LOCUS       sequence-180240-        9337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GPR35.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: GPR35_pLX307
FEATURES             Location/Qualifiers
     source          1..9337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2248..2272)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2280..2321
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2372..2960
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2425..2445)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2843..2854)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3032..3265
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3343..3464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3380..3399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3434..3453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3504..3639
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3659..3678)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3660..3678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3688..3705)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3688..3704)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3697..3719)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3846..4301
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3933..3952)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4143..4164
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4327..4431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4432..4500,4501..5292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5463..6051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5952..5971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6205..6222
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6339..6360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6375..6380,6381..6398,6399..6405)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6413..6429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6418..6440
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6437..6453
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6437..6453
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6472..6492
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6474..6492
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6520..6746
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6747..6927
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6974..7099
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7592..7825
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8010..8054
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8285..8495
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8307..8503
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8354..8489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8535..9137
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8535..8554)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9031..9051
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9180..9297
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgaatggcac ctacaacacc tgtggctcca gcgacctcac ctggccccca gcgatcaagc
     1381 tgggcttcta cgcctacttg ggcgtcctgc tggtgctagg cctgctgctc aacagcctgg
     1441 cgctctgggt gttctgctgc cgcatgcagc agtggacgga gacccgcatc tacatgacca
     1501 acctggcggt ggccgacctc tgcctgctgt gcaccttgcc cttcgtgctg cactccctgc
     1561 gagacacctc agacacgccg ctgtgccagc tctcccaggg catctacctg accaacaggt
     1621 acatgagcat cagcctggtc acggccatcg ccgtggaccg ctatgtggcc gtgcggcacc
     1681 cgctgcgtgc ccgcgggctg cggtccccca ggcaggctgc ggccgtgtgc gcggtcctct
     1741 gggtgctggt catcggctcc ctggtggctc gctggctcct ggggattcag gagggcggct
     1801 tctgcttcag gagcacccgg cacaatttca actccatggc gttcccgctg ctgggattct
     1861 acctgcccct ggccgtggtg gtcttctgct ccctgaaggt ggtgactgcc ctggcccaga
     1921 ggccacccac cgacgtgggg caggcagagg ccacccgcaa ggctgcccgc atggtctggg
     1981 ccaacctcct ggtgttcgtg gtctgcttcc tgcccctgca cgtggggctg acagtgcgcc
     2041 tcgcagtggg ctggaacgcc tgtgccctcc tggagacgat ccgtcgcgcc ctgtacataa
     2101 ccagcaagct ctcagatgcc aactgctgcc tggacgccat ctgctactac tacatggcca
     2161 aggagttcca ggaggcgtct gcactggccg tggctcccag tgctaaggcc cacaaaagcc
     2221 aggactctct gtgcgtgacc ctcgccttgc caactttctt gtacaaagtg gttgatatcg
     2281 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtaccg
     2341 gttaagtcga caatcaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     2401 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2461 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2521 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2581 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2641 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2701 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2761 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2821 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2881 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2941 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     3001 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     3061 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     3121 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3181 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3241 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     3301 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     3361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     3481 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3541 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3601 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     3661 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     3721 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3781 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3841 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3901 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3961 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4021 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4081 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4141 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4201 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4261 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     4321 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4381 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4441 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4501 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4561 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4621 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4681 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4741 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4801 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4861 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4921 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4981 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5041 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5101 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5161 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5221 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5281 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5341 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5401 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5461 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5521 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5581 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5641 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5701 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5761 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5821 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5881 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5941 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6001 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6061 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6121 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6181 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6241 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6301 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6361 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6421 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     6481 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     6541 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     6601 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     6661 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     6721 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     6781 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6841 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6901 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     6961 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     7021 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     7081 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     7141 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     7201 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     7261 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     7321 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     7381 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     7441 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     7501 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     7561 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     7621 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     7681 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     7741 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     7801 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     7861 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     7921 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     7981 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     8041 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     8101 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     8161 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     8221 ggggacccat gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     8281 cccagcaggc agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc
     8341 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8461 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac
     8521 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     8581 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     8641 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     8701 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct
     8761 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     8821 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     8881 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     8941 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     9001 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     9061 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     9121 agcccggtgc cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat
     9181 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     9241 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     9301 gtttattaca gggacagcag agatccactt tggcgcc
//
