LOCUS       sequence-180132-        9889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mouse PRMT7 with Flag-tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Lee SH, Chen TY, Dhar SS, Gu B, Chen K, Kim YZ, Li W, Lee MG
  TITLE     A feedback loop comprising PRMT7 and miR-24-2 interplays with Oct4, 
            Nanog, Klf4 and c-Myc to regulate stemness.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10603-10618. Epub 2016 Sep 12.
  PUBMED    27625395
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-12-15"; volume: "44(22)"; pages: 
            "10603-10618. Epub 2016 Sep 12"
COMMENT     Sequence Label: pCDH-Flag-mPRMT7-GFP
FEATURES             Location/Qualifiers
     source          1..9889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    95..211
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        254..465
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             478..746
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             783..806
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2898..3470
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3065..3082)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3292..3311
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3503..4165
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    4256..4844
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4309..4329)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4727..4738)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4918..5151
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5223..5344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5260..5279)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5314..5333
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5384..5519
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5552..5568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5552..5568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5565..5587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5576..5592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5600..5606,5607..5624,5625..5630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5645..5666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5783..5800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5954..6542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6713..7504,7505..7573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7336..7355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7574..7678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7746..7764
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7802..7824)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7924..7943
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8137..8159
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8151..8168
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8152..8168
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8183..8409
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8410..8590
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8637..8762
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9255..9488
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9673..9717
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
       61 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
      121 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      181 tacaaaaaca aattacaaaa ttcaaaattt tatcgatact agtggatctg cgatcgctcc
      241 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      301 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      361 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      421 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
      481 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
      541 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
      601 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
      661 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
      721 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctactct agagccacca
      781 tggactacaa agacgatgac gacaagaagg tcttctgtgg ccgtgccaat cctaccacgg
      841 gatccctgga gtggctggag gaggatgaac actatgatta ccaccaggag attgccaggt
      901 catcctatgc cgacatgcta catgacaaag acagaaatat aaaatactac cagggtatcc
      961 gggcagctgt gagcagggtg aaagacagag gacagaaggc cttggttctt gacattggca
     1021 ctggcacagg cctcttgtca atgatggcag ttactgcagg ggctgacttc tgctatgcta
     1081 tcgaggtttt taagcctatg gctgaggctg ctgtgaagat tgtggagagg aatggcttca
     1141 gtgataagat taaagtcatt aacaagcact ccactgaggt gacagtcgga ccagatggtg
     1201 acttgccgtg tcgtgctaac attctgatca cggagctgtt tgacacagag ctgattgggg
     1261 agggagcgct gccctcttat gagcatgcac acaagcatct tgtccaggaa gactgcgagg
     1321 cagtgccaca cagggcaact gtctatgccc agctggtgga gtcccgaagg atgtggtcct
     1381 ggaacaagct gtttcccgtc cgtgtccgga cgagtctagg cgagcaggtc atcgtccccc
     1441 cctcagaatt ggagaggtgt cctggtgcgc cttcagtctg tgacattcag ctgaaccagg
     1501 tgtcgcctgc tgacttcact gtcctcagtg atgtgctgcc aatgttcagc gtggacttca
     1561 gcaagcaagt cagcagctcg gcagcgtgcc atagcaggca gtttgtacct ttggcgtctg
     1621 gccaagcaca ggtggttctg tcctggtggg acattgaaat ggaccctgag ggcaagatca
     1681 agtgcaccat ggcacccttt tgggcacaga cagatccgca ggagcttcag tggcgggacc
     1741 actggatgca gtgtgtgtac ttcctgccgc aggaggagcc tgttgtgcag ggctcacccc
     1801 ggtgcctggt agcccaccat gatgactact gtgtgtggta cagccttcag agaaccagcc
     1861 ctgatgagaa cgacagcgcc taccaagtgc gacctgtgtg tgactgtcag gctcacttgc
     1921 tctggaaccg gcctcggttt ggagaaatca atgatcagga cagaactgat cactatgccc
     1981 aggccctgag gactgtgctg ctgccaggta gcgtctgcct ttgtgtgagt gatggcagtc
     2041 tcctctccat gctggcccat cacctcggag cggagcaggt gtttacagtt gagagttcag
     2101 tagcttccta tagactgatg aaaaggatct tcaaggttaa ccacttggaa gataaaatca
     2161 gtgtcatcaa taaacggcct gagttgctga cagctgcaga cctggagggc aagaaggtct
     2221 ccctcctcct gggtgaaccc tttttcacca ccagcctgct gccatggcac aacctgtact
     2281 tctggtatgt ccgtacctct gtggaccagc acctagcacc tggagctgtg gtgatgcctc
     2341 aggctgcctc actgcatgcc gtgattgtgg agttcaggga cctgtggcgg atccggagtc
     2401 cttgcggtga ctgcgaaggt tttgatgtgc acatcatgga tgatatgatc aagcactccc
     2461 tggatttccg agagagcaga gaggcagagc cacacccact gtgggaatac ccctgcagaa
     2521 gcctctccaa gcctcaagag atcctgactt ttgatttcca gcagcccatc ccccaacagc
     2581 ctatgcaatc caagggcaca atggagctga caagacccgg gaagagccat ggggctgtcc
     2641 tgtggatgga gtatcagctc actccagaca gcacgatcag cactggcctc ataaaccctg
     2701 cagaagacaa gggggactgc tgctggaacc cccactgcaa gcaagctgtg tacttcctca
     2761 gcaccacgct ggatctcaga gtgcctctga atggccctcg gtcagtcagc tatgttgtgg
     2821 agtttcaccc cctcactgga gacatcacca tggagtttag gcttgcagac accttgagct
     2881 gaagatccgc ggccgcgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     2941 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     3001 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     3061 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     3121 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     3181 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     3241 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     3301 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     3361 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     3421 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata cctccggaat
     3481 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct
     3541 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat
     3601 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca
     3661 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt
     3721 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg
     3781 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt
     3841 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg
     3901 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt
     3961 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat
     4021 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt
     4081 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc
     4141 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga
     4201 cggcaccgcc ggacccggct ccaccggatc tcgctagagc tgaatctaag tcgacaatca
     4261 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4321 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4381 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4441 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4501 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4561 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4621 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     4681 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4741 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4801 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac
     4861 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4921 aagggctaat tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct
     4981 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5041 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5101 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     5161 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     5221 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5281 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5341 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     5401 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5461 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5521 agacttttgc agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     5581 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5641 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5701 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5761 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5821 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5881 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5941 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6001 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6061 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6121 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6181 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6241 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6301 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6361 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6421 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6481 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6541 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6601 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6661 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6721 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6781 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6841 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6901 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6961 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7021 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7081 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7141 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7201 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7261 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7321 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7381 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7441 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7501 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7561 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7621 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7681 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     7741 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     7801 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7861 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     7921 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7981 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     8041 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     8101 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     8161 cggccagtgc caagctgacg cgtgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     8221 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     8281 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     8341 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     8401 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8461 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8521 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8581 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     8641 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     8701 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     8761 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     8821 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8881 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8941 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     9001 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     9061 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccactgat cttcagacct
     9121 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     9181 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     9241 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     9301 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     9361 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     9421 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     9481 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     9541 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     9601 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     9661 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     9721 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     9781 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     9841 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggac
//
