LOCUS       sequence-180202-       11626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP in eyes and ubiquitous expression of P-transposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Stern DL, Crocker J, Ding Y, Frankel N, Kappes G, Kim E, Kuzmickas 
            R, Lemire A, Mast JD, Picard S
  TITLE     Genetic and Transgenic Reagents for Drosophila simulans, D. 
            mauritiana, D. yakuba, D. santomea, and D. virilis.
  JOURNAL   G3 (Bethesda). 2017 Apr 3;7(4):1339-1347. doi: 
            10.1534/g3.116.038885.
  PUBMED    28280212
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2017-04-3- 3"; volume: "7"; 
            issue: "4"; pages: "1339-1347"
COMMENT     Sequence Label: pBac{3XP3::EGFP, Pactin::Ptrsps}
FEATURES             Location/Qualifiers
     source          1..11626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    843..964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(880..899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     934..953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        1048..3557
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     repeat_region   6767..6801
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6982..6998)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6982..6998)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6995..7017)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7006..7022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7030..7036,7037..7054,7055..7060))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7075..7096
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7213..7230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7384..7972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7464..7483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8143..8934,8935..9003))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8766..8785
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9004..9108)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9176..9194
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9232..9254)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9354..9373
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9567..9589
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9581..9598
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9582..9598
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   10302..10364
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      join(11621..11626,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      781 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      841 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      901 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      961 cttaaagctt atcgatacgc gtacggcgcg cctaggccgg ccgaattcta tattctaaaa
     1021 acacaaatga tacttctaaa aaaaaatcat gaatggcatc aactctgaat caaatctttg
     1081 cagatgcacc tacttctcat ttccactgtc acatcatttt tccagatctc gctgcctgtt
     1141 atgtggccca caaaccaaga cacgttttat ggccattaaa gctggctgat cgtcgccaaa
     1201 caccaaatac ataatgaata tgtacacatt cgagaaagaa gcgatcaaag aagcgtcttc
     1261 gggcggagta ggagaatgcg gaggagaagg agaacgagct gatctagtat ctctccacaa
     1321 tccaatgcca actgaccaac tggccatatt cggagcaatt tgaagccaat ttccatcgcc
     1381 tggcgatcgc tccattcttg gctatatgtt tttcaccgtt acccggggcc attttcaaag
     1441 actcgtcggc aagataagat tgtgtcactc gctgtctctc ttcatttgtc gaagaatgct
     1501 gaggaatttc gcgatgacgt cggcgagtat tttgaagaat gagaataatt tgtatttata
     1561 cgaaaatcag ttagtggaat tttctacaaa aacatgttat ctatagataa ttttgttgca
     1621 aaatatgttg actatgacaa agattgtatg tatatacctt taatgtattc tcattttctt
     1681 atgtatttat aatggcaatg atgatactga tgatatttta agatgatgcc agaccaaaag
     1741 gcttgaattt ctgcgtcttt tgccgaacgc agtgcatgtg caattgttgt tttttggaat
     1801 attcaatttt cggactgtcc gctttgattt cagtttcttg gcttattcaa aaagcaaagt
