LOCUS       sequence-180213-       12760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For constitutive expression of firefly luciferase from the CAG 
            promoter together with an IRES-linked neo resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, 
            Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, 
            Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis
            PL, Jeffree RL, Johns TG, Boyd AW
  TITLE     A reference collection of patient-derived cell line and xenograft 
            models of proneural, classical and mesenchymal glioblastoma.
  JOURNAL   Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
  PUBMED    30894629
REFERENCE   2  (bases 1 to 12760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-03-20"; volume: "9(1):4902. doi"; pages: " 
            10.1038/s41598-019-41277-z"
COMMENT     Sequence Label: pF CAG luc IRES neo
FEATURES             Location/Qualifiers
     source          1..12760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    501..622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(538..557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     592..611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(671..687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(671..687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(684..706)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    695..711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(719..725,726..743,744..749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    764..785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(902..919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1073..1661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1153..1172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1821..2612,2613..2681))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2444..2463
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(2857..2876)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3048..3427
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3429..3627
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3582..3602
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3645..3825
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3872..3997
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4490..4723
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4908..4952
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5250..5367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        5475..5778
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5781..6056
                     /label=chicken beta-actin promoter
     intron          6057..7065
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6989..7011
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      7123..7132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7129..8781
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(7195..7213)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    8789..9362
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8812..9364
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8956..8973)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9183..9202
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9427..10221
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9481..9500)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10091..10110
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    10278..10866
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10331..10351)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10749..10760)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10869..10885)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10870..10886)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11390..11570
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11596..11613)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11602..11826
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11872..12300
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11959..11978)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12169..12190
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12309..12329)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12314..12643
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12494..12629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12556..12575
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12691..12738
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(12757..12760,1..371)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
       61 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
      121 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
      181 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
      241 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
      301 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
      361 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
      421 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
      481 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
      541 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      601 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
      661 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      721 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1801 aaagtatata tgagtaaact ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1861 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1921 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1981 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2041 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2101 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2161 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2221 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2281 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2341 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2401 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2461 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2521 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2581 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2641 aaaaagggaa taagggcgac acggaaatgt tgaatactca tctcttcttt ttatattatt
     2701 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2761 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     2821 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     2881 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     2941 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     3001 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     3061 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     3121 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     3181 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     3241 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     3301 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     3361 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     3421 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     3481 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     3541 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     3601 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     3661 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3721 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3781 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     3841 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     3901 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     3961 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     4021 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     4081 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     4141 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     4201 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     4261 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     4321 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     4381 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     4441 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     4501 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     4561 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     4621 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     4681 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     4741 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     4801 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     4861 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     4921 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     4981 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     5041 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     5101 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     5161 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     5221 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     5281 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     5341 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     5401 tggttaatta agactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     5461 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     5521 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     5581 ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc agtacatcaa
     5641 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     5701 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     5761 gtcatcgcta ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     5821 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     5881 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
     5941 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
     6001 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
     6061 tcgctgcgtt gccttcgccc cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc
     6121 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     6181 gtaattagcg cttggtttaa tgacggctcg tttcttttct gtggctgcgt gaaagcctta
     6241 aagggctccg ggagggccct ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt
     6301 gtgtgcgtgg ggagcgccgc gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc
     6361 gcggcgcggg gctttgtgcg ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc
     6421 cccgcggtgc gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     6481 gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac ccccccctgc acccccctcc
     6541 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg
     6601 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
     6661 tcgggccggg gagggctcgg gggaggggcg cggcggcccc ggagcgccgg cggctgtcga
     6721 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
     6781 cctttgtccc aaatctggcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     6841 ggcgcgggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     6901 gtcgccgcgc cgccgtcccc ttctccatct ccagcctcgg ggctgccgca gggggacggc
     6961 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     7021 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     7081 ctggttgttg tgctgtctca tcattttggc aaaggatccg ccgccaccat ggaagacgcc
     7141 aaaaacataa agaaaggccc ggcgccattc tatcctctag aggatggaac cgctggagag
     7201 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     7261 gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca
     7321 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     7381 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     7441 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc
     7501 gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca
     7561 ataatccaga aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     7621 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag
     7681 tcctttgatc gtgacaaaac aattgcactg ataatgaatt cctctggatc tactgggtta
     7741 cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat
     7801 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     7861 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     7921 atgtatagat ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaaagt
     7981 gcgttgctag taccaaccct attttcattc ttcgccaaaa gcactctgat tgacaaatac
     8041 gatttatcta atttacacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg
     8101 gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag
     8161 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     8221 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     8281 aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggtta tgtaaacaat
     8341 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     8401 tactgggacg aagacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac
     8461 aaaggatatc aggtggcccc cgctgaattg gaatcgatat tgttacaaca ccccaacatc
     8521 ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     8581 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     8641 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     8701 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     8761 ggcggaaagt ccaaattgta agctagcgcc cctctccctc ccccccccct aacgttactg
     8821 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     8881 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     8941 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     9001 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     9061 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     9121 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     9181 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     9241 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     9301 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     9361 aatatggcca caaaccggtc ttgattcttc tgacacaaca gtctcgaact taaggctaga
     9421 gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     9481 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     9541 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     9601 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     9661 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     9721 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     9781 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     9841 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     9901 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     9961 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
    10021 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
    10081 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
    10141 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
    10201 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaggcgc gccgaattcg
    10261 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
    10321 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
    10381 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
    10441 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
    10501 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
    10561 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
    10621 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
    10681 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
    10741 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
    10801 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
    10861 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
    10921 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
    10981 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
    11041 ttagccactt tttaaaagaa aaggggggac tggagggcta attcactccc aacgaagaca
    11101 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
    11161 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
    11221 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
    11281 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
    11341 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
    11401 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    11461 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    11521 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
    11581 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
    11641 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
    11701 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
    11761 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
    11821 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
    11881 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    11941 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    12001 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    12061 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    12121 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    12181 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    12241 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    12301 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    12361 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    12421 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    12481 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    12541 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
    12601 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
    12661 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
    12721 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
//
