LOCUS       sequence-180099-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TRIM33 (TRIM33B-c100)
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggatgatga cccaaatgaa gactggtgtg ctgtctgcca aaacggagga
      121 gatctcttgt gctgcgaaaa atgtccaaag gtctttcatc taacttgtca tgttccaaca
      181 ctacttagct ttccaagtgg ggactggata tgcacatttt gtagagatat tggaaagcca
      241 gaagttgaat atgattgtga taatttgcaa catagtaaga aggggaaaac tgcgcagggg
      301 ttaagccccg tggaccaaag gaaatgtgaa cgtcttctgc tttacctcta ttgccatgaa
      361 ttaagtattg aattccagga gcctgttcct gcttcgatac caaactacta taaaattata
      421 aagaaaccaa tggatttatc caccgtgaaa aagaagcttc agaaaaaaca ttcccaacac
      481 taccaaatcc cggatgactt tgtggccgat gtccgtttga tcttcaagaa ctgtgaaagg
      541 tttaatgaag ctgattcaga agtagctcag gcagggaaag cagttgcatt gtactttgaa
      601 gataaactca cagagatcta ctcagacagg accttcgcac ctttgccatg acagtaaagg
      661 tggatacgga tccgaa
//
