LOCUS       sequence-180215-       11640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one mouse super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 11640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl G
FEATURES             Location/Qualifiers
     source          1..11640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          2413..2985
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3059..3068
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3065..3067,3068..3070,3071..3784)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3065..3067,3068..3070,3071..3781)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3110..3131)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3371..3390)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3718..3739
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3829..3963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3853..3872)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3907..3926
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(4400..4587,4588..4599)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4633..4649)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4633..4649)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4686..4706)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4686..4704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4725..4741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4725..4741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4738..4760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4749..4765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4773..4779,4780..4797,4798..4803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4818..4839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4956..4973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5127..5715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5207..5226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5886..6677,6678..6746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6509..6528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6747..6851)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6877..7332)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7014..7035)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7226..7245
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7459..7481
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7473..7490
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7474..7490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7500..7519
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7500..7518
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(9370..9381,9382..9544)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    9923..10047
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(10072..10077,10078..10095,10096..10102)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             10156..10815
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10222..10241)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             11157..11462
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11341..11360
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(11503..11627)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggactcgaat ttcagcaggt gagagaaatc aaggcaacag agcccagtgt ggctaaggtc
       61 acacactaag gtcgggctgc agggacagag ctaaggacag gcttcagaca gacccacaac
      121 caaggcccca gcccagtaca gaggaggagc aaacatttcc cagcttgtgt ctgaagtaga
      181 ccccagcact ttccacaccc gagaccttga ggcagcggct gtcctttctt ctggggaaat
      241 agaggccctg gggttcccct ggagccccct tggcaaatta ggtccagacc aggcaaagct
      301 ctccaatgac tcaactccca gggaggcctt gaaacaccag accagacatt taaaaaaaag
      361 gaaagaaaga aaaagaaaga aagaaagaaa aaaaaaaaac tggcaggatt caagagaagg
      421 gattagatca ttgcaacagg atgaaggaaa tgaggaacct agggagggga ggagggacag
      481 gggccaagaa tgtctcaaga caaccccaag cgcctacaga ttaaagcagg ctcgtgcacc
      541 aacagctgag gccactgcca ggaagtcctg gcctcattca gcccctttag ggcggggtaa
      601 aaataatctc gtccatacac aaggagatca cacactcaca gatcagcaac caaatcccgg
      661 ggaggctgtg agcacggtaa agacgtttgg gcaccctatg tgtacagaca aaattcagcc
      721 acacagacac atgtgtgcag aagtccgggg cctggcgtgc aagcccacgg gactgtgtgt
      781 gtgtgtgtgt ttgcgtgcag tctacaaaca tgttttcaca aagctctggg gagaagcgat
      841 cattcatctc ccaggaatct tagcaacagc catccccaga aaaggctctg ccagctggag
      901 cggggatgag tcagcagagg gctggtgggg gaggggcagc tccgcccctg gcctggcctc
      961 ccccttctcc tgggaaggtc tccaggccta gcctggccct ggctgctgct cctgggtcac
     1021 tgccttggca ctgggggccg agaacacatt cctggaaccg gccagcacag acggctagag
     1081 ggttttccct cctcctgctt ccccctccag tcccgcccca cacccgccct tttatttttc
     1141 cttctcgctg cagaaccaaa acaccgtgtt cacgagccct ggaatgggag ccaaatggca
     1201 agcatagagc gattggacac aggagctgcc aggcccaagc aggccaggca atgaggacac
     1261 ctcctgtcag cagcccaggg cccagcgagg ggctgtcata ctctccttcc atctatcccg
     1321 gcaaagggtc attgctcagg gctcctggga actggagttc aaggtgatgg ctgctgggga
     1381 gctaggctgt gtcactcagc ctggatagct gaggctgagg ttgctaacac gggaggggag
     1441 ggtgaccgga ggaacagcag attcatcatg acatcattgc ctgagagcat caagttcacc
     1501 ttcactgcgt gctcaggagt ggcagaaccg tctttttttc agatggggag atgaagatgc
     1561 aagacgggag aggcctgggg cccaaggtca tctgcagaga caagccccag gcgctagatg
     1621 aagtgaaagc aggctacagc cgacttggat taaggacagg tagcccttgc tagttcccca
     1681 ggctccacct acccctagtg tgccaggtcc atacaaaaac aaacagaata aaaaacaaaa
     1741 caaagagcta gcgaaatctc atccctgtcc ccaaacggat ctcaacgagg agccaaaggc
     1801 ggcggcggct ctgcagcctt ggtgaaggtt gtcgtgagca ggggcagtaa ccacccggcc
     1861 cggcccaaat ccctggcctc tttactggag gcaggaaatg aataaaaagc taaatgagtt
     1921 tggaaatgcc aggaggcagt gtttatcccc cttcccccac cccctcccct tggcaaggac
     1981 cgtgtaaagt tacagtttaa tcttggaaat gccagacagc tgttccccgt tgctaagaga
     2041 ctggcccaca ttatgcagat gtcagagacc tcaaatgtct gccggaaatt actgatcatg
     2101 ttggaagagc agaggggcct tctctcggcc cgggaagcgc tcagcaggag accccctggg
     2161 gatagcagaa ataaggagtc cacccctccc tgcctcttcc tcagattctc tggaaccttg
     2221 gcttctgggc tagtcctgac tcatggcccc tttctccttt ctgtctcctc ctatacccct
     2281 gcctctatcc ttccaggaca atgtacaccg acagatctct cgactctaga gggtatataa
     2341 tggatcccat cgcgtctcag cctcactttg agctcctcca cacgaatttc gaccgatcct
     2401 gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa
     2461 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc
     2521 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa
     2581 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg
     2641 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc
     2701 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag
     2761 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg
     2821 cgtggaaata ttcttattgg tagaaacaac tacaccctgg tcatcatcct gcctttctct
     2881 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg
     2941 ggcccctctg ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg
     3001 ctggttgttg tgctgtctca tcattttggc aaagaattcc tcgacggatc caccggtcgc