     1861 aaagccaaaa aagcgagatg gcaataccaa atgcggcaaa acggtagtgg aaggaaaggg
     1921 gtgcggggca gcggaaggaa gggtggggcg gggcgtggcg gggtctgtgg ctgggcgcga
     1981 cgtcaccgac gttggagcca ctcctttgac catgtgtgcg tgtgtgtatt attcgtgtct
     2041 cgccactcgc cggttgtttt tttcttttta tgctgcgctc tctctagcgc catctcgctt
     2101 acgcatgctc aacgcaccgc atgttgccgt ttccttttat gcgtcatttt ggctcgaaat
     2161 aggcaattat ttaaacaaag attagtcaac gaaaacgcta aaataaataa gtctacaata
     2221 tggttactta ttgccatgtg tgtgcagcca acgatagcaa caaaagcaac aacacaggtg
     2281 gctttccctc tttcactttt tgtttgcaag ccgcgtgcga gcaagacggc acgaccggca
     2341 aacgcaatta cgctgacaaa gagcagacga agttttggcg aaaaacatca aggcgcctga
     2401 tacgaatgca tttgcaataa caattgcgat atttaatatt gtttatgaag ctgtttgact
     2461 tcaaaacaca caaaaaaaaa aataaaacaa attatttgaa agagaattag gaatcggacg
     2521 cttatcgtta gggtaacaac aagaaatgct tactgagtca cagcctctgg aaaactgccg
     2581 caagccagag agagagagaa aaagagggag agcagcttag accgcatgtg cttgtgtgtg
     2641 aggcgtctct ctcttcgtct ctgttgcgca aacgcataga ctgcactgaa aaaatcgatt
     2701 acctattttt tatgaatgaa tatttgcact attactattc aaaactatta agatagcaat
     2761 cacattcaat agccaaatac tataccacct gagcgatgca acgaaatgat caatttgagc
     2821 aaaaatgctg catatttagg acggcatcat tatagaaatg cttcttgctg tgtacttttc
     2881 tctcgtctgg cagctgtttc gccgttattg ttaaaaccgg cttaagttag gtgtgttttc
     2941 tacgactagt gaatgcccta ctagaagatg tgtgttgcac aaaatgtccc tggaataacc
     3001 aatttgaagt gcagatagca gtaaacgtaa gctaatatga atattattta actgtaatgt
     3061 tttaatatcg ctggacatta ctaataaacc cactataaac acatgtacat atgtatgttt
     3121 tggcatacaa tgagtagttg gggaaaaaat gtgtaaaagc accgtgacca tcacagcata
     3181 aagataacca gctgaagtat cgaatatgag taacccccaa attgaatcac atgccgcaac
     3241 tgataggacc catggaagta cactcttcat ggcgatatac aagacacaca caagcacgaa
     3301 cacccagttg cggaggaaat tctccgtaaa tgaaaaccca atcggcgaac aattcatacc
     3361 catatatggt aaaagttttg aacgcgactt gagagcggag agcattgcgg ctgataaggt
     3421 tttagcgcta agcgggcttt ataaaacggg ctgcgggacc agttttcata tcactaccgt
     3481 ttgagttctt gtgctgtgtg gatactcctc ccgacacaaa gccgctccat cagccagcag
     3541 tcgtctaatc cagagacccc ggatccgata agcttgatat cgaattcctg cagccctatg
     3601 aagtactgca agttctgctg caaggccgtg accggcgtga agctgatcca cgtgcccaag
     3661 tgcgccatca agcgcaagct gtgggagcag tccctgggct gctccctggg cgagaactcc
     3721 cagatctgcg acacccactt caacgactcc cagtggaagg ccgcccccgc caagggccag
     3781 accttcaagc gccgccgcct gaacgccgac gccgtgccct ccaaggtgat cgagcccgag
     3841 cccgagaaga tcaaggaggg ctacacctcc ggctccaccc agaccgagtc ctgctccctg
     3901 ttcaacgaga acaagtccct gcgcgagaag atccgcaccc tggagtacga gatgcgccgc
     3961 ctggagcagc agctgcgcga ggcccagcag ctggaggagt ccctgcgcaa gatcttcacc
     4021 gacacccaga tccgcatcct gaagaacggc ggccagcgcg ccaccttcaa ctccgacgac
     4081 atctccaccg ccatctgcct gcacaccgcc ggccctcgag cgtataacca tctgtacaaa
     4141 aaaggatttc ctttgcccag tcgtacgact ttgtacagat ggttatcaga tgtggacata
     4201 aaaagaggat gtttggatgt ggtcatagac ctaatggaca gtgatggagt tgatgacgcc
     4261 gacaagcttt gcgtactcgc tttcgacgag atgaaggtcg ctgctgcctt cgagtatgac
     4321 agctctgctg atattgttta cgagccaagc gactatgtcc aactggctat tgttcgtggt
     4381 ctaaaaaaat cgtggaagca gccagttttt ttcgatttta atacccgaat ggacccggat
     4441 actcttaaca atatattaag gaaactgcat aggaaaggat atttagtagt tgctattgta
     4501 tccgatttag gtaccggaaa ccaaaagcta tggacagagc tcggtatatc agaatcaaaa
     4561 acctggttta gccatcctgc agatgaccat ttaaagattt tcgttttttc ggatacgcca
     4621 catttaatta agttagtccg taaccactat gtggattccg gattaacaat aaatgggaaa
     4681 aaattaacaa aaaaaacaat tcaggaggca cttcatcttt gcaacaagtc cgatctgtct
     4741 atcctcttta aaattaatga aaatcacatt aatgttcgat cgctcgcaaa acagaaggtt
     4801 aaattggcta cccagctgtt ttcgaatacc accgctagct cgatcagacg ctgctattca
