LOCUS       sequence-180203-       11506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DsRed in eyes and ubiquitous expression of P-transposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Stern DL, Crocker J, Ding Y, Frankel N, Kappes G, Kim E, Kuzmickas 
            R, Lemire A, Mast JD, Picard S
  TITLE     Genetic and Transgenic Reagents for Drosophila simulans, D. 
            mauritiana, D. yakuba, D. santomea, and D. virilis.
  JOURNAL   G3 (Bethesda). 2017 Apr 3;7(4):1339-1347. doi: 
            10.1534/g3.116.038885.
  PUBMED    28280212
REFERENCE   2  (bases 1 to 11506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2017-04-3- 3"; volume: "7"; 
            issue: "4"; pages: "1339-1347"
COMMENT     Sequence Label: pBac{3XP3::DsRed, Pactin::Ptrsps}
FEATURES             Location/Qualifiers
     source          1..11506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     polyA_signal    5001..5122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5012..5031)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5066..5085
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(5243..5917,5918..5920,5921..5923))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(5305..5328)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5503..5522)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5720..5740
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      5920..5929
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   7186..7248
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7952..7969)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7952..7968)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7961..7983)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8177..8196)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8296..8318
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8356..8374)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8442..8546
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8547..8615,8616..9407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8765..8784)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9578..10166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10067..10086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10320..10337
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10454..10475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10490..10495,10496..10513,10514..10520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10528..10544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10533..10555
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10552..10568
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10552..10568
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   10749..10783
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatccg
     2521 ataagcttga tatcgaattc ctgcagccct atgaagtact gcaagttctg ctgcaaggcc
     2581 gtgaccggcg tgaagctgat ccacgtgccc aagtgcgcca tcaagcgcaa gctgtgggag
     2641 cagtccctgg gctgctccct gggcgagaac tcccagatct gcgacaccca cttcaacgac
     2701 tcccagtgga aggccgcccc cgccaagggc cagaccttca agcgccgccg cctgaacgcc
     2761 gacgccgtgc cctccaaggt gatcgagccc gagcccgaga agatcaagga gggctacacc
     2821 tccggctcca cccagaccga gtcctgctcc ctgttcaacg agaacaagtc cctgcgcgag
     2881 aagatccgca ccctggagta cgagatgcgc cgcctggagc agcagctgcg cgaggcccag
     2941 cagctggagg agtccctgcg caagatcttc accgacaccc agatccgcat cctgaagaac
     3001 ggcggccagc gcgccacctt caactccgac gacatctcca ccgccatctg cctgcacacc
     3061 gccggccctc gagcgtataa ccatctgtac aaaaaaggat ttcctttgcc cagtcgtacg
     3121 actttgtaca gatggttatc agatgtggac ataaaaagag gatgtttgga tgtggtcata
     3181 gacctaatgg acagtgatgg agttgatgac gccgacaagc tttgcgtact cgctttcgac
     3241 gagatgaagg tcgctgctgc cttcgagtat gacagctctg ctgatattgt ttacgagcca
     3301 agcgactatg tccaactggc tattgttcgt ggtctaaaaa aatcgtggaa gcagccagtt
     3361 tttttcgatt ttaatacccg aatggacccg gatactctta acaatatatt aaggaaactg
     3421 cataggaaag gatatttagt agttgctatt gtatccgatt taggtaccgg aaaccaaaag
     3481 ctatggacag agctcggtat atcagaatca aaaacctggt ttagccatcc tgcagatgac
     3541 catttaaaga ttttcgtttt ttcggatacg ccacatttaa ttaagttagt ccgtaaccac
     3601 tatgtggatt ccggattaac aataaatggg aaaaaattaa caaaaaaaac aattcaggag
     3661 gcacttcatc tttgcaacaa gtccgatctg tctatcctct ttaaaattaa tgaaaatcac
     3721 attaatgttc gatcgctcgc aaaacagaag gttaaattgg ctacccagct gttttcgaat
