LOCUS       sequence-180138-       11119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP control expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Christen M, Kulasekara HD, Christen B, Kulasekara BR, Hoffman LR, 
            Miller SI
  TITLE     Asymmetrical distribution of the second messenger c-di-GMP upon 
            bacterial cell division.
  JOURNAL   Science. 2010 Jun 4;328(5983):1295-7. doi: 10.1126/science.1188658.
  PUBMED    20522779
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2010-06-4- 4"; volume: "328"; issue: 
            "5983"; pages: "1295-7"
COMMENT     Sequence Label: pMMB67EH-CFP
FEATURES             Location/Qualifiers
     source          1..11119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(29..34,35..50,51..57)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     70..92
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             join(125..127,128..130,131..841)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(431..450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(898..915)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1068..1154
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1246..1273
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1292..1383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(1965..2498)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2687..2692,2693..2709,2710..2715))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     complement(3181..3200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4260..4654
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            4996..5083
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6322..7293
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             7804..8643
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             8630..9481
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(9758..10840)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10802..10821
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10841..10918)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 gttctggcaa atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt
       61 ggaattgtga gcggataaca atttcacaca ggaaacagaa ttcactttaa gaaggagata
      121 tcatatggtg agcaagggag aggaactgtt cggcggcatc gtgcccatcc tggtggagct
      181 ggagggcgac gtgaacggcc acaagttcag cgtgagcggc gagggcgagg gcgacgccac
      241 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      301 caccctggtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
      361 gaagcagcac gacttcttca agagcgtgat gcccgagggc tacgtgcagg agcggaccat
      421 cttcttcaag gacgacggca actacaagac ccgggccgag gtgaagttcg agggcgacac
      481 cctggtgaac cggatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      541 ccacaagctg gagtacaact acatcagcca caacgtgtac atcaccgccg acaagcagaa
      601 gaacggcatc aaggccaact tcaaggcccg gcacaacatc accgacggca gcgtgcagct
      661 ggccgaccac taccagcaga acacccccat cggcgacggc cccgtgatcc tgcccgacaa
      721 ccactacctg agcacccaga gcaagctgag caaggacccc aacgagaagc gggaccacat
      781 ggtgctgctg gagttcgtga ccgccgccgg catcacccac ggcatggacg aactgtacaa
      841 aggcggccgc taactcgaga agcttggctg ttttggcgga tgagagaaga ttttcagcct
      901 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag
      961 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
     1021 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa
     1081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     1141 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     1201 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
     1261 cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat
     1321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1501 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1561 cccgaagaac gttttccaat gatgagcact tttcatgatg tctaacggcc aaggtaagcg
