LOCUS       sequence-180216-        9862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ADSL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: ADSL_pLX307
FEATURES             Location/Qualifiers
     source          1..9862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2773..2797)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2805..2846
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2897..3485
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2950..2970)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3368..3379)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3557..3790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3868..3989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3905..3924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3959..3978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4029..4164
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4184..4203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4185..4203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4213..4230)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4213..4229)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4222..4244)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4371..4826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4458..4477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4668..4689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4852..4956
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4957..5025,5026..5817)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5175..5194)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5988..6576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6477..6496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6730..6747
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6864..6885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6900..6905,6906..6923,6924..6930)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6938..6954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6943..6965
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6962..6978
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6962..6978
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6997..7017
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6999..7017
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7045..7271
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7272..7452
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7499..7624
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8117..8350
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8535..8579
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8810..9020
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8832..9028
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8879..9014
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9060..9662
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9060..9079)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9556..9576
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9705..9822
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggcggctgg aggcgatcat ggttcgcccg acagctaccg ctcacctctt gcctcccgct
     1381 atgccagccc ggagatgtgc ttcgtgttta gcgacaggta taaattccgg acatggcggc
     1441 agctgtggct gtggctggcg gaggccgagc agacattggg tttgcctatc acagatgaac
     1501 aaatccagga gatgaaatca aacctggaga acatcgactt caagatggca gctgaggaag
     1561 agaaacgttt acgacatgat gtgatggctc acgtgcacac atttggccac tgctgtccaa
     1621 aagctgcagg cattattcac cttggtgcta cttcttgcta tgttggagac aatactgact
     1681 tgattattct tagaaatgca cttgacctgc ttttgccaaa gcttgccaga gtgatctctc
     1741 ggcttgccga ctttgctaag gaacgagcca gtctacccac attaggtttc acacatttcc
     1801 agcctgcaca gctgaccaca gttgggaaac gttgctgtct ttggattcag gatctttgca
     1861 tggatctcca gaacttgaag cgtgtccgag atgacctgcg cttccgggga gtaaagggta
     1921 ccactggcac tcaggccagt ttcctgcagc tctttgaggg agatgaccat aaggtagagc
     1981 agcttgacaa gatggtgaca gaaaaggcag gatttaagag agctttcatc atcacagggc
     2041 agacatatac acgaaaagtg gatattgaag tactgtctgt gctggctagc ttgggggcat
     2101 cagtgcacaa gatttgcacc gacatacgcc tcctggcaaa cctcaaggag atggaggaac
     2161 cctttgaaaa acagcagatt ggctcaagtg cgatgccata taagcggaat cccatgcgtt
     2221 cagaacgttg ctgcagtctt gcccgccacc tgatgaccct tgtcatggac ccgctacaga
     2281 cagcatctgt ccagtggttt gaacgcacac tggatgatag tgccaaccga cggatctgtt
     2341 tggccgaggc atttcttacc gcagatacta tattgaatac gctgcagaac atttctgaag
     2401 gattggtcgt gtaccccaaa gtaattgaac ggcgcattcg gcaagagctg cctttcatgg
     2461 ccacagagaa catcatcatg gccatggtca aagctggagg tagccgccag gattgccatg
     2521 agaaaatcag agtgctttct cagcaggcag cttctgtggt taagcaggaa gggggtgaca
     2581 atgacctcat agagcgtatc caggttgatg cctacttcag tcccattcac tcccagttgg
     2641 atcatttact ggatccttct tctttcactg gtcgtgcctc ccagcaggtg cagagattct
     2701 tagaagagga ggtgtatccc ctgttaaaac catatgaaag cgtgatgaag gtgaaagcag
     2761 aattatgtct gtacccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta
     2821 accctctcct cggtctcgat tctacgtagt aatgaactag taccggttaa gtcgacaatc
     2881 aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2941 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3001 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3061 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3121 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3181 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3241 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3301 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3361 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3421 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3481 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3541 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     3601 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3781 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     3841 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     3901 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3961 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     4021 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4081 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4141 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     4201 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4261 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4321 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     4381 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4441 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4501 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4561 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4621 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4681 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4741 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4801 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     4861 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4921 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4981 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5041 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5101 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5161 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5221 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5281 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5341 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5401 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5461 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5521 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5581 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5641 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5701 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5761 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5821 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5881 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5941 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6001 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6061 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6121 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6181 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6241 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6301 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6361 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6421 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6481 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6541 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6601 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6661 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6721 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6781 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6841 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     6901 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     6961 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     7021 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     7081 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     7141 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     7201 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     7261 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7321 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7381 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7441 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     7501 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     7561 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     7621 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     7681 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     7741 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     7801 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     7861 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     7921 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     7981 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     8041 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     8101 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     8161 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     8221 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     8281 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     8341 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     8401 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     8461 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     8521 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     8581 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     8641 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     8701 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt
     8761 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8821 tatgcaaagc atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8881 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8941 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9001 cttttttgga ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca
     9061 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     9121 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     9181 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     9241 tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga
     9301 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     9361 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     9421 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     9481 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     9541 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     9601 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt
     9661 aacgaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     9721 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9781 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9841 agcagagatc cactttggcg cc
//
