LOCUS       sequence-180094-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPIN2B (SPIN2BA-c011)
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             689..706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             707..730
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttaagaagg agatatacta tgccccgcag gaacatcgtg ggctgcagaa tttctcatgg
       61 atggaaggaa ggagatgagc ccatcacgca gtggaaagga accgttctgg atcaggtgcc
      121 tataaatccc tctctttatc tggtgaaata tgatggaatt gactgtgtct atggactgga
      181 acttcacaga gatgaaaggg ttttgtctct taaaattctt tctgacaggg tggcatcatc
      241 tcacattagt gatgccaacc ttgcaaatac cataattggc aaagcagtgg aacatatgtt
      301 tgagggagag catggttcta aggatgaatg gagggggatg gtcttagctc aagcacctat
      361 catgaaagcc tggttttata ttacctatga gaaagatcct gtcttgtaca tgtaccagct
      421 tctagatgat tataaggaag gtgacctccg catcatgcca gaatccagtg agtctcctcc
      481 aacagagagg gagccaggag gagttgtaga tggcctaata ggtaagcatg tggaatatac
      541 caaagaagat ggctccaaaa ggatcggcat ggtcattcac caagtggaag ccaaaccctc
      601 tgtgtatttc atcaagtttg atgatgattt ccatatctat gtctacgatt tggtgaaaaa
      661 gtccgcagag aacctctact tccaatcgca ccatcatcac caccatgatt acaaggatga
      721 cgacgataag tgaggatcc
//