     1621 gcccgcagaa tgcgggtccg cttgaccgca gagttagacc cgaaggccaa aagggagcgc
     1681 ccggccttga agaagggcgc agcccaagaa aaaccgctgg accacgaaag cggcgcagcg
     1741 ctgcaagaag gatagctggt ggcgcttgct gcacaagcgc tggaggccca aaaggctaga
     1801 aagcctttgg aacagggcgg agcgctgaaa taagtggctg cgcgcataaa gcgggtgatg
     1861 ggactaacgc ctgagttaag ccggagcgct ttgcggccgc ggcgttgtga caatttaccg
     1921 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt
     1981 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
     2041 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
     2101 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
     2161 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa
     2221 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
     2281 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
     2341 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
     2401 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
     2461 ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa
     2521 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa
     2581 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     2641 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga
     2701 acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc
     2761 aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt
     2821 ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct
     2881 gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc
     2941 ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg
     3001 tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt tgcaactgcg
     3061 ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca agggctccaa
     3121 ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc agaaagttct
     3181 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     3241 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3301 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3361 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3421 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3481 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3541 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3601 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3661 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3721 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3781 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3841 ctcatatata ctttagattg atttctgaaa gcgaccaggt gctcggcgtg gcaagactcg
     3901 cagcgaaccc gtagaaagcc atgctccagc cgcccgcatt ggagaaattc ttcaaattcc
     3961 cgttgcacat agcccggcaa ttcctttccc tgctctgcca taagcgcagc gaatgccggg
     4021 taatactcgt caacgatctg atagagaagg gtttgctcgg gtcggtggct ctggtaacga
     4081 ccagtatccc gatcccggct ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt
     4141 gcaatactgt gtttacatac agtctatcgc ttagcggaaa gttcttttac cctcagccga
     4201 aatgcctgcc gttgctagac attgccagcc agtgcccgtc actcccgtac taactgtcac
     4261 gaacccctgc aataactgtc acgcccccct gcaataactg tcacgaaccc ctgcaataac
     4321 tgtcacgccc ccaaacctgc aaacccagca ggggcggggg ctggcggggt gttggaaaaa
     4381 tccatccatg attatctaag aataatccac taggcgcggt tatcagcgcc cttgtggggc
     4441 gctgctgccc ttgcccaata tgcccggcca gaggccggat agctggtcta ttcgctgcgc
     4501 taggctacac accgccccac cgctgcgcgg cagggggaaa ggcgggcaaa gcccgctaaa
     4561 ccccacacca aaccccgcag aaatacgctg gagcgctttt agccgcttta gcggcctttc
     4621 cccctacccg aagggtgggg gcgcgtgtgc agccccgcag ggcctgtctc ggtcgatcat
     4681 tcagcccggc tcatccttct ggcgtggcgg cagaccgaac aaggcgcggt cgtggtcgcg
     4741 ttcaaggtac gcatccattg ccgccatgag ccgatcctcc ggccactcgc tgctgttcac
     4801 cttggccaaa atcatggccc ccaccagcac cttgcgcctt gtttcgttct tgcgctcttg
     4861 ctgctgttcc cttgcccgca cccgctgaat ttcggcattg attcgcgctc gttgttcttc
