LOCUS       sequence-180163-        3306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses shRNA against TOM40..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Yano H, Baranov SV, Baranova OV, Kim J, Pan Y, Yablonska S, Carlisle
            DL, Ferrante RJ, Kim AH, Friedlander RM
  TITLE     Inhibition of mitochondrial protein import by mutant huntingtin.
  JOURNAL   Nat Neurosci. 2014 Jun;17(6):822-31. doi: 10.1038/nn.3721. Epub 2014
            May 18.
  PUBMED    24836077
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3721"; 
            journalName: "Nat Neurosci"; date: "2014-06"; volume: "17"; issue: 
            "6"; pages: "822-31"
COMMENT     Sequence Label: pBSU6-shTOM40.3
FEATURES             Location/Qualifiers
     source          1..3306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    519..540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(555..560,561..578,579..585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     598..620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     617..633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     617..633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     652..672
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        654..672
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     709..725
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(802..1115)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(863..883)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(1112..1128)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1145..1164)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1146..1164)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1174..1191)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1174..1190)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1183..1205)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1332..1787
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1419..1438)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1629..1650
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1813..1917
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1918..1986,1987..2778)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2136..2155)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2949..3306,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      661 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt
      721 ggatcccccg ggctgcagga attcaaaaag gcactgtcat gtctctagct aagcttgaat
      781 gctagagaca tgacagtgcc aaacaaggct tttctccaag ggatatttat agtctcaaaa
      841 cacacaatta ctttacagtt agggtgagtt tccttttgtg ctgtttttta aaataataat
      901 ttagtatttg tatctcttat agaaatccaa gcctatcatg taaaatgtag ctagtattaa
      961 aaagaacaga ttatctgtct tttatcgcac attaagcctc tatagttact aggaaatatt
     1021 atatgcaaat taaccggggc aggggagtag ccgagcttct cccacaagtc tgtgcgaggg
     1081 ggccggcgcg ggcctagaga tggcggcgtc ggatccacta gttctagagc gggtacccaa
     1141 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     1201 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1261 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1321 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1381 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1441 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1501 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1561 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1621 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1681 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     1741 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     1801 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1861 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1921 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1981 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2041 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2101 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2161 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2221 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2281 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2341 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2401 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2461 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2521 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2581 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2641 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2701 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2761 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2821 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2881 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2941 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3001 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3061 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3121 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3181 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3301 cgaacg
//