     4861 ttggggtatg acattgaaaa tgccaccgaa actgcggact tcttcaaatt gatgaatgat
     4921 tggttcgaca tttttaattc taaattgtcc acatccaatt gcattgagtg ctcgcaacct
     4981 tatggcaagc agttggatat acagaatgat attttgaatc gaatgtcgga aattatgcga
     5041 acaggaattc tggataaacc caaaaggctc ccatttcaaa aaggtatcat tgtgaataat
     5101 gcttcgcttg atggcttgta taaatatttg caagaaaact tcagtatgca atacatatta
     5161 acaagccgtc tcaaccaaga cattgtggag catttttttg gcagcatgcg atcgagaggt
     5221 ggacaattcg accatcccac tccactgcag tttaagtata ggttaagaaa atatataata
     5281 gccaggaata cagaaatgtt aagaaattcg ggaaatatcg aagaggacaa ctctgaaagc
     5341 tggcttaatt tagatttcag ttctaaagaa aacgaaaata aaagtaaaga tgatgagcct
     5401 gtcgatgatg agcctgtcga tgagatgtta agcaatatag atttcaccga aatggatgag
     5461 ttgacggagg atgcgatgga atatatcgcg ggctatgtca ttaaaaaatt gagaatcagt
     5521 gacaaagtaa aagaaaattt gacatttaca tacgtcgacg aggtgtctca cggcggactt
     5581 attaagccgt ccgaaaaatt tcaagagaag ttaaaagagc tagaatgtat ttttttgcat
     5641 tatacaaata ataataattt tgaaattaca aataatgtaa aggaaaaatt aatattagca
     5701 gcgcgaaacg tcgatgttga taaacaagta aaatcttttt attttaaaat tagaatatat
     5761 tttagaatta agtacttcaa caaaaaaatt gaaattaaaa atcaaaaaca aaagttaatt
     5821 ggaaactcca aattattaaa aataaaactt taaaaataat ttcgtctaat taatattatg
     5881 agttaattca aaccccacgg acatgctaag atccgcggcc gcctgcagct ggcgccatcg
     5941 atacgcgtac gtcgcgaccg cggacatgta cagagctcga gaagtactag tggccacgtg
     6001 ggccgtgcac cttaagcttg gcgcgcctag gccggccgaa ttcgaatggc catgggacgt
     6061 cgacctgagg taattataac ccgggcccta tatatggatc caattgcaat gatcatcatg
     6121 acagatctga caatgttcag tgcagagact cggctacgcc tcgtggactt tgaagttgac
     6181 caacaatgtt tattcttacc tctaatagtc ctctgtggca aggtcaagat tctgttagaa
     6241 gccaatgaag aacctggttg ttcaataaca ttttgttcgt ctaatatttc actaccgctt
     6301 gacgttggct gcacttcatg tacctcatct ataaacgctt cttctgtatc gctctggacg
     6361 tcatcttcac ttacgtgatc tgatatttca ctgtcagaat cctcaccaac aagctcgtca
     6421 tcgctttgca gaagagcaga gaggatatgc tcatcgtcta aagaactacc cattttatta
     6481 tatattagtc acgatatcta taacaagaaa atatatatat aataagttat cacgtaagta
     6541 gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat
     6601 aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc
     6661 attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc
     6721 gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt
     6781 acgcagacta tctttctagg gttaaaaaag atttgcgctt tactcgacct aaactttaaa
     6841 cacgtcatag aatcttcgtt tgacaaaaac cacattgtgg ccaagctgtg tgacgcgacg
     6901 cgcgctaaag aatggcaaac caagtcgcgc gagcgtcgac tctagaggat ccccgggtac
     6961 cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7021 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7081 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7141 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7201 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7261 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7321 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7381 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7441 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7501 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7561 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7621 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7681 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7741 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7801 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7861 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7921 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7981 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8041 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8101 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8161 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8221 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8281 