LOCUS       sequence-180137-       12577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP 
            measurement using YcgR binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Christen M, Kulasekara HD, Christen B, Kulasekara BR, Hoffman LR, 
            Miller SI
  TITLE     Asymmetrical distribution of the second messenger c-di-GMP upon 
            bacterial cell division.
  JOURNAL   Science. 2010 Jun 4;328(5983):1295-7. doi: 10.1126/science.1188658.
  PUBMED    20522779
REFERENCE   2  (bases 1 to 12577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2010-06-4- 4"; volume: "328"; issue: 
            "5983"; pages: "1295-7"
COMMENT     Sequence Label: pMMB67EH-Spy
FEATURES             Location/Qualifiers
     source          1..12577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(18..23,24..39,40..46)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    54..70
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     59..81
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             join(114..116,117..119,120..830)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     CDS             join(1572..1574,1575..1577,1578..2288)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2345..2362)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2515..2601
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2693..2720
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2739..2830
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(3412..3945)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4134..4139,4140..4156,4157..4162))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     complement(4628..4647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5707..6101
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            6443..6530
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             7769..8740
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             9251..10090
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             10077..10928
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(11205..12287)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     12249..12268
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(12288..12365)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 tattctgaaa tgagctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
       61 cggataacaa tttcacacag gaaacagaat tcactttaag aaggagatat catatggtga
      121 gcaaaggcga agagctgttc accggcgtgg tgcccatcct ggtggagctg gacggcgacg
      181 tgaacggcca caagttcagc gtgagcggcg agggcgaggg cgacgccacc tacggcaagc
      241 tgaccctgaa gctgctgtgc accaccggca agctgcccgt gccctggccc accctggtga
      301 ccaccctggg ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
      361 acttcttcaa gagcgccatg cccgagggct acgtgcagga gcggaccatc ttcttcaagg
      421 acgacggcaa ctacaagacc cgggccgagg tgaagttcga gggcgacacc ctggtgaacc
      481 ggatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg
      541 agtacaacta caacagccac aacgtgtaca tcaccgccga caagcagaag aacggcatca
      601 aggccaactt caagatccgg cacaacatcg aggacggcgg cgtgcagctg gccgaccact
      661 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      721 gctaccagag caagctgttc aaggacccca acgagaagcg ggaccacatg gtgctgctgg
      781 agttcctgac cgccgccggc atcaccgagg gcatgaacga gctctataag actagttcgg
      841 gctacaacga gcagttcctg aagaagaacc cgctggccat cctgggcgtc ctgcgcgacc
      901 tgaacaagaa ccaggtcccg ctgcgcatct cgtgggccca cggccagttc atctcgaaga
      961 tcctggccgt cgacccggag aagctgatcg tcgactacgg cagccaggag tacgagaaca
     1021 gcgccgtgct gcgcgccggc caggtcgcca tcatcgccga gacccagggc gccaaggtcg
     1081 agttcaccct gccgcagctg gtcaccggcg agtaccagcg cctgccggcc ttcatcaccc
     1141 cgctgccgtc gtcgctgtgg ttcgtccagc gccgcgagta cttccgcatc ggcgccccgc
     1201 tgtatccgcc gtactacggc gtcaccaccc tgccggacac ccgcaccctg cgcttccgcc
     1261 tgttcgacct gtcgctgggc ggcatgggcg ccctgctgga gtcggccatc ccggacggcc
     1321 tgatcgaggg cgcccgcttc tcgcaggtcg agctgaacat gggccagtgg ggcatcttcc
     1381 acgtcgacgc ccagctgatc tcgatctcgg agcgcaaggt catcgacggc aagaacgaga
     1441 ccatcaccac cccgcgcctg tcgttccgct tcctgaacgt gtcgccggcc gtcgagcgcg
     1501 agctgcagcg catcatcttc tcgctggagc gcgaggcccg cgagcgcgcc aacaaggtcc
     1561 gcgagggtac catggtgagc aagggagagg aactgttcgg cggcatcgtg cccatcctgg
     1621 tggagctgga gggcgacgtg aacggccaca agttcagcgt gagcggcgag ggcgagggcg
     1681 acgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1741 cctggcccac cctggtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg
     1801 accacatgaa gcagcacgac ttcttcaaga gcgtgatgcc cgagggctac gtgcaggagc
     1861 ggaccatctt cttcaaggac gacggcaact acaagacccg ggccgaggtg aagttcgagg
     1921 gcgacaccct ggtgaaccgg atcgagctga agggcatcga cttcaaggag gacggcaaca
     1981 tcctgggcca caagctggag tacaactaca tcagccacaa cgtgtacatc accgccgaca
     2041 agcagaagaa cggcatcaag gccaacttca aggcccggca caacatcacc gacggcagcg
     2101 tgcagctggc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgatcctgc
     2161 ccgacaacca ctacctgagc acccagagca agctgagcaa ggaccccaac gagaagcggg
     2221 accacatggt gctgctggag ttcgtgaccg ccgccggcat cacccacggc atggacgaac
     2281 tgtacaaagg cggccgctaa ctcgagaagc ttggctgttt tggcggatga gagaagattt
     2341 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
     2401 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
     2461 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata
     2521 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2581 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     2641 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc
     2701 atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca
