LOCUS       sequence-180185-        6821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP4.2.2
FEATURES             Location/Qualifiers
     source          1..6821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1837..1854)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1843..2067
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2199..2787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2279..2298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3116..3163
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3188..3207
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3395..4183)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3506..3525)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4110..4129
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4218..4575)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4232..4367
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4384..4575
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4532..4552
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4635..5426,5427..5495))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5258..5277
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5496..5600)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5627..6082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5714..5733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5924..5945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6094..6473
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6474..6677
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6628..6648
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6722..6741
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6722..6740
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acaggggccg ccatgggttg ctctttttct atcttccttt tggcacttct ctcgtgcctg
       61 actgtccccg cgtcggccac taactatcgc aatgtctcgg gcatctacca cgtcaccaat
      121 gactgcccga actcaagtat agtgtatgag gccgaccatc acatcttgca cctcccaggc
      181 tgcgtgccct gcgtgaggac ggggaatcag tcacactgct gggtggctct tactcctact
      241 gtcgcagcgc catacatcgg cgcaccgctt gagtctcttc ggagtcatgt ggatttgatg
      301 gtgggaactg ccactgcttg ctcggccctt tacatcgggg atctgtgtgg tggcttgttc
      361 ctggttggtc agatgttctc tttccgaccg cgacgccact ggactaccca ggagtgcaat
      421 tgttccatct atacagggca cctcactggc cacagaatgg cttgggacat gatgatgaac
      481 tggagtccaa caaccacttt agttcttgcc caggtcatga ggatcccagg tactctggta
      541 gacctgctcg ctggaggtca ctggggcgtc ctcgtgggag tagcctattt cagcatgcaa
      601 gccaattggg ccaaggtcat cttagtccta ttcctctttg caggagttga cgccagcact
      661 cacatatctg gagggacagc tggccgagct accgctggct tcactgagct ctttacacca
      721 ggagctaagc aaaatgtgca gctcattaac agcaatggga gttggcatat aaacaggact
      781 gccctcaact gcaatgatag tttaaacact ggattcctgg ctggcctgtt ctaccactac
      841 agctttaata gctcagggtg ccccgaacgg ctcgcgagct gcaagagcct tgacgaccat
      901 caccaaggct ggggcccact cgggatcgct aacatcagcg gcccgtctaa cgacaggcct
      961 tattgctggc actacgcgcc tcggccgtgc gggattgtgc cggcatccag tgtgtgtggc
     1021 cccgtctact gtttcactcc cagccccgtc gtggtcggaa ccactgaccg cctcggggtc
     1081 cccacttaca actgggggga gaatgagact gatgtcttct tactcaactc aaccagaccg
     1141 ccgcgggggg catggtttgg atgcgtgtgg atgaatggca ccgggtttac aaagacttgt
     1201 ggcgcccctc catgcgtggt caacgccaat accaataaca cttggcactg ccccaccgat
     1261 tgcttcagga agcatccggg gaccacttac gccaagtgcg ggtcagggcc gtggatcaca
     1321 ccacggtgct tgattgacta cccgtaccgg ctgtggcatt tcccatgtac catcaatttt
     1381 accatcttta aggtcaggac atttgtcggc gggatagagc atcggatggc agcggcatgc
     1441 aactggacca ggggggaagt ctgtggcttg gagcataggg atcgtgcaga gttatcacct
     1501 ctgctccttt ccaccaccac gtggcaaatc ctcccctgct ctttcaccac tctacctgcc
     1561 ctttccaccg gcttgatcca cctccaccaa aatatcgtgg acgtccagta cctctacggt
     1621 gttggatctg cggtggtatc ttgggccctc aaatgggaat atgtggtgct cgtgttcctg
     1681 cttctcgcgg acgcgagagt ctccgcttgc ctatggatga tgtttatggt aagtcaagtt
     1741 gaggcgtaat ctagaaacct taagccgaat tctgcagata tccagcacag tggcggccgc
     1801 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     1861 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1921 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1981 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     2041 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata
     2101 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2401 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2461 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2521 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2581 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2641 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2701 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2761 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2821 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2881 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2941 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
     3001 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
     3061 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
     3121 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct
     3181 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc
     3241 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     3301 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     3361 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     3421 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     3481 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     3541 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     3601 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     3661 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tgatcatcct
     3721 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     3781 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     3841 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     3901 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     3961 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac
     4021 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     4081 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     4141 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     4201 ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     4261 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     4321 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     4381 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     4441 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
     4501 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg
     4561 ttctttccgc ctcaggactc ttcctttttc aataaatcaa tctaaagtat atatgagtaa
     4621 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4681 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4741 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4801 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4861 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4921 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4981 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5041 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5101 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5161 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5221 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5281 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5341 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5401 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5461 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5521 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5581 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     5641 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5701 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5761 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5821 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5881 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5941 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6001 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6061 aaaatattaa cgcttacaat ttacgcgcgc gttgacattg attattgact agttattaat
     6121 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     6181 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     6241 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggact
     6301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     6361 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     6421 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     6481 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     6541 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     6601 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     6661 tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa
     6721 ttaatacgac tcactatagg gagacccaag ctggctagcg tttaaactta agcttggtac
     6781 cgagctcgga tccactagtc cagtgtggtg gaattcggct t
//
