LOCUS       sequence-180112-        5900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NSII vector expressing PkaiB-KaiBC. Nt resistant. Complements 
            rhthyms in AMC705.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Cohen SE, Erb ML, Selimkhanov J, Dong G, Hasty J, Pogliano J, Golden
            SS
  TITLE     Dynamic localization of the cyanobacterial circadian clock proteins.
  JOURNAL   Curr Biol. 2014 Aug 18;24(16):1836-44. doi: 
            10.1016/j.cub.2014.07.036. Epub 2014 Aug 7.
  PUBMED    25127213
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.07.036"; journalName: "Curr Biol"; date: 
            "2014-08-18- 18"; volume: "24"; issue: "16"; pages: "1836-44"
COMMENT     Sequence Label: pAM4645
FEATURES             Location/Qualifiers
     source          1..5900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1059..1199
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1114..1133
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1214..1231)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1385..1973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1465..1484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctagcat ccgcgcgcgc gcgcgcgatg ctagcggggg tgtgcagttc caagcagctg
       61 cgctagaagc cgaagccgca caggggcaac tcagtcagcg atcgctggaa cagtggtgtc
      121 aggtgttgat ggccggtgca gcggcagagc aactggtcta cggcaacgtg gaagggggag
      181 ctgacgatcg cgcccagtgg aaacaactgt ggcggcaact cgatcgcaat cctgccgaag
      241 cggatttacg cagtcgctgg ggattgttac gggcgaagac tttactggag caacaacgtc
      301 ccgcctacga tgctttggtg gcggcgatgg ctgcagaggc cagcattgaa gactgcaatc
      361 aagcgatcgc cactgcttgg gtagaagaac ctgcgatcgc cgcttagtga agagtccaga
      421 agattcccct cccctctcgc ccaatggaca agggaaatgt gattcagcat gagtcaagtc
      481 cccagtgcat ggatgggtgt gcaatgacgg acagaggctg ttgactctca gttatccctg
      541 taagccaaat cgtccgaaaa tcaccagctg aaacggttag ggttgcattg tttttaagca
      601 cgagccatca acagtaagag cgatcgcgca ggaacggttt agtgtgtagt tgcggcgcga
      661 gcaggggtta gggattgggc aatgctgtgg agttatcttt tggcgacgat tggagcagac
      721 tgggcagcag tcgcatatcg tcaaaaatga gatgggcacc ggcttcttgc agacgccaag
      781 cgggcatgga acccgcgtag ccaaacactt gcattcctgc cgccacgcct gccgctactc
      841 ccaagggggt gtcttcgatc acagcgcagg ccgtaggatt aaccccgaag cgatcggcgg
      901 ccaacaaaaa gacatcggga aatggcttgc cgcgaggtac ttcagtcacg ctgaagattc
      961 gtccctcaaa tcgtggcccg cggagcttgt ctgtaagcgg atggcgggag cagacaagcc
     1021 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     1081 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     1141 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     1201 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1261 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1321 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1381 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1441 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1501 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1561 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1621 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1681 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1741 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1801 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     1861 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1921 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1981 ctttgatctt ttctacgggg tctgacgcta tcaggattaa tgaaacggac gccccagact
     2041 gaattgccgg actgggaagc gatcgcggat ttggatgcga tcgtggtcga taaacgagct
     2101 cgtaagcggg ccacggcagc gaaagggcga cggcgcgatc gccggtatgg aaaacgcttg
     2161 ctgcagcatc agatcgatgc gatcgccgaa gactgtgacc tagatgagga agcatgagcg
     2221 aactgggctt gagtctgacg gcgatcgcga tttttaccac gacggcatta gctttggtgg
     2281 gaccaatgct gggtagttct ccgctgctac cggcgggatt gggttttagc ctcttggtgc
     2341 tgttcagtct ggatgcggtg acttggcagg ggcggggtgc cacgttactg ctcgatggca
     2401 ttcagcagcg atcgcccgaa tatcgtcagc ggattttgca tcacgaagcg ggtcactact
     2461 tggtagcaac tctagaatcc ccccgggggc ccccgactcg ctcagcgact acaagagcgc
     2521 ctaggctatc tcggggtcta ctacaagcgt gatcccgatc gctttctgcg caacctaccc
