LOCUS       sequence-180225-       10009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BMPR1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: BMPR1A_pLX307
FEATURES             Location/Qualifiers
     source          1..10009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2920..2944)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2952..2993
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3044..3632
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3097..3117)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3515..3526)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3704..3937
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4015..4136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4052..4071)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4106..4125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4176..4311
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4331..4350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4332..4350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4360..4377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4360..4376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4369..4391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4518..4973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4605..4624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4815..4836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4999..5103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5104..5172,5173..5964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5322..5341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6135..6723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6624..6643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6877..6894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7011..7032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7047..7052,7053..7070,7071..7077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7085..7101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7090..7112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7109..7125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7109..7125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7144..7164
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7146..7164
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7192..7418
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7419..7599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7646..7771
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8264..8497
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8682..8726
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8957..9167
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8979..9175
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9026..9161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9207..9809
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9207..9226)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9703..9723
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9852..9969
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgcctcagct atacatttac atcagattat tgggagccta tttgttcatc atttctcgtg
     1381 ttcaaggaca gaatctggat agtatgcttc atggcactgg gatgaaatca gactccgacc
     1441 agaaaaagtc agaaaatgga gtaaccttag caccagagga taccttgcct tttttaaagt
     1501 gctattgctc agggcactgt ccagatgatg ctattaataa cacatgcata actaatggac
     1561 attgctttgc catcatagaa gaagatgacc agggagaaac cacattagct tcagggtgta
     1621 tgaaatatga aggatctgat tttcagtgca aagattctcc aaaagcccag ctacgccgga
     1681 caatagaatg ttgtcggacc aatttatgta accagtattt gcaacccaca ctgccccctg
     1741 ttgtcatagg tccgtttttt gatggcagca ttcgatggct ggttttgctc atttctatgg
     1801 ctgtctgcat aattgctatg atcatcttct ccagctgctt ttgttacaaa cattattgca
     1861 agagcatctc aagcagacgt cgttacaatc gtgatttgga acaggatgaa gcatttattc
     1921 cagttggaga atcactaaaa gaccttattg accagtcaca aagttctggt agtgggtctg
     1981 gactaccttt attggttcag cgaactattg ccaaacagat tcagatggtc cggcaagttg
     2041 gtaaaggccg atatggagaa gtatggatgg gcaaatggcg tggcgaaaaa gtggcggtga
     2101 aagtattctt taccactgaa gaagccagct ggtttcgaga aacagaaatc taccaaactg
     2161 tgctaatgcg ccatgaaaac atacttggtt tcatagcggc agacattaaa ggtacaggtt
     2221 cctggactca gctctatttg attactgatt accatgaaaa tggatctctc tatgacttcc
     2281 tgaaatgtgc tacactggac accagagccc tgcttaaatt ggcttattca gctgcctgtg
     2341 gtctgtgcca cctgcacaca gaaatttatg gcacccaagg aaagcccgca attgctcatc
     2401 gagacctaaa gagcaaaaac atcctcatca agaaaaatgg gagttgctgc attgctgacc
     2461 tgggccttgc tgttaaattc aacagtgaca caaatgaagt tgatgtgccc ttgaatacca
     2521 gggtgggcac caaacgctac atggctcccg aagtgctgga cgaaagcctg aacaaaaacc
     2581 acttccagcc ctacatcatg gctgacatct acagcttcgg cctaatcatt tgggagatgg
     2641 ctcgtcgttg tatcacagga gggatcgtgg aagaatacca attgccatat tacaacatgg
     2701 taccgagtga tccgtcatac gaagatatgc gtgaggttgt gtgtgtcaaa cgtttgcggc
     2761 caattgtgtc taatcggtgg aacagtgatg aatgtctacg agcagttttg aagctaatgt
     2821 cagaatgctg ggcccacaat ccagcctcca gactcacagc attgagaatt aagaagacgc
     2881 ttgccaagat ggttgaatcc caagatgtaa aaatcagacc cccaactttc ttgtacaaag
     2941 tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
     3001 gaactagtac cggttaagtc gacaatcaac gcgttaagtc gacaatcaac ctctggatta
     3061 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3121 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3181 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3241 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3301 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3361 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3421 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3481 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3541 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3601 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     3661 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     3721 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     3781 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     3841 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     3901 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     3961 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     4021 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4081 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4141 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4201 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4261 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     4321 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     4381 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4441 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4501 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     4981 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5041 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5101 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5161 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5221 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5281 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5341 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5401 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5461 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5521 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5581 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5641 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5701 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5761 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5821 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5881 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5941 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6001 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6061 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6241 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6541 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6781 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6841 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6901 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6961 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7021 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7081 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7141 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     7201 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     7261 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     7321 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     7381 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     7441 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7501 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7561 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7621 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7681 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7741 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7801 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7861 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7921 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     7981 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     8041 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     8101 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     8161 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     8221 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     8281 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     8341 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     8401 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     8461 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     8521 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     8581 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     8641 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     8701 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     8761 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     8821 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     8881 tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa
     8941 gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac
     9001 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9061 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9121 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9181 ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     9241 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     9301 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     9361 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     9421 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     9481 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     9541 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     9601 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     9661 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     9721 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     9781 gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta
     9841 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     9901 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     9961 caaaattttc gggtttatta cagggacagc agagatccac tttggcgcc
//