     3781 accaccgcta gctcgatcag acgctgctat tcattggggt atgacattga aaatgccacc
     3841 gaaactgcgg acttcttcaa attgatgaat gattggttcg acatttttaa ttctaaattg
     3901 tccacatcca attgcattga gtgctcgcaa ccttatggca agcagttgga tatacagaat
     3961 gatattttga atcgaatgtc ggaaattatg cgaacaggaa ttctggataa acccaaaagg
     4021 ctcccatttc aaaaaggtat cattgtgaat aatgcttcgc ttgatggctt gtataaatat
     4081 ttgcaagaaa acttcagtat gcaatacata ttaacaagcc gtctcaacca agacattgtg
     4141 gagcattttt ttggcagcat gcgatcgaga ggtggacaat tcgaccatcc cactccactg
     4201 cagtttaagt ataggttaag aaaatatata atagccagga atacagaaat gttaagaaat
     4261 tcgggaaata tcgaagagga caactctgaa agctggctta atttagattt cagttctaaa
     4321 gaaaacgaaa ataaaagtaa agatgatgag cctgtcgatg atgagcctgt cgatgagatg
     4381 ttaagcaata tagatttcac cgaaatggat gagttgacgg aggatgcgat ggaatatatc
     4441 gcgggctatg tcattaaaaa attgagaatc agtgacaaag taaaagaaaa tttgacattt
     4501 acatacgtcg acgaggtgtc tcacggcgga cttattaagc cgtccgaaaa atttcaagag
     4561 aagttaaaag agctagaatg tatttttttg cattatacaa ataataataa ttttgaaatt
     4621 acaaataatg taaaggaaaa attaatatta gcagcgcgaa acgtcgatgt tgataaacaa
     4681 gtaaaatctt tttattttaa aattagaata tattttagaa ttaagtactt caacaaaaaa
     4741 attgaaatta aaaatcaaaa acaaaagtta attggaaact ccaaattatt aaaaataaaa
     4801 ctttaaaaat aatttcgtct aattaatatt atgagttaat tcaaacccca cggacatgct
     4861 aagatccgcg gccgcctgca gctggcgcca tcgatacgcg tacgtcgcga ccgcggacat
     4921 gtacagagct cgagaagtac tagtggccac gtgggccgtg caccttaagc ttggcgcgcc
     4981 gtacgcgtat cgataagctt taagatacat tgatgagttt ggacaaacca caactagaat
     5041 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     5101 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     5161 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     5221 ttatgatcta gagtcgcggc cgctacagga acaggtggtg gcggccctcg gtgcgctcgt
     5281 actgctccac gatggtgtag tcctcgttgt gggaggtgat gtccagcttg gagtccacgt
     5341 agtagtagcc gggcagctgc acgggcttct tggccatgta gatggacttg aactccacca
     5401 ggtagtggcc gccgtccttc agcttcaggg ccttgtggat ctcgcccttc agcacgccgt
     5461 cgcgggggta caggcgctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg
     5521 ggccgtcgga ggggaagttc acgccgatga acttcacctt gtagatgaag cagccgtcct
     5581 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
     5641 cccacttgaa gccctcgggg aaggacagct tcttgtagtc ggggatgtcg gcggggtgct
     5701 tcacgtacac cttggagccg tactggaact ggggggacag gatgtcccag gcgaagggca
     5761 gggggccgcc cttggtcacc ttcagcttca cggtgttgtg gccctcgtag gggcggccct
     5821 cgccctcgcc ctcgatctcg aactcgtggc cgttcacggt gccctccatg cgcaccttga
     5881 agcgcatgaa ctccttgatg acgttcttgg aggagcgcac catggtggcg accggtggat
     5941 cccgggcccg cggtaccgtc gactctagcg gtaccccgat tgtttagctt gttcagctgc
     6001 gcttgtttat ttgcttagct ttcgcttagc gacgtgttca ctttgcttgt ttgaattgaa
     6061 ttgtcgctcc gtagacgaag cgcctctatt tatactccgg cggtcgaggg ttcgaaatcg
     6121 ataagcttgg atcctaattg aattagctct aattgaatta gtctctaatt gaattagatc
     6181 cccgggcgag ctcgaattaa ccattgtggg aaccgtgcga tcaaacaaac gcgagatacc
     6241 ggaagtactg aaaaacagtc gctccaggcc agtgggaaca tcgatgtttt gttttgacgg
     6301 accccttact ctcgtctcat ataaaccgaa gccagctaag atggtatact tattatcatc
     6361 ttgtgatgag gatgcttcta tcaacgaaag taccggtaaa ccgcaaatgg ttatgtatta
     6421 taatcaaact aaaggcggag tggacacgct agaccaaatg tgttctgtga tgacctgcag
     6481 taggaagacg aataggtggc ctatggcatt attgtacgga atgataaaca ttgcctgcat
     6541 aaattctttt attatataca gccataatgt cagtagcaag ggagaaaagg ttcaaagtcg
     6601 caaaaaattt atgagaaacc tttacatgag cctgacgtca tcgtttatgc gtaagcgttt
     6661 agaagctcct actttgaaga gatatttgcg cgataatatc tctaatattt tgccaaatga
     6721 agtgcctggt acatcagatg acagtactga agagccagta atgaaaaaac gtacttactg
     6781 tacttactgc ccctctaaaa taaggcgaaa ggcaaatgca tcgtgcaaaa aatgcaaaaa
     6841 agttatttgt cgagagcata atattgatat gtgccaaagt tgtttctgac tgactaataa
     6901 gtataatttg tttctattat gtataagtta agctaattac ttattttata atacaacatg
     6961 actgttttta aagtacaaaa taagtttatt tttgtaaaag agagaatgtt taaaagtttt
     7021 gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa
     7081 taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc
     7141 tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta
     7201 cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt aaataatagt
