LOCUS       sequence-180120-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial 
            expression. Insert is human codon optimized but expresses well in 
            bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung 
            J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, 
            Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, 
            Collins JJ, Zhang F
  TITLE     Nucleic acid detection with CRISPR-Cas13a/C2c2.
  JOURNAL   Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.
  PUBMED    28408723
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321."
COMMENT     Sequence Label: pC013 - Twinstrep-SUMO-huLwCas13a
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..110
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(137..142,143..159,160..165)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(340..387)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     383..401
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
       61 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
      121 tcaagaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt agtttatcac
      181 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
      241 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
      301 gcctcttgcg ggatatccgg atatagttcc tcctttcagc aaaaaacccc tcaagacccg
      361 tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag ccaactcagc
      421 ttcctttcgg gcctcgagtg cggccgctta ttccagggcc ttgtactcga acatgacttt
      481 cacgagttcg cacagttcct cgctgttccg gtcggtcatc agtttctttt tcttcagatt
      541 cttcaggtgc acgatcttct ctgattccag ggtctggatt tcgatcttct tgtcagcgcc
      601 gatcttgaag gtggccacga agccgtattc tttcagaatg tccacgatgg acttcatgat
      661 ggcgttcttc agcttccggt cgtaggacag cagcttccgc aggttttcca gcacttccag
      721 caggctaatc tcggcgtggg ggatgtagtt gaagtgggca atgtagttcc ggatgtacag
      781 gtcctttttt tcctgcttca gtttcttcac tttcttgtcg gagtagatgc tccgcttttc
      841 cacattgtcc ttgtacagtt ctttgtaga
//
