg
      121 catttattcg cagcctgcgc gatgatccga gccagagcgc gaacctgctg gcggaagcga
      181 agaagctgaa cgatgcgcag ccgaagggta cagagaacct gtacttccaa tccatggctg
      241 aagtgcgtgt tcaactgctg ctgtcccgtc gcccggaaag cgtgtcgttt gctcgtagcg
      301 tttgtggtct gctgggtgcg ggcccgggtc tgggcacctg gccgattcat tgcagcctga
      361 aacgtggtcg cctggttctg agctctcgtc cgtttccggg cgcatctgct cgtctgccgc
      421 tgcaacgtcc gccgttttgt ccgttcgcag cactggaaga acgtccgcgt gtcccgggtg
      481 cagaactgcc gaccgatcgt ggtgtggacc tgggcgtggc agttatcctg cagagttccg
      541 ataaaaccgt gctgctgacg cgtcgcgcac gtacgctgtc agtctcgccg aacctgtggg
      601 tgccgccggg cggtcatgtt gaactggaag aagaactgct ggacggcggt ctgcgcgaac
      661 tgtgggaaga atcaggtctg cacctgccgc aaggccagtt ctcgtgggtt ccgctgggtc
      721 tgtgggaaag tgcatatccg ccgcgtctgt cctggggcct gccgaaatat catcacattg
      781 tgctgtacct gctggttatc agccaggaat ctcagcaaca gctgcaagca cgcattcagc
      841 cgaacccgaa tgaagttagt gctctgatgt ggctgacccc ggatgttgca gctgcagtcg
      901 cagcagctga agacggtacc gaaacgccgg gtctgctgcc gcaggatctg ccgccgagcg
      961 tcctggcagt ggaactggaa gaagacggtc gtgcccgccc gctggttctg catatgagta
     1021 ccctgctgcg tatgatcccg acgatggcag aagataaaga acgcgtctcc accggtacga
     1081 aatttgctct gaaactgtgg ctgcaacacc tgggtcgtac cccgccgccg tgcaaatctg
     1141 cagcatacct ggatccgggt ccggcaaaag aagaatggaa catggacccg ctgccgccga
     1201 atcagggtag cggcaaatga cagtaaaggt ggatacggat ccgaattcga gctccgtcga
     1261 caagctt
//
