LOCUS       sequence-180088-         361 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 361)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WNK1 (WNK1A-c011)
FEATURES             Location/Qualifiers
     source          1..361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgacttcacg cccaaaatta agaattttga atgtttcaaa taaaggagac
      121 cgagtagtag aatgtcaatt agagactcat aataggaaaa tggttacatt caaatttgac
      181 ctagatggtg acaaccccga ggagatagca acaattatgg tgaacaatga ctttattcta
      241 gcaatagaga gagagtcgtt tgtggatcaa gtgcgagaaa ttattgaaaa agctgatgaa
      301 atgctcagtg aggatgtcag tgtggaacca gagtgacagt aaaggtggat acggatccga
      361 a
//