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8341 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8401 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8461 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8521 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8581 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8641 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8701 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8761 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8821 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8881 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8941 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9001 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9061 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9121 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     9181 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9241 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9301 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     9361 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     9421 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     9481 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     9541 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     9601 caagctttgt ttaaaaatat aacaaaattg tgatcccaca aaatgaagtg gggcaaaatc
     9661 aaataattaa tagtgtccgt aaacttgttg gtcttcaact ttttgaggaa cacgttggac
     9721 ggcaaatccg tgactataac acaagttgat ttaataattt tagccaacac gtcgggctgc
     9781 gtgttttttg ccgacgcgtc tgtgtacacg ttgattaact ggtcgattaa actgttgaaa
     9841 taatttaatt tttggttctt ctttaaatct gtgatgaaat tttttaaaat aactttaaat
     9901 tcttcattgg taaaaaatgc cacgttttgc aacttgtgag ggtctaatat gaggtcaaac
     9961 tcagtaggag ttttatccaa aaaagaaaac atgattacgt ctgtacacga acgcgtatta
    10021 acgcagagtg caaagtataa gagggttaaa aaatatattt tacgcaccat atacgcatcg
    10081 ggttgatatc gttaatatgg atcaatttga acagttgatt aacgtgtctc tgctcaagtc
    10141 tttgatcaaa acgcaaatcg acgaaaatgt gtcggacaat atcaagtcga tgagcgaaaa
    10201 actaaaaagg ctagaatacg acaatctcac agacagcgtt gagatatacg gtattcacga
    10261 cagcaggctg aataataaaa aaattagaaa ctattattta accctagaaa gataatcata
    10321 ttgtgacgta cgttaaagat aatcatgcgt aaaattgacg catgtgtttt atcggtctgt
    10381 atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca
    10441 tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaaaca
    10501 aaaactcaaa atttcttcta taaagtaaca aaacttttaa acattctctc ttttacaaaa
    10561 ataaacttat tttgtacttt aaaaacagtc atgttgtatt ataaaataag taattagctt
    10621 aacttataca taatagaaac aaattatact tattagtcag tcagaaacaa ctttggcaca
    10681 tatcaatatt atgctctcga caaataactt ttttgcattt tttgcacgat gcatttgcct
    10741 ttcgccttat tttagagggg cagtaagtac agtaagtacg ttttttcatt actggctctt
    10801 cagtactgtc atctgatgta ccaggcactt catttggcaa aatattagag atattatcgc
    10861 gcaaatatct cttcaaagta ggagcttcta aacgcttacg cataaacgat gacgtcaggc
    10921 tcatgtaaag gtttctcata aattttttgc gactttgaac cttttctccc ttgctactga
    10981 cattatggct gtatataata aaagaattta tgcaggcaat gtttatcatt ccgtacaata
    11041 atgccatagg ccacctattc gtcttcctac tgcaggtcat cacagaacac atttggtcta
    11101 gcgtgtccac tccgccttta gtttgattat aatacataac catttgcggt ttaccggtac
    11161 tttcgttgat agaagcatcc tcatcacaag atgataataa gtataccatc ttagctggct
    11221 tcggtttata tgagacgaga gtaaggggtc cgtcaaaaca aaacatcgat gttcccactg
    11281 gcctggagcg actgtttttc agtacttccg gtatctcgcg tttgtttgat cgcacggttc
    11341 ccacaatggt taattcgagc tcgcccgggg atctaattca attagagact aattcaatta
    11401 gagctaattc aattaggatc caagcttatc gatttcgaac cctcgaccgc cggagtataa
    11461 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
    11521 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tcggggtacc
    11581 gctagagtcg acggtaccgc gggcccggga tccaccggtc gccacc
//
