LOCUS       sequence-180082-        1725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1725)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PKD2 (PKD2A-c034)
FEATURES             Location/Qualifiers
     source          1..1725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1642..1662
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1663..1692
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1693..1716
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttaagaagga gatatactat gccccgagtg gcctgggcgg agaggctggt tcgcgggctg
       61 cgaggtctct ggggaacaag actcatggag gaaagcagca ctaaccgaga gaaatacctt
      121 aaaagtgttt tacgggaact ggtcacatac ctcctttttc tcatagtctt gtgcatcttg
      181 acctacggca tgatgagctc caatgtgtac tactacaccc ggatgatgtc acagctcttc
      241 ctagacaccc ccgtgtccaa aacggagaaa actaacttta aaactctgtc ttccatggaa
      301 gacttctgga agttcacaga aggctcctta ttggatgggc tgtactggaa gatgcagccc
      361 agcaaccaga ctgaagctga caaccgaagt ttcatcttct atgagaacct gctgttaggg
      421 gttccacgaa tacggcaact ccgagtcaga aatggatcct gctctatccc ccaggacttg
      481 agagatgaaa ttaaagagtg ctatgatgtc tactctgtca gtagtgaaga tagggctccc
      541 tttgggcccc gaaatggaac cgcttggatc tacacaagtg aaaaagactt gaatggtagt
      601 agccactggg gaatcattgc aacttatagt ggagctggct attatctgga tttgtcaaga
      661 acaagagagg aaacagctgc acaagttgct agcctcaaga aaaatgtctg gctggaccga
      721 ggaaccaggg caacttttat tgacttctca gtgtacaacg ccaacattaa cctgttctgt
      781 gtggtcaggt tattggttga attcccagca acaggtggtg tgattccatc ttggcaattt
      841 cagcctttaa agctgatccg atatgtcaca acttttgatt tcttcctggc agcctgtgag
      901 attatctttt gtttctttat cttttactat gtggtggaag agatattgga aattcgcatt
      961 cacaaactac actatttcag gagtttctgg aattgtctgg atgttgtgat cgttgtgctg
     1021 tcagtggtag ctataggaat taacatatac agaacatcaa atgtggaggt gctactacag
     1081 tttctggaag atcaaaatac tttccccaac tttgagcatc tggcatattg gcagatacag
     1141 ttcaacaata tagctgctgt cacagtattt tttgtctgga ttaagctctt caaattcatc
     1201 aattttaaca ggaccatgag ccagctctcg acaaccatgt ctcgatgtgc caaagacctg
     1261 tttggctttg ctattatgtt cttcattatt ttcctagcgt atgctcagtt ggcatacctt
     1321 gtctttggca ctcaggtcga tgacttcagt actttccaag agtgtatctt cactcaattc
     1381 cgtatcattt tgggcgatat caactttgca gagattgagg aagctaatcg agttttggga
     1441 ccaatttatt tcactacatt tgtgttcttt atgttcttca ttcttttgaa tatgtttttg
     1501 gctatcatca atgatactta ctctgaagtg aaatctgact tggcacagca gaaagctgaa
     1561 atggaactct cagatcttat cagaaagggc taccataaag ctttggtcaa actaaaactg
     1621 aaaaaaaata ccgtggatgc agagaacctc tacttccaat cgcaccatca tcaccatcac
     1681 catcaccacc atgattacaa ggatgacgac gataagtgag gatcc
//
