LOCUS       sequence-180161-        3306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses shRNA against TOM40.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Yano H, Baranov SV, Baranova OV, Kim J, Pan Y, Yablonska S, Carlisle
            DL, Ferrante RJ, Kim AH, Friedlander RM
  TITLE     Inhibition of mitochondrial protein import by mutant huntingtin.
  JOURNAL   Nat Neurosci. 2014 Jun;17(6):822-31. doi: 10.1038/nn.3721. Epub 2014
            May 18.
  PUBMED    24836077
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3721"; 
            journalName: "Nat Neurosci"; date: "2014-06"; volume: "17"; issue: 
            "6"; pages: "822-31"
COMMENT     Sequence Label: pBSU6-shTOM40.2
FEATURES             Location/Qualifiers
     source          1..3306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      452..1040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     941..960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1194..1211
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1328..1349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1364..1369,1370..1387,1388..1394)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1402..1418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1407..1429
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1426..1442
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1426..1442
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1461..1481
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1463..1481
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1518..1534
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1611..1924)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(1672..1692)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(1921..1937)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1954..1973)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1955..1973)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1983..2000)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1983..1999)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1992..2014)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2141..2596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2228..2247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2438..2459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2622..2726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2727..2795,2796..3306,1..281)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2945..2964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
       61 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      121 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      181 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
      241 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
      301 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
      361 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      421 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      481 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      541 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      601 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      661 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      721 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      781 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      841 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      901 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      961 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1021 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1081 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1141 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1201 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1261 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     1321 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     1381 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     1441 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc
     1501 accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa ttcaaaaagc
     1561 tgagtcccac agaggcgtta agcttgaata cgcctctgtg ggactcagca aacaaggctt
     1621 ttctccaagg gatatttata gtctcaaaac acacaattac tttacagtta gggtgagttt
     1681 ccttttgtgc tgttttttaa aataataatt tagtatttgt atctcttata gaaatccaag
     1741 cctatcatgt aaaatgtagc tagtattaaa aagaacagat tatctgtctt ttatcgcaca
     1801 ttaagcctct atagttacta ggaaatatta tatgcaaatt aaccggggca ggggagtagc
     1861 cgagcttctc ccacaagtct gtgcgagggg gccggcgcgg gcctagagat ggcggcgtcg
     1921 gatccactag ttctagagcg ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     1981 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2041 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2101 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     2161 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2221 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2281 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2341 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2401 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2461 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2521 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2581 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2641 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2701 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2761 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2821 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2881 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2941 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3001 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3061 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3121 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3181 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3241 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3301 taactg
//
