LOCUS       sequence-18009-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Milne JC, Blanke SR, Hanna PC, Collier RJ
  TITLE     Protective antigen-binding domain of anthrax lethal factor mediates 
            translocation of a heterologous protein fused to its amino- or 
            carboxy-terminus.
  JOURNAL   Mol Microbiol. 1995 Feb . 15(4):661-6.
  PUBMED    7783638
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 1995 Feb . 15(4):661-6."
COMMENT     Sequence Label: pET-15b LFN-DTA
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggcgggcgg tcatggtgat
      121 gtaggtatgc acgtaaaaga gaaagagaaa aataaagatg agaataagag aaaagatgaa
      181 gaacgaaata aaacacagga agagcattta aaggaaatca tgaaacacat tgtaaaaata
      241 gaagtaaaag gggaggaagc tgttaaaaaa gaggcagcag aaaagctact tgagaaagta
      301 ccatctgatg ttttagagat gtataaagca attggaggaa agatatatat tgtggatggt
      361 gatattacaa aacatatatc tttagaagca ttatctgaag ataagaaaaa aataaaagac
      421 atttatggga aagatgcttt attacatgaa cattatgtat atgcaaaaga aggatatgaa
      481 cccgtacttg taatccaatc ttcggaagat tatgtagaaa atactgaaaa ggcactgaac
      541 gtttattatg aaataggtaa gatattatca agggatattt taagtaaaat taatcaacca
      601 tatcagaaat ttttagatgt attaaatacc attaaaaatg catctgattc agatggacaa
      661 gatcttttat ttactaatca gcttaaggaa catcccacag acttttctgt agaattcttg
      721 gaacaaaata gcaatgaggt acaagaagta tttgcgaaag cttttgcata ttatatcgag
      781 ccacagc
//
