LOCUS       sequence-180247-       10084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HNF1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: HNF1B_pLX307
FEATURES             Location/Qualifiers
     source          1..10084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2995..3019)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3027..3068
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3119..3707
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3172..3192)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3590..3601)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3779..4012
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4090..4211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4127..4146)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4181..4200
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4251..4386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4406..4425)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4407..4425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4435..4452)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4435..4451)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4444..4466)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4593..5048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4680..4699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4890..4911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5074..5178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5179..5247,5248..6039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5397..5416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6210..6798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6699..6718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6952..6969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7086..7107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7122..7127,7128..7145,7146..7152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7160..7176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7165..7187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7184..7200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7184..7200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7219..7239
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7221..7239
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7267..7493
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7494..7674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7721..7846
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8339..8572
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8757..8801
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9032..9242
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9054..9250
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9101..9236
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9282..9884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9282..9301)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9778..9798
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9927..10044
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggtgtccaa gctcacgtcg ctccagcaag aactcctgag cgccctgctg agctccgggg
     1381 tcaccaagga ggtgctggtt caggccttgg aggagttgct gccatccccg aacttcgggg
     1441 tgaagctgga gacgctgccc ctgtcccctg gcagcggggc cgagcccgac accaagccgg
     1501 tcttccatac tctcaccaac ggccacgcca agggccgctt gtccggcgac gagggctccg
     1561 aggacggcga cgactatgac acacctccca tcctcaagga gctgcaggcg ctcaacaccg
     1621 aggaggcggc ggagcagcgg gcggaggtgg accggatgct cagtgaggac ccttggaggg
     1681 ctgctaaaat gatcaagggt tacatgcagc aacacaacat cccccagagg gaggtggtcg
     1741 atgtcaccgg cctgaaccag tcgcacctct cccagcatct caacaagggc acccctatga
     1801 agacccagaa gcgtgccgct ctgtacacct ggtacgtcag aaagcaacga gagatcctcc
     1861 gacaattcaa ccagacagtc cagagttctg gaaatatgac agacaaaagc agtcaggatc
     1921 agctgctgtt tctctttcca gagttcagtc aacagagcca tgggcctggg cagtccgatg
     1981 atgcctgctc tgagcccacc aacaagaaga tgcgccgcaa ccggttcaaa tgggggcccg
     2041 cgtcccagca aatcttgtac caggcctacg atcggcaaaa gaaccccagc aaggaagaga
     2101 gagaggcctt agtggaggaa tgcaacaggg cagaatgttt gcagcgaggg gtgtccccct
     2161 ccaaagccca cggcctgggc tccaacttgg tcactgaggt ccgtgtctac aactggtttg
     2221 caaaccgcag gaaggaggag gcattccggc aaaagctggc catggacgcc tatagctcca
     2281 accagactca cagcctgaac cctctgctct cccacggctc cccccaccac cagcccagct
     2341 cctctcctcc aaacaagctg tcaggagtgc gctacagcca gcagggaaac aatgagatca
     2401 cttcctcctc aacaatcagt caccatggca acagcgccat ggtgaccagc cagtcggttt
     2461 tacagcaagt ctccccagcc agcctggacc caggccacaa tctcctctca cctgatggta
     2521 aaatgatctc agtctcagga ggaggtttgc ccccagtcag caccttgacg aatatccaca
     2581 gcctctccca ccataatccc cagcaatctc aaaacctcat catgacaccc ctctctggag
     2641 tcatggcaat tgcacaaagc ctcaacacct cccaagcaca gagtgtccct gtcatcaaca
     2701 gtgtggccgg cagcctggca gccctgcagc ccgtccagtt ctcccagcag ctgcacagcc
     2761 ctcaccagca gcccctcatg cagcagagcc caggcagcca catggcccag cagcccttca
     2821 tggcagctgt gactcagctg cagaactcac acatgtacgc acacaagcag gaaccccccc
     2881 agtattccca cacctcccgg tttccatctg caatggtggt cacagatacc agcagcatca
     2941 gtacactcac caacatgtct tcaagtaaac agtgtcctct acaagcctgg gaacccccaa
     3001 ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg
     3061 attctacgta gtaatgaact agtaccggtt aagtcgacaa tcaacgcgtt aagtcgacaa
     3121 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3181 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3241 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3301 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3361 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3421 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3481 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3541 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3601 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3661 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     3721 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     3781 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     3841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     4021 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     4081 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4141 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4201 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     4261 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4321 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4381 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4441 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4501 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4561 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     4621 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4681 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4741 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4801 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4861 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4921 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4981 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5041 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5101 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5161 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5221 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5401 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5881 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6901 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6961 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7021 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7081 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7141 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7201 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7261 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7321 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7381 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7441 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7501 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7561 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7621 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7681 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7741 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7801 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     7861 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     7921 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     7981 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8041 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8101 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8161 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8221 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8281 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8341 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8401 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8461 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8521 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8581 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8641 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8701 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8761 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8821 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8881 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     8941 cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc
     9001 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct
     9061 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9121 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9181 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9241 cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca
     9301 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     9361 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     9421 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     9481 cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     9541 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     9601 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     9661 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     9721 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     9781 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     9841 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg
     9901 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     9961 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    10021 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
    10081 cgcc
//
