LOCUS       sequence-180093-         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CFL1 (CFL1A-c002)
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggcctgcgg tgtggctgtc tctgatggtg tcatcaaggt gttcaacgac
      121 atgaaggtgc gtaagtcttc aacgccagag gaggtgaaga agcgcaagaa ggcggtgctc
      181 ttctgcctga gtgaggacaa gaagaacatc atcctggagg agggcaagga gatcctggtg
      241 ggcgatgtgg gccagactgt cgacgatccc tacgccacct ttgtcaagat gctgccagat
      301 aaggactgcc gctatgccct ctatgatgca acctatgaga ccaaggagag caagaaggag
      361 gatctggtgt ttatcttctg ggcccccgag tctgcgcccc ttaagagcaa aatgatttat
      421 gccagctcca aggacgccat caagaagaag ctgacaggga tcaagcatga attgcaagca
      481 aactgctacg aggaggtcaa ggaccgctgc accctggcag agaagctggg gggcagtgcc
      541 gtcatctccc tggagggcaa gcctttgtga cagtaaaggt ggatacggat ccgaa
//