     7261 ttctaatttt tttattattc agcctgctgt cgtgaatacc gtatatctca acgctgtctg
     7321 tgagattgtc gtattctagc ctttttagtt tttcgctcat cgacttgata ttgtccgaca
     7381 cattttcgtc gatttgcgtt ttgatcaaag acttgagcag agacacgtta atcaactgtt
     7441 caaattgatc catattaacg atatcaaccc gatgcgtata tggtgcgtaa aatatatttt
     7501 ttaaccctct tatactttgc actctgcgtt aatacgcgtt cgtgtacaga cgtaatcatg
     7561 ttttcttttt tggataaaac tcctactgag tttgacctca tattagaccc tcacaagttg
     7621 caaaacgtgg cattttttac caatgaagaa tttaaagtta ttttaaaaaa tttcatcaca
     7681 gatttaaaga agaaccaaaa attaaattat ttcaacagtt taatcgacca gttaatcaac
     7741 gtgtacacag acgcgtcggc aaaaaacacg cagcccgacg tgttggctaa aattattaaa
     7801 tcaacttgtg ttatagtcac ggatttgccg tccaacgtgt tcctcaaaaa gttgaagacc
     7861 aacaagttta cggacactat taattatttg attttgcccc acttcatttt gtgggatcac
     7921 aattttgtta tatttttaaa caaagcttgg cactggccgt cgttttacaa cgtcgtgact
     7981 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     8041 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     8101 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8161 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     8221 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     8281 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     8341 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     8401 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8461 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8521 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8581 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8641 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8701 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8761 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8821 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8881 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8941 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9001 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9061 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9121 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9181 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9241 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9301 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9361 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9421 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9481 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9541 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9601 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9661 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9721 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9781 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9961 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10141 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10201 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10261 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10321 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10381 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10441 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10501 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10561 gctatgacat gattacgaat tcgagctcgg tacccgggga tcctctagag tcgacgctcg
    10621 cgcgacttgg tttgccattc tttagcgcgc gtcgcgtcac acagcttggc cacaatgtgg
    10681 tttttgtcaa acgaagattc tatgacgtgt ttaaagttta ggtcgagtaa agcgcaaatc
    10741 ttttttaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc
    10801 tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct
    10861 tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac
    10921 gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca
    10981 tacgataatt atattgttat ttcatgttct acttacgtga taacttatta tatatatatt
    11041 ttcttgttat agatatcgtg actaatatat aataaaatgg gtagttcttt agacgatgag
    11101 catatcctct ctgctcttct gcaaagcgat gacgagcttg ttggtgagga ttctgacagt
    11161 gaaatatcag atcacgtaag tgaagatgac gtccagagcg atacagaaga agcgtttata
    11221 gatgaggtac atgaagtgca gccaacgtca agcggtagtg aaatattaga cgaacaaaat
    11281 gttattgaac aaccaggttc ttcattggct tctaacagaa tcttgacctt gccacagagg
    11341 actattagag gtaagaataa acattgttgg tcaacttcaa agtccacgag gcgtagccga
    11401 gtctctgcac tgaacattgt cagatccgag atcggccggc ctaggcgcgc ctaggccggc
    11461 cgaattctat attctaaaaa cacaaatgat acttctaaaa aaaaat
//
