LOCUS       sequence-180164-        4974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express shRNA Scrambled control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Yano H, Baranov SV, Baranova OV, Kim J, Pan Y, Yablonska S, Carlisle
            DL, Ferrante RJ, Kim AH, Friedlander RM
  TITLE     Inhibition of mitochondrial protein import by mutant huntingtin.
  JOURNAL   Nat Neurosci. 2014 Jun;17(6):822-31. doi: 10.1038/nn.3721. Epub 2014
            May 18.
  PUBMED    24836077
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3721"; 
            journalName: "Nat Neurosci"; date: "2014-06"; volume: "17"; issue: 
            "6"; pages: "822-31"
COMMENT     Sequence Label: pBSU6-shScr/CMV-eGFP
FEATURES             Location/Qualifiers
     source          1..4974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(156..175)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     503..524
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    692..773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(729..748)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(806..822)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(859..879)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(859..877)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(898..914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(898..914)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(911..933)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    922..938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(946..952,953..970,971..976))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    991..1012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1129..1146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1300..1888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1380..1399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2059..2850,2851..2919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2682..2701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2920..3024)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3050..3505)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3187..3208)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3399..3418
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3632..3654
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3646..3663
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3647..3663
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3673..3692
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3673..3691
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3709..3725
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3722..4035
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     3954..3974
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     4047..4063
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4048..4064
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4206..4509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4510..4713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4664..4684
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    4736..4816
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      4818..4827
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4824..4826,4827..4829,4830..4974,1..569)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4824..4826,4827..4829,4830..4974,1..566)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4869..4890)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
       61 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      121 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      181 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      241 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      301 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      361 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      421 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      481 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      541 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
      601 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
      661 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
      721 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      781 ttgtggtttg tccaaacttc cgggggatcc actagttcta gagcggccgc caccgcggtg
      841 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
      901 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      961 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1021 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1081 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1141 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1201 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1261 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1441 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1501 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1561 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1621 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1681 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1741 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1801 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1861 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1921 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1981 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2041 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2101 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2161 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2221 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2281 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2341 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2401 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2461 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2521 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2581 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2641 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2701 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2761 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2821 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2881 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2941 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3001 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     3061 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3121 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3181 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3241 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3301 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3361 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3421 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3481 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     3541 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     3601 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     3661 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtacccgctc tagaactagt
     3721 ggatccgacg ccgccatctc taggcccgcg ccggccccct cgcacagact tgtgggagaa
     3781 gctcggctac tcccctgccc cggttaattt gcatataata tttcctagta actatagagg
     3841 cttaatgtgc gataaaagac agataatctg ttctttttaa tactagctac attttacatg
     3901 ataggcttgg atttctataa gagatacaaa tactaaatta ttattttaaa aaacagcaca
     3961 aaaggaaact caccctaact gtaaagtaat tgtgtgtttt gagactataa atatcccttg
     4021 gagaaaagcc ttgtttgggc cccccctcga ggtcgacggt atcgattaag attggcggaa
     4081 tagttcaaga gactattccg ccaatcttaa tttttttgga aatgcagggt attaccgcca
     4141 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     4201 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     4261 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     4321 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     4381 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     4441 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     4501 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     4561 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     4621 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     4681 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
     4741 cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg
     4801 cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     4861 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     4921 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc acca
//
