LOCUS       sequence-180089-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KLHL40 (KBTBD5A-c006)
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             947..967
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             968..985
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             986..1009
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tgttcctgca ggatctcatc ttcatgatca gtgaggaggg
       61 cgctgtggcc tacgatccag cagccaacga gtgctactgt gcttccctct ccagccaggt
      121 ccccaagaac cacgtcagcc tggttaccaa ggagaaccag gtcttcgtgg ctggaggcct
      181 cttctacaac gaagacaaca aagaggaccc catgagcgca tacttcctgc agtttgacca
      241 tctggactca gagtggctgg ggatgccacc gctgccctcg ccccgctgcc tctttggcct
      301 gggagaagct ctcaactcca tctacgtggt cggtggcaga gagatcaagg acggcgagcg
      361 ctgcctggac tcggtcatgt gctacgacag gctgtcattc aaatggggtg aatcggaccc
      421 gctgccttac gtggtgtatg gccacacagt gctctcccac atggaccttg tctacgtaat
      481 tggcggcaaa ggcagtgaca ggaagtgcct gaacaagatg tgcgtctatg accccaagaa
      541 gtttgagtgg aaggagctgg cacccatgca gaccgcccgc tcactctttg gggccactgt
      601 ccatgatggc cgcattatcg tggcagctgg ggtcaccgac acagggctga ccagttctgc
      661 cgaagtgtac agcatcacag acaacaagtg ggcacccttc gaggccttcc cacaggagcg
      721 tagctcactc agcctggtca gcctggtggg taccctctat gccattggtg gctttgccac
      781 actggagacg gagtctggag agctggttcc cacagagctc aatgacatct ggaggtataa
      841 cgaggaggag aagaaatggg agggtgtcct gcgggagatc gcctatgcag caggtgccac
      901 cttcctacca gtgcggctca atgtgctgcg cctgactaag atggcagaga acctctactt
      961 ccaatcgcac catcatcacc accatgatta caaggatgac gacgataagt gaggatcc
//