     4921 gagcttggcc agccgatccg ccgccttgtt gctcccctta accatcttga caccccattg
     4981 ttaatgtgct gtctcgtagg ctatcatgga ggcacagcgg cggcaatccc gaccctactt
     5041 tgtaggggag ggcgcactta ccggtttctc ttcgagaaac tggcctaacg gccacccttc
     5101 gggcggtgcg ctctccgagg gccattgcat ggagccgaaa agcaaaagca acagcgaggc
     5161 agcatggcga tttatcacct tacggcgaaa accggcagca ggtcgggcgg ccaatcggcc
     5221 agggccaagg ccgactacat ccagcgcgaa ggcaagtatg cccgcgacat ggatgaagtc
     5281 ttgcacgccg aatccgggca catgccggag ttcgtcgagc ggcccgccga ctactgggat
     5341 gctgccgacc tgtatgaacg cgccaatggg cggctgttca aggaggtcga atttgccctg
     5401 ccggtcgagc tgaccctcga ccagcagaag gcgctggcgt ccgagttcgc ccagcacctg
     5461 accggtgccg agcgcctgcc gtatacgctg gccatccatg ccggtggcgg cgagaacccg
     5521 cactgccacc tgatgatctc cgagcggatc aatgacggca tcgagcggcc cgccgctcag
     5581 tggttcaagc ggtacaacgg caagaccccg gagaagggcg gggcacagaa gaccgaagcg
     5641 ctcaagccca aggcatggct tgagcagacc cgcgaggcat gggccgacca tgccaaccgg
     5701 gcattagagc gggctggcca cgacgcccgc attgaccaca gaacacttga ggcgcagggc
     5761 atcgagcgcc tgcccggtgt tcacctgggg ccgaacgtgg tggagatgga aggccggggc
     5821 atccgcaccg accgggcaga cgtggccctg aacatcgaca ccgccaacgc ccagatcatc
     5881 gacttacagg aataccggga ggcaatagac catgaacgca atcgacagag tgaagaaatc
     5941 cagaggcatc aacgagttag cggagcagat cgaaccgctg gcccagagca tggcgacact
     6001 ggccgacgaa gcccggcagg tcatgagcca gacccagcag gccagcgagg cgcaggcggc
     6061 ggagtggctg aaagcccagc gccagacagg ggcggcatgg gtggagctgg ccaaagagtt
     6121 gcgggaggta gccgccgagg tgagcagcgc cgcgcagagc gcccggagcg cgtcgcgggg
     6181 gtggcactgg aagctatggc taaccgtgat gctggcttcc atgatgccta cggtggtgct
     6241 gctgatcgca tcgttgctct tgctcgacct gacgccactg acaaccgagg acggctcgat
     6301 ctggctgcgc ttggtggccc gatgaagaac gacaggactt tgcaggccat aggccgacag
     6361 ctcaaggcca tgggctgtga gcgcttcgat atcggcgtca gggacgccac caccggccag
     6421 atgatgaacc gggaatggtc agccgccgaa gtgctccaga acacgccatg gctcaagcgg
     6481 atgaatgccc agggcaatga cgtgtatatc aggcccgccg agcaggagcg gcatggtctg
     6541 gtgctggtgg acgacctcag cgagtttgac ctggatgaca tgaaagccga gggccgggag
     6601 cctgccctgg tagtggaaac cagcccgaag aactatcagg catgggtcaa ggtggccgac
     6661 gccgcaggcg gtgaacttcg ggggcagatt gcccggacgc tggccagcga gtacgacgcc
     6721 gacccggcca gcgccgacag ccgccactat ggccgcttgg cgggcttcac caaccgcaag
     6781 gacaagcaca ccacccgcgc cggttatcag ccgtgggtgc tgctgcgtga atccaagggc
     6841 aagaccgcca ccgctggccc ggcgctggtg cagcaggctg gccagcagat cgagcaggcc
     6901 cagcggcagc aggagaaggc ccgcaggctg gccagcctcg aactgcccga gcggcagctt
     6961 agccgccacc ggcgcacggc gctggacgag taccgcagcg agatggccgg gctggtcaag
     7021 cgcttcggtg atgacctcag caagtgcgac tttatcgccg cgcagaagct ggccagccgg
     7081 ggccgcagtg ccgaggaaat cggcaaggcc atggccgagg ccagcccagc gctggcagag
     7141 cgcaagcccg gccacgaagc ggattacatc gagcgcaccg tcagcaaggt catgggtctg
     7201 cccagcgtcc agcttgcgcg ggccgagctg gcacgggcac cggcaccccg ccagcgaggc
     7261 atggacaggg gcgggccaga tttcagcatg tagtgcttgc gttggtactc acgcctgtta
     7321 tactatgagt actcacgcac agaagggggt tttatggaat acgaaaaaag cgcttcaggg
     7381 tcggtctacc tgatcaaaag tgacaagggc tattggttgc ccggtggctt tggttatacg
     7441 tcaaacaagg ccgaggctgg ccgcttttca gtcgctgata tggccagcct taaccttgac
     7501 ggctgcacct tgtccttgtt ccgcgaagac aagcctttcg gccccggcaa gtttctcggt
     7561 gactgatatg aaagaccaaa aggacaagca gaccggcgac ctgctggcca gccctgacgc
     7621 tgtacgccaa gcgcgatatg ccgagcgcat gaaggccaaa gggatgcgtc agcgcaagtt
     7681 ctggctgacc gacgacgaat acgaggcgct gcgcgagtgc ctggaagaac tcagagcggc
     7741 gcagggcggg ggtagtgacc ccgccagcgc ctaaccacca actgcctgca aaggaggcaa
     7801 tcaatggcta cccataagcc tatcaatatt ctggaggcgt tcgcagcagc gccgccaccg
     7861 ctggactacg ttttgcccaa catggtggcc ggtacggtcg gggcgctggt gtcgcccggt