     3061 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3121 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3181 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3241 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     3301 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     3361 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     3421 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3481 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3541 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3601 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3661 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3721 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3781 gtaaagcggc cgccaccgcg gtggagctcg aattaattca tcgatgatga tccagacatg
     3841 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3901 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3961 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     4021 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcag
     4081 atccgaagat acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc
     4141 gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt
     4201 tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact
     4261 tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag
     4321 gcagaataaa taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat
     4381 agcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa
     4441 aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa
     4501 gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac
     4561 agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca
     4621 gcaagcccag cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt
     4681 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     4741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6841 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     6901 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     6961 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     7021 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     7081 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     7141 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     7201 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     7261 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     7321 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     7381 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     7441 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     7501 aatacgactc actatagggc gaattgggta ccaaatagta ggaattaccc acctgtacaa
     7561 gtgctgaaaa cttggatgaa taagcccgtt tgccattttc tactgctatt ttaaatcttt
     7621 tctgtatttg tctgcatttg tctttaccct tgaataaatg ttttagttgt tttttttcaa
     7681 ttatgactgt gtttaacaga cattattaca ataatatgta atagtagtac acactattat
     7741 tatgtaatag tacttgttga ctgtatttga gaactggacc gagtgagtgt tacgtcaccc
     7801 attcaaaatg acttacttct ggctccaaca aaatgaagtt aattcagttg ccatttttca
     7861 ctgtatggac atcgccgtgt tggagctaga cgttgccaat aagcaagaaa gagccgagat
     7921 gcgtcgagtc tgagtcaccg ttcctatggc aacccctcta accaatcaga agtaagcttg
     7981 ttggaagtcc acagcctacc acttgaaagc gggctgcaca aaatctgtca aacgttttga
     8041 acgttggatg tgagagcaca tacttttatt aaggcatctg gttggtcagt ttataatatc
     8101 aacaacttgg gctacagaaa agaaaagtta ttacagaaat tatgattaac aagtacatgt
     8161 taaataaaga ttttaatatg aatgccacca ctggagcatt catgccattt ggagcttctt
     8221 cctgtttgga tcactagaag gaggaggtca ctcattacag ttctcatata cagtcgttgg
     8281 ttggttggtt ggttggtaga ttgattgatt gattgattga ttgattgatt gattgattga
     8341 ttgattgatt gattgattga ttgattgatt gattggtagt caaaataaga aataatttcc
     8401 acagattcat tacagaaatg attaaatgca tacataaaaa actggggggg gggggggata
     8461 caacaacaca cttaagtcac atttgcctac gtaaagaaaa gtaaagaaaa tcaatagcta
     8521 tattttacat ctcctttttt tgctgtcttt taattagcct tgttttgctg ttatcttgat
     8581 tgaaactgta acttcttcac ctgcttcttt tctttgtagg ttttgccagc ccagaaacag
     8641 gcatggctgt gcaaggccag agcaccatgc acaatgcgct gcatgtcttc atgaacggct
     8701 caatgtcctc agtccagggc tcagccaacg accccatctt cctccttcac catgctttca
     8761 ttgacaggta acaaacacgt catgacatta gactgcacag tttttgacaa agttcataca
     8821 atctgttgtt tatagctgct acaattagtg aagtttgtga atgtacttgg atgagcagcg
     8881 aaagatcaat tgagatcaat tgttagagtt tggttgccct gcagagcaaa gaacaaaaaa
     8941 taatctggtg gctttactgc gtgaggttat tattggtgga atagaaacac aaaacataat
     9001 tgcatttatt tgtttaattt tttatcttat cttaactttc atcttgcata tttgtttctt
     9061 acatcatttc tagcatcttt gagcgctggc taagaactca tcagcctccc cggtccatct
     9121 acccacgtac caatgcacca attggccaca atgacggcta ctacatggtg ccattccttc
     9181 ctctttatag gaatggagac tacctcctgt ccaacaatgc tcttggatac gagtacgcct
     9241 acctgttgga cccaggtcat tgcacaacac cagaaatgcc ctctgatctg caaaagacgt
     9301 gaatatctgt tcagacaccc atatccactc tgttccacac aggtcagagg tttgtccagg
     9361 agttcttgac agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac
     9421 ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa
     9481 aagtaaaaaa gtactccatt aaaattgtac ttgagtatta aggaagtaaa agtaaaagca
     9541 agaaagaaaa ctagagattc ttgtttaagc ttttaatctc aaaaaacatt aaatgaaatg
     9601 catacaaggt tttatcctgc tttagaactg tttgtattta attatcaaac tataagacag
     9661 acaatctaat gccagtacac gctactcaaa gttgtaaaac ctcagattta acttcagtag
     9721 aagctgattc tcaaaattgt tagtgtcaag cctagctctt ttggggctga aaagcaatcc
     9781 tgcagtgctg aaaagcctct cacaggcagc cgatgcggga agaggtgtat tagtcttgat
     9841 agagaggctg caaatagcag gaaacgtgag cagagactcc ctggtgtctg aaacacaggc
     9901 cagatgggcc ctcgaggata tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
     9961 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
    10021 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
    10081 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
    10141 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
    10201 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
    10261 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
    10321 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
    10381 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
    10441 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
    10501 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
    10561 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
    10621 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
    10681 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
    10741 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
    10801 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
    10861 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
    10921 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
    10981 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
    11041 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
    11101 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
    11161 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
    11221 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
    11281 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
    11341 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
    11401 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
    11461 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
    11521 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
    11581 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat atcaagatct
//