     2581 gcctacgaaa gccaaaagct gcaccaagcg atgcagacta gctatcgtga aatcgttttg
     2641 agctattttt cgccgaatag caacctcaac cagagcattg acaacttcgt caacatggct
     2701 ttctttgccg atgttccagt caccaaagtg gtagaaattc acatggagct gatggacgag
     2761 tttgccaaga agctccgcgt agagggacgt tcagaggaca ttttgctgga ttatcggctg
     2821 actttaattg atgtaattgc acatctttgt gagatgtatc gacggtctat cccacgagaa
     2881 acctgaaaag gtaaaggagg tcttaagctc ggctcaattt ctctctttat cctgttagat
     2941 ggtttgattg ctgttgctac cccgttgatc tgcgtatgag ccctcgtaaa acctacattc
     3001 tcaagctcta cgtcgccggc aatactccaa actcagtccg tgccctcaaa acgctcaaga
     3061 acattctcga agttgaattt caaggtgttt atgctctaaa ggtgatcgat gttctcaaaa
     3121 atcctcagtt ggcagaagag gataaaatcc tagcgacgcc aaccctcgcc aaggttctac
     3181 cactgcctgt ccgacggatt attggtgatt tatccgaccg tgagaaagtt ttgattggcc
     3241 ttgatttact ctacggcgaa cttcaagatt ccgacgactt ctaactgtcg ttaactgctt
     3301 tgttggtact acctgacttc accctctttt aagatgactt ccgctgagat gactagccct
     3361 aataataatt ctgagcacca agccatcgct aagatgcgca cgatgattga aggctttgat
     3421 gatattagtc atggcggtct tccaatcggg cgatcgaccc tcgttagtgg tacttcagga
     3481 accggcaaga cccttttttc tattcaattt ctctataacg gtattatcga gtttgatgag
     3541 cctggggttt tcgttacttt cgaagaaacc ccgcaagata tcattaaaaa cgcccgtagt
     3601 tttggctggg atttagccaa gctggtcgat gagggcaaac tatttattct tgatgcttca
     3661 cccgatccag aaggtcaaga ggttgttggc ggcttcgatc tctctgctct gattgagcgg
     3721 attaattatg caattcaaaa gtatcgagcg cggcgggttt caattgactc ggtcacgtcc
     3781 gttttccagc aatatgatgc ctcttctgtg gttcgccgcg aactctttcg gttggtagct
     3841 cgcctaaaac aaattggggc aactacggtc atgaccaccg agcgtatcga ggaatatggc
     3901 ccgatcgctc gttacggtgt tgaggaattt gtctccgata acgtcgtgat tctccgcaac
     3961 gttttggaag gggagcgccg tcgccgcacc ctcgaaatcc tcaagctacg tggcaccagc
     4021 cacatgaaag gggaatatcc gttcacgatt acggatcatg gcatcaatat cttcccgctc
     4081 ggggcaatgc gccttacgca gcgatcgtcg aacgtgcgtg tttcatctgg tgtcgtccga
     4141 ctcgatgaaa tgtgtggtgg gggcttcttt aaggactcaa tcattctggc aactggcgct
     4201 acaggcactg gtaaaactct gttagttagc cgtttcgttg agaatgcttg tgctaacaaa
     4261 gagcgggcga ttctgttcgc ttatgaagag tcacgagctc agctgctccg caatgcctat
     4321 tcatggggaa tggactttga ggagatggag cgccaaaacc tcctcaaaat tgtttgcgcc
     4381 tatcctgaat ctgcaggtct tgaagaccat ttgcagatta ttaagtcgga gatcaatgac
     4441 tttaagccag ctcgtattgc aatcgactcc ctctctgctt tggcgcgggg cgttagcaac
     4501 aatgccttcc gccaatttgt aattggtgtc actggctacg cgaaacaaga agaaatcacg
     4561 ggactattca caaataccag tgatcaattt atgggagcgc attcgattac tgactcccat
     4621 atctcaacaa ttacggatac gattatcttg ctccaatacg tcgagattcg tggcgaaatg
     4681 tcccgcgcca ttaacgtctt caagatgcgc ggatcttggc atgacaaagc aatccgcgaa
     4741 ttcatgatca gcgacaaagg gccggacatc aaggattctt tccggaactt tgagcggatt
     4801 atttcaggtt cgccaacacg gattaccgtc gatgagaaaa gcgaactctc gcgaattgtg
     4861 cgcggcgttc aagaaaaagg gccggagagc tagccagcga tcgcgcttag gtattcttgt
     4921 ctcagctcag tcgtcaaccc gagcaccgag aaagctaagg cctcgcgata aagccgttgt
     4981 gctggattct gtaatccggc ggcggcggcg ggataccggt ttcgaagaat tctcatgttt
     5041 gacagcttat catcgaatta taggaataga gcaaacaagc aaaggaaatt ttgtcaaaat
     5101 aattttattg acaacgtctt attaacgttg atataattta aattttattt gacaaaaatg
     5161 ggctcgtgtt gtacaataaa tgtagtgagg tggatgcaat ggcgaagacg ttgtccgata
     5221 ttaaaagatc gcttgatggg aatttaggta aaaggctgac gttaaaagca aacggtggcc
     5281 ggatccatat gaccaccctg gatgataccg cctaccgcta ccgcaccagc gttcccggtg
     5341 atgccgaagc catcgaagcc ctggatggca gctttaccac cgataccgtg tttcgcgtga
     5401 ccgccacggg tgatggcttt accctgcgcg aagtgcccgt cgatccccct ctgaccaaag
     5461 tgtttcccga tgatgaaagt gatgatgaat cggatgctgg cgaagatggc gatcccgata
     5521 gccgcacctt tgtggcctac ggtgatgatg gcgatctggc tggctttgtg gtggtgagct
     5581 acagcggctg gaatcgccgc ttgaccgtgg aagatattga agtggctccc gaacaccgcg
     5641 gtcacggcgt tggtcgcgct ctgatgggcc tggccaccga atttgctcgc gaacgcggtg
     5701 ctggccacct gtggctggaa gtgaccaacg tgaacgctcc cgctatccac gcctatcgcc
     5761 gcatgggctt caccctgtgt ggcctggata ccgctctgta cgatggcacc gccagtgatg
     5821 gcgaacaggc cctgtacatg agcatgccct gcccctaagg ccggccagcc cgcctaatga
     5881 gcgggctttt ttttggcgcg
//