     2761 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2821 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2881 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2941 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3001 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt catgatgtct aacggccaag
     3061 gtaagcggcc cgcagaatgc gggtccgctt gaccgcagag ttagacccga aggccaaaag
     3121 ggagcgcccg gccttgaaga agggcgcagc ccaagaaaaa ccgctggacc acgaaagcgg
     3181 cgcagcgctg caagaaggat agctggtggc gcttgctgca caagcgctgg aggcccaaaa
     3241 ggctagaaag cctttggaac agggcggagc gctgaaataa gtggctgcgc gcataaagcg
     3301 ggtgatggga ctaacgcctg agttaagccg gagcgctttg cggccgcggc gttgtgacaa
     3361 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
     3421 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
     3481 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
     3541 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
     3601 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
     3661 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
     3721 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
     3781 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
     3841 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
     3901 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
     3961 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     4021 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
     4081 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     4141 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     4201 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     4261 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     4321 aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc
     4381 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
     4441 agcatcgttt gttcgcccag cttctgtatg gaacgggcat gcggatcagt gagggtttgc
     4501 aactgcgggt caaggatctg gatttcgatc acggcacgat catcgtgcgg gagggcaagg
     4561 gctccaagga tcgggccttg atgttacccg agagcttggc acccagcctg cgcgagcaga
     4621 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     4681 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4741 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4801 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4861 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4921 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4981 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5041 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5101 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5161 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5221 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5281 aagtttactc atatatactt tagattgatt tctgaaagcg accaggtgct cggcgtggca
     5341 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     5401 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     5461 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     5521 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     5581 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     5641 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccgtactaa
     5701 ctgtcacgaa cccctgcaat aactgtcacg cccccctgca ataactgtca cgaacccctg
     5761 caataactgt cacgccccca aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt
     5821 ggaaaaatcc atccatgatt atctaagaat aatccactag gcgcggttat cagcgccctt
     5881 gtggggcgct gctgcccttg cccaatatgc ccggccagag gccggatagc tggtctattc
     5941 gctgcgctag gctacacacc gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc
     6001 cgctaaaccc cacaccaaac cccgcagaaa tacgctggag cgcttttagc cgctttagcg
     6061 gcctttcccc ctacccgaag ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt
     6121 cgatcattca gcccggctca tccttctggc gtggcggcag accgaacaag gcgcggtcgt
     6181 ggtcgcgttc aaggtacgca tccattgccg ccatgagccg atcctccggc cactcgctgc
     6241 tgttcacctt ggccaaaatc atggccccca ccagcacctt gcgccttgtt tcgttcttgc
     6301 gctcttgctg ctgttccctt gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt
     6361 gttcttcgag cttggccagc cgatccgccg ccttgttgct ccccttaacc atcttgacac
     6421 cccattgtta atgtgctgtc tcgtaggcta tcatggaggc acagcggcgg caatcccgac
     6481 cctactttgt aggggagggc gcacttaccg gtttctcttc gagaaactgg cctaacggcc
     6541 acccttcggg cggtgcgctc tccgagggcc attgcatgga gccgaaaagc aaaagcaaca
     6601 gcgaggcagc atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca
     6661 atcggccagg gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga
     6721 tgaagtcttg cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta
     6781 ctgggatgct gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt
     6841 tgccctgccg gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca
     6901 gcacctgacc ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga
     6961 gaacccgcac tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc
     7021 cgctcagtgg ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac
     7081 cgaagcgctc aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc
     7141 caaccgggca ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc
     7201 gcagggcatc gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg
     7261 ccggggcatc cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca
     7321 gatcatcgac ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga
     7381 agaaatccag aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg
     7441 cgacactggc cgacgaagcc cggcaggtca tgagccagac ccagcaggcc agcgaggcgc
     7501 aggcggcgga gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca
     7561 aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt
     7621 cgcgggggtg gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg
     7681 tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg
     7741 gctcgatctg gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg
     7801 ccgacagctc aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac
     7861 cggccagatg atgaaccggg aatggtcagc cgccgaagtg ctccagaaca cgccatggct
     7921 caagcggatg aatgcccagg gcaatgacgt gtatatcagg cccgccgagc aggagcggca
     7981 tggtctggtg ctggtggacg acctcagcga gtttgacctg gatgacatga aagccgaggg
     8041 ccgggagcct gccctggtag tggaaaccag cccgaagaac tatcaggcat gggtcaaggt
     8101 ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc cggacgctgg ccagcgagta
     8161 cgacgccgac ccggccagcg ccgacagccg ccactatggc cgcttggcgg gcttcaccaa
     8221 ccgcaaggac aagcacacca cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc
     8281 caagggcaag accgccaccg ctggcccggc gctggtgcag caggctggcc agcagatcga
     8341 gcaggcccag cggcagcagg agaaggcccg caggctggcc agcctcgaac tgcccgagcg
     8401 gcagcttagc cgccaccggc gcacggcgct ggacgagtac cgcagcgaga tggccgggct
     8461 ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc agaagctggc
     8521 cagccggggc cgcagtgccg aggaaatcgg caaggccatg gccgaggcca gcccagcgct
     8581 ggcagagcgc aagcccggcc acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat
     8641 gggtctgccc agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg caccccgcca
     8701 gcgaggcatg gacaggggcg ggccagattt cagcatgtag tgcttgcgtt ggtactcacg
     8761 cctgttatac tatgagtact cacgcacaga agggggtttt atggaatacg aaaaaagcgc
     8821 ttcagggtcg gtctacctga tcaaaagtga caagggctat tggttgcccg gtggctttgg
     8881 ttatacgtca aacaaggccg aggctggccg cttttcagtc gctgatatgg ccagccttaa
     8941 ccttgacggc tgcaccttgt ccttgttccg cgaagacaag cctttcggcc ccggcaagtt
     9001 tctcggtgac tgatatgaaa gaccaaaagg acaagcagac cggcgacctg ctggccagcc
     9061 ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc
     9121 gcaagttctg gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg gaagaactca
     9181 gagcggcgca gggcgggggt agtgaccccg ccagcgccta accaccaact gcctgcaaag
     9241 gaggcaatca atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc
     9301 gccaccgctg gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc
     9361 gcccggtggt gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg
     9421 gccggatctg ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga
     9481 agacccgccc accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga
     9541 ggaacggcaa gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa
     9601 catcatggcc ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat
     9661 ggtgctggac acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc
     9721 ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct
     9781 gcaccatgcc agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg
     9841 gggcagctcg gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag
     9901 cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg
     9961 tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg
    10021 cggggtgctc aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg
    10081 tgaagcctaa gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg
    10141 cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga
    10201 cgtatgacta cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg
    10261 atgatctgcg catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg
    10321 gcccggaacc caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg
    10381 aggccgtcac cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa
    10441 tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc
    10501 tttggaaggt atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt
    10561 cggagtacgc cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg
    10621 cgcaggccgt catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc
    10681 tggacagcga aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca
    10741 aaaccggcaa ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg
    10801 gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc
    10861 tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg
    10921 caggctgacc ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc
    10981 ttattttcct gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa
    11041 tattttgaaa aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct
    11101 ttgcttccag atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc
    11161 ggccagcttg caattcgcgc taacttacat taattgcgtt gcgctcactg cccgctttcc
    11221 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    11281 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
    11341 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
    11401 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
    11461 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
    11521 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
    11581 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
    11641 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
    11701 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
    11761 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
    11821 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
    11881 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
    11941 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
    12001 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
    12061 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
    12121 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
    12181 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
    12241 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
    12301 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg
    12361 gtgtcaacgt aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc
    12421 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
    12481 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
    12541 atgttttttg cgccgacatc ataacggttc tggcaaa
//
