LOCUS       sequence-180139-        6780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human mutant of E2F1 lacking amino acids 284 to 359.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M
  TITLE     Nuclear localization of gamma-tubulin affects E2F transcriptional 
            activity and S-phase progression.
  JOURNAL   FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 
            2011 Jul 25.
  PUBMED    21788450
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.11-187484"; journalName: "FASEB J"; date: "2011-11"; 
            volume: "25"; issue: "11"; pages: "3815-27"
COMMENT     Sequence Label: pcDNA3.1 E2F1 Flag(Delta 284−359)(HindIII- BamHI- 
            ...
FEATURES             Location/Qualifiers
     source          1..6780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(317..336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(996..1787,1788..1856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1619..1638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1857..1961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2036..2055)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2227..2606
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2607..2810
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2761..2781
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2855..2874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2855..2873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3976..3999
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4063..4081)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4064..4081)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4101..4118)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4107..4331
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4377..4805
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4464..4483)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4674..4695
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4814..4834)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4819..5148
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4999..5134
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5061..5080
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5215..6009
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5269..5288)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5879..5898
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6183..6304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6220..6239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6274..6293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6353..6369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6353..6369)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6366..6388)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6377..6393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6401..6407,6408..6425,6426..6431))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6446..6467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     2041 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     2101 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     2161 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     2221 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     2281 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2341 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2401 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2461 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2521 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2581 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2641 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2701 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2761 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
     2821 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
     2881 aagcttacca tggccttggc cggggcccct gcgggcggcc catgcgcgcc ggcgctggag
     2941 gccctgctcg gggccggcgc gctgcggctg ctcgactcct cgcagatcgt catcatctcc
     3001 gccgcgcagg acgccagcgc cccgccggct cccaccggcc ccgcggcgcc cgccgccggc
     3061 ccctgcgacc ctgacctgct gctcttcgcc acaccgcagg cgccccggcc cacacccagt
     3121 gcgccgcggc ccgcgctcgg ccgcccgccg gtgaagcgga ggctggacct ggaaactgac
     3181 catcagtacc tggccgagag cagtgggcca gctcggggca gaggccgcca tccaggaaaa
     3241 ggtgtgaaat ccccggggga gaagtcacgc tatgagacct cactgaatct gaccaccaag
     3301 cgcttcctgg agctgctgag ccactcggct gacggtgtcg tcgacctgaa ctgggctgcc
     3361 gaggtgctga aggtgcagaa gcggcgcatc tatgacatca ccaacgtcct tgagggcatc
     3421 cagctcattg ccaagaagtc caagaaccac atccagtggc tgggcagcca caccacagtg
     3481 ggcgtcggcg gacggcttga ggggttgacc caggacctcc gacagctgca ggagagcgag
     3541 cagcagctgg accacctgat gaatatctgt actacgcagc tgcgcctgct ctccgaggac
     3601 actgacagcc agcgcctggc ctacgtgacg tgtcaggacc ttcgtagcat tgcagaccct
     3661 gcagagcaga tggttatggt gatcaaagcc cctcctgaga cccagctcca agccgtggac
     3721 tcttcggaga actttcaggg atcccggatg ggcagcctgc gggctcccgt ggacgaggac
     3781 cgcctgtccc cgctggtggc ggccgactcg ctcctggagc atgtgcggga ggacttctcc
     3841 ggcctcctcc ctgaggagtt catcagcctt tccccacccc acgaggccct cgactaccac
     3901 ttcggcctcg aggagggcga gggcatcaga gacctcttcg actgtgactt tggggacctc
     3961 acccccctgg atttcgatta taaagatgat gatgataaat gagaattctg cagatatcca
     4021 tcacactggc ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa
     4081 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4141 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4201 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4261 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4321 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4381 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4441 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4501 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4561 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4621 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4681 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4741 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4801 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4861 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4921 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4981 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5041 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5101 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5161 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     5221 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     5281 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5341 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     5401 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5461 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5521 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5581 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5641 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5701 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     5761 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5821 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5881 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5941 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6001 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6061 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6121 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6181 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6241 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6301 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6361 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6421 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6481 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6541 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6601 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6661 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6721 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
//
