LOCUS       sequence-180180-        6818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP4.1.1
FEATURES             Location/Qualifiers
     source          1..6818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1834..1851)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1840..2064
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2196..2784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2276..2295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3113..3160
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3185..3204
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3392..4180)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3503..3522)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4107..4126
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4215..4572)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4229..4364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4381..4572
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4529..4549
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4632..5423,5424..5492))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5255..5274
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5493..5597)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5624..6079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5711..5730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5921..5942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6091..6470
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6471..6674
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6625..6645
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6719..6738
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6719..6737
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acaggggccg ccatgggttg ctctttttct atcttcctct tggcacttct ctcgtgcctg
       61 actgtccccg cttcggccat taactatcgc aatgtctcgg gcatctacca tgtcaccaac
      121 gattgcccga attcaagcat agtgtatgag accgaacatc acatcttgca cattccaggt
      181 tgcgtgccct gtgtgagagt ggggaatcag tcaagctgct gggtggccct tactcctacc
      241 gtcgctacgc catacatcgg cgcgccgctt gagtccttgc ggagtcatgt ggatctgatg
      301 gtgggagctg ccactgcctg ctcagccctt tacatcgggg atttgtgtgg cggtgtgttt
      361 ttggttggtc agatgttctc cttccgaccg cggcgccact ggactaccca ggattgcaat
      421 tgttccatct atacagggca catcactggc cacagaatgg cctgggacat gatgatgaac
      481 tggagtccaa cagccacctt agttctcgcc cagcttatga ggatcccagg tgccttagta
      541 gacctacttg ctggaggtca ctggggtgtc ctcgtgggag tggcctattt ctgcatgcaa
      601 gccaattggg ccaaagtcat cttagtccta ttcctctttg caggggttga tgctcagaca
      661 cgtttgactg ggggcacacc tgcccacggc gcacagagca tagctaaatt gtttagcgct
      721 ggacctcggc aaaatgtaca gctcattaac agcaacggga gctggcatat aaacaggact
      781 gccctcaact gcaatgacag cttaaacact gggttcttga ctggcttgtt ctacgtctcc
      841 aggttcaaca gctcagggtg tcccgagcgg ctcgcaagtt gcaagagcct tgacagcttt
      901 aaccagggct ggggcccact aggggccgct aacatcagcg gcccgtctga cgacaagcct
      961 tattgctggc actacgcgcc tcgaccgtgc gggattgtgc cggcatccag tgtgtgtggc
     1021 cccgtgtatt gtttcactcc cagccctgtc gtggtcggaa ccacagaccg tttcggggtc
     1081 ccgacttaca cctgggggga taatgagtct gatgtcttcc ttttgaacgc gaccagaccg
     1141 ccacgtggtg cgtggttcgg gtgcgtatgg atgaacagta ccgggtttac aaaaacctgt
     1201 ggtgctcccc catgtgaggt caagaccaac aatggaacct ggagctgccc caccgattgc
     1261 ttcaggaagc acccagcgac cacgtacacc aagtgcgggt cggggccctg gatcacacca
     1321 cgatgcttgg tccactatcc gtaccggctg tggcatttcc catgtaccct caattttacc
     1381 atcttcaagg tcaggacata catcggcggc atagagcacc ggatggaagt ggcatgcaac
     1441 tggactaggg gggaagtctg tggcttagag catagggatc gtgcagaact gtcacctctg
     1501 ctcctttcca ctacgacgtg gcaagtcctc ccctgctctt ttaccactct acctgccctt
     1561 tccaccggct tgatccacct ccaccaaaat atcgtggacg tccaatacct ctacggtgtt
     1621 ggatctgcgg tagtatcttg ggctctcaag tgggagtatg tggtgcttgt gtttctgctt
     1681 cttgcagacg cgaggatctc tgcttgcctg tggatgatgt tcatggtaag tcaagttgag
     1741 gcgtaatcta gaaaccttaa gccgaattct gcagatatcc agcacagtgg cggccgctcg
     1801 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     1861 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     1921 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     1981 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2041 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagtggc ggtaatacgg
     2101 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2161 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2221 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2281 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2341 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2401 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2461 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2521 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2581 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     2641 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2701 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2761 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2821 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2881 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2941 cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc
     3001 gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt
     3061 ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac
     3121 gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc
     3181 ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa gaactccagc
     3241 atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc
     3301 aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct
     3361 tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     3421 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     3481 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     3541 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     3601 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg
     3661 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttga tcatcctgat
     3721 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     3781 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     3841 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     3901 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     3961 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg
     4021 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     4081 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     4141 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     4201 tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     4261 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     4321 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     4381 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     4441 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
     4501 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctggttc
     4561 tttccgcctc aggactcttc ctttttcaat aaatcaatct aaagtatata tgagtaaact
     4621 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4681 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4741 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4801 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4861 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4921 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4981 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5041 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5101 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5161 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5221 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5281 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5341 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5401 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5461 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5521 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5581 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     5641 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5701 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5761 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5821 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5881 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5941 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6001 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6061 atattaacgc ttacaattta cgcgcgcgtt gacattgatt attgactagt tattaatagt
     6121 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     6181 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     6241 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt
     6301 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     6361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     6421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     6481 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     6541 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     6601 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     6661 atataagcag agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta
     6721 atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc ttggtaccga
     6781 gctcggatcc actagtccag tgtggtggaa ttcggctt
//
