LOCUS       sequence-180123-        4383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  KaiB expressed from native promoter from NSII-Nt. Complements 
            rhythms of kaiB null strain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Cohen SE, Erb ML, Selimkhanov J, Dong G, Hasty J, Pogliano J, Golden
            SS
  TITLE     Dynamic localization of the cyanobacterial circadian clock proteins.
  JOURNAL   Curr Biol. 2014 Aug 18;24(16):1836-44. doi: 
            10.1016/j.cub.2014.07.036. Epub 2014 Aug 7.
  PUBMED    25127213
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.07.036"; journalName: "Curr Biol"; date: 
            "2014-08-18- 18"; volume: "24"; issue: "16"; pages: "1836-44"
COMMENT     Sequence Label: pAM5089
FEATURES             Location/Qualifiers
     source          1..4383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      5..593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     494..513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     747..764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    779..919
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(845..864)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      3822..3849
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
       61 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      121 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
      181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      481 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      541 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      601 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      661 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      721 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      781 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
      841 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
      901 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      961 ttgtctgctc ccgccatccg cttacagaca agctccgcgg gccacgattt gagggacgaa
     1021 tcttcagcgt gactgaagta cctcgcggca agccatttcc cgatgtcttt ttgttggccg
     1081 ccgatcgctt cggggttaat cctacggcct gcgctgtgat cgaagacacc cccttgggag
     1141 tagcggcagg cgtggcggca ggaatgcaag tgtttggcta cgcgggttcc atgcccgctt
     1201 ggcgtctgca agaagccggt gcccatctca tttttgacga tatgcgactg ctgcccagtc
     1261 tgctccaatc gtcgccaaaa gataactcca cagcattgcc caatccctaa cccctgctcg
     1321 cgccgcaact acacactaaa ccgttcctgc gcgatcgctc ttactgttga tggctcgtgc
     1381 ttaaaaacaa tgcaacccta accgtttcag ctggtgattt tcggacgatt tggcttacag
     1441 ggataactga gagtcaacag cctctgtccg tcattgcaca cccatccatg cactggggac
     1501 ttgactcatg ctgaatcaca tttcccttgt ccattgggcg agaggggagg ggaatcttct
     1561 ggactcttca ctaagcggcg atcgcaggtt cttctaccca agcagtggcg atcgcttgat
     1621 tgcagtcttc aatgctggcc tctgcagcca tcgccgccac caaagcatcg taggcgggac
     1681 gttgttgctc cagtaaagtc ttcgcccgta acaatcccca gcgactgcgt aaatccgctt
     1741 cggcaggatt gcgatcgagt tgccgccaca gttgtttcca ctgggcgcga tcgtcagctc
     1801 ccccttccac gttgccgtag accagttgct ctgccgctgc accggccatc aacacctgac
     1861 accactgttc cagcgatcgc tgactgagtt gcccctgtgc ggcttcggct tctagcgcag