     7921 ggtgccggta aatccatgct ggccctgcaa ctggccgcac agattgcagg cgggccggat
     7981 ctgctggagg tgggcgaact gcccaccggc ccggtgatct acctgcccgc cgaagacccg
     8041 cccaccgcca ttcatcaccg cctgcacgcc cttggggcgc acctcagcgc cgaggaacgg
     8101 caagccgtgg ctgacggcct gctgatccag ccgctgatcg gcagcctgcc caacatcatg
     8161 gccccggagt ggttcgacgg cctcaagcgc gccgccgagg gccgccgcct gatggtgctg
     8221 gacacgctgc gccggttcca catcgaggaa gaaaacgcca gcggccccat ggcccaggtc
     8281 atcggtcgca tggaggccat cgccgccgat accgggtgct ctatcgtgtt cctgcaccat
     8341 gccagcaagg gcgcggccat gatgggcgca ggcgaccagc agcaggccag ccggggcagc
     8401 tcggtactgg tcgataacat ccgctggcag tcctacctgt cgagcatgac cagcgccgag
     8461 gccgaggaat ggggtgtgga cgacgaccag cgccggttct tcgtccgctt cggtgtgagc
     8521 aaggccaact atggcgcacc gttcgctgat cggtggttca ggcggcatga cggcggggtg
     8581 ctcaagcccg ccgtgctgga gaggcagcgc aagagcaagg gggtgccccg tggtgaagcc
     8641 taagaacaag cacagcctca gccacgtccg gcacgacccg gcgcactgtc tggcccccgg
     8701 cctgttccgt gccctcaagc ggggcgagcg caagcgcagc aagctggacg tgacgtatga
     8761 ctacggcgac ggcaagcgga tcgagttcag cggcccggag ccgctgggcg ctgatgatct
     8821 gcgcatcctg caagggctgg tggccatggc tgggcctaat ggcctagtgc ttggcccgga
     8881 acccaagacc gaaggcggac ggcagctccg gctgttcctg gaacccaagt gggaggccgt
     8941 caccgctgat gccatggtgg tcaaaggtag ctatcgggcg ctggcaaagg aaatcggggc
     9001 agaggtcgat agtggtgggg cgctcaagca catacaggac tgcatcgagc gcctttggaa
     9061 ggtatccatc atcgcccaga atggccgcaa gcggcagggg tttcggctgc tgtcggagta
     9121 cgccagcgac gaggcggacg ggcgcctgta cgtggccctg aaccccttga tcgcgcaggc
     9181 cgtcatgggt ggcggccagc atgtgcgcat cagcatggac gaggtgcggg cgctggacag
     9241 cgaaaccgcc cgcctgctgc accagcggct gtgtggctgg atcgaccccg gcaaaaccgg
     9301 caaggcttcc atagatacct tgtgcggcta tgtctggccg tcagaggcca gtggttcgac
     9361 catgcgcaag cgccgccagc gggtgcgcga ggcgttgccg gagctggtcg cgctgggctg
     9421 gacggtaacc gagttcgcgg cgggcaagta cgacatcacc cggcccaagg cggcaggctg
     9481 acccccccca ctctattgta aacaagacat ttttatcttt tatattcaat ggcttatttt
     9541 cctgctaatt ggtaatacca tgaaaaatac catgctcaga aaaggcttaa caatattttg
     9601 aaaaattgcc tactgagcgc tgccgcacag ctccataggc cgctttcctg gctttgcttc
     9661 cagatgtatg ctcttctgct cccgaacgcc agcaagacgt agcccagcgc gtcggccagc
     9721 ttgcaattcg cgctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9781 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9841 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     9901 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     9961 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
    10021 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
    10081 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
    10141 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
    10201 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
    10261 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
    10321 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
    10381 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
    10441 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
    10501 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
    10561 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
    10621 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
    10681 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
    10741 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
    10801 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
    10861 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcaccattcg atggtgtcaa
    10921 cgtaaatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg atccgggctt atcgactgca
    10981 cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg
    11041 tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt
    11101 ttgcgccgac atcataacg
//
