LOCUS       sequence-180087-        1648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1648)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RECQL5 (RECQL5A-c011)
FEATURES             Location/Qualifiers
     source          1..1648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggaccctga gcggcgagtc cggagtacgc tgaagaaggt ctttgggttt
      121 gactctttta agacgccttt acaggagagt gcgaccatgg ctgtagtaaa aggtaacaag
      181 gacgtctttg tgtgcatgcc cacaggggca ggaaaatccc tatgctatca gctccctgct
      241 ctgttggcca aaggcatcac cattgtagtc tctcctctca ttgctttgat tcaggaccaa
      301 gtggaccact tgctaaccct aaaggtacga gtaagttccc tgaactcgaa gctctctgca
      361 caggaaagga aggagctgct tgctgacctg gagcgagaaa agccccagac caagattctg
      421 tacatcaccc cagagatggc agcttcatcc tccttccagc ccaccctgaa ctccctggtg
      481 tcccgccacc tgctgtctta cttggtggtg gatgaagctc attgtgtttc ccaatggggg
      541 catgactttc gtcctgacta cttgcgtctg ggtgccctgc gctcccgcct gggacatgcc
      601 ccttgtgtgg ctctgaccgc cacagccacc ccacaggtcc aagaggacgt gtttgctgcc
      661 ctgcacctga agaaaccagt tgccatcttc aagactccct gcttccgggc caacctcttc
      721 tatgatgtgc aattcaagga actgatttct gatccctatg ggaacctgaa ggacttctgc
      781 cttaaggctc ttggacagga ggctgataaa gggttatctg gctgcggcat tgtgtactgc
      841 aggactagag aggcttgtga acagctggcc atagagctca gctgcagggg tgtgaacgcc
      901 aaggcttacc atgcagggct gaaggcctct gaaagaacgc tggtgcagaa cgactggatg
      961 gaggagaagg tccctgtaat tgttgcaacc attagttttg ggatgggagt ggataaagcc
     1021 aatgtcaggt ttgtcgccca ttggaatatt gccaagtcta tggctgggta ctaccaggag
     1081 tctggccggg ctggcaggga tgggaagcct tcctggtgcc gtctctatta ctccaggaat
     1141 gaccgggacc aagtcagctt cctgatcagg aaggaagtag caaaactcca ggaaaagaga
     1201 ggaaacaaag catctgataa agccactatc atggcctttg atgccctggt gaccttctgt
     1261 gaagaactgg ggtgccgcca tgccgccatt gccaagtact tcggggatgc gctgcctgcc
     1321 tgcgccaaag gctgcgacca ctgccagaac cccacggccg tgcggaggcg gctggaggcc
     1381 ttggagcgca gcagcagctg gagcaagacc tgcatcgggc cctcccaggg gaacggcttt
     1441 gaccccgagc tgtacgaggg aggccgcaag ggctacgggg acttcagcag gtatgacgaa
     1501 ggttctggag gcagcgggga tgaaggcaga gatgaggccc acaagcggga gtggaacctc
     1561 ttctatcaga agcagatgca gctgcgcaag ggcaaagacc ccaagataga agaatttgta
     1621 tgacagtaaa ggtggatacg gatccgaa
//