     1921 ctgcttggaa ctgcacaccc ccgctagcat cgcgcgcgcg cggatgctag cggcgcgcca
     1981 aaaaaaagcc cgctcattag gcgggctggc cggccttagg ggcagggcat gctcatgtac
     2041 agggcctgtt cgccatcact ggcggtgcca tcgtacagag cggtatccag gccacacagg
     2101 gtgaagccca tgcggcgata ggcgtggata gcgggagcgt tcacgttggt cacttccagc
     2161 cacaggtggc cagcaccgcg ttcgcgagca aattcggtgg ccaggcccat cagagcgcga
     2221 ccaacgccgt gaccgcggtg ttcgggagcc acttcaatat cttccacggt caagcggcga
     2281 ttccagccgc tgtagctcac caccacaaag ccagccagat cgccatcatc accgtaggcc
     2341 acaaaggtgc ggctatcggg atcgccatct tcgccagcat ccgattcatc atcactttca
     2401 tcatcgggaa acactttggt cagaggggga tcgacgggca cttcgcgcag ggtaaagcca
     2461 tcacccgtgg cggtcacgcg aaacacggta tcggtggtaa agctgccatc cagggcttcg
     2521 atggcttcgg catcaccggg aacgctggtg cggtagcggt aggcggtatc atccagggtg
     2581 gtcatatgga tccggccacc gtttgctttt aacgtcagcc ttttacctaa attcccatca
     2641 agcgatcttt taatatcgga caacgtcttc gccattgcat ccacctcact acatttattg
     2701 tacaacacga gcccattttt gtcaaataaa ttttaaatta tatcaacgtt aataagacgt
     2761 tgtcaataaa attattttga caaaatttcc tttgcttgtt tgctctattc ctataattcg
     2821 atgataagct gtcaaacatg agaattcttc gaaaccggta tcccgccgcc gccgccggat
     2881 accggttaat agtacctgtg aagtgaaaaa tggcgcacat tgtgcgacat ttttttctcg
     2941 agcaggtagt cggccaataa cccagggatt cgctcagcga ctacaagagc gcctaggcta
     3001 tctcggggtc tactacaagc gtgatcccga tcgctttctg cgcaacctac ccgcctacga
     3061 aagccaaaag ctgcaccaag cgatgcagac tagctatcgt gaaatcgttt tgagctattt
     3121 ttcgccgaat agcaacctca accagagcat tgacaacttc gtcaacatgg ctttctttgc
     3181 cgatgttcca gtcaccaaag tggtagaaat tcacatggag ctgatggacg agtttgccaa
     3241 gaagctccgc gtagagggac gttcagagga cattttgctg gattatcggc tgactttaat
     3301 tgatgtaatt gcacatcttt gtgagatgta tcgacggtct atcccacgag aaacctgaaa
     3361 aggtaaagga ggtcttaagc tcggctcaat ttctctcttt atcctgttag atggtttgat
     3421 tgctgttgct accccgttga tctgcgtatg agccctcgta aaacctacat tctcaagctc
     3481 tacgtcgccg gcaatactcc aaactcagtc cgtgccctca aaacgctcaa gaacattctc
     3541 gaagttgaat ttcaaggtgt ttatgctcta aaggtgatcg atgttctcaa aaatcctcag
     3601 ttggcagaag aggataaaat cctagcgacg ccaaccctcg ccaaggttct accactgcct
     3661 gtccgacgga ttattggtga tttatccgac cgtgagaaag ttttgattgg ccttgattta
     3721 ctctacggcg aacttcaaga ttccgacgac ttctaactgt cgttaactgc tttgttggta
     3781 ctacctgact tcaccctcaa atgaaggagc tccccggcag gagaaggcca tcctgacgga
     3841 tggccttttt gaagctttct agaatcgggg gcccccgggg ggattctaga gttgctacca
     3901 agtagtgacc cgcttcgtga tgcaaaatcc gctgacgata ttcgggcgat cgctgctgaa
     3961 tgccatcgag cagtaacgtg gcaccccgcc cctgccaagt caccgcatcc agactgaaca
     4021 gcaccaagag gctaaaaccc aatcccgccg gtagcagcgg agaactaccc agcattggtc
     4081 ccaccaaagc taatgccgtc gtggtaaaaa tcgcgatcgc cgtcagactc aagcccagtt
     4141 cgctcatgct tcctcatcta ggtcacagtc ttcggcgatc gcatcgatct gatgctgcag
     4201 caagcgtttt ccataccggc gatcgcgccg tcgccctttc gctgccgtgg cccgcttacg
     4261 agctcgttta tcgaccacga tcgcatccaa atccgcgatc gcttcccagt ccggcaattc
     4321 agtctggggc gtccgtttca ttaatcctga tagcgtcaga ccccgtagaa aagatcaaag
     4381 gat
//
