LOCUS       sequence-180248-       10348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HSPA8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: HSPA8_pLX307
FEATURES             Location/Qualifiers
     source          1..10348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3259..3283)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3291..3332
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3383..3971
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3436..3456)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3854..3865)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4043..4276
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4354..4475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4391..4410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4445..4464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4515..4650
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4670..4689)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4671..4689)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4699..4716)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4699..4715)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4708..4730)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4857..5312
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4944..4963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5154..5175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5338..5442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5443..5511,5512..6303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5661..5680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6474..7062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6963..6982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7216..7233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7350..7371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7386..7391,7392..7409,7410..7416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7424..7440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7429..7451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7448..7464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7448..7464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7483..7503
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7485..7503
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7531..7757
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7758..7938
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7985..8110
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8603..8836
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9021..9065
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9296..9506
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9318..9514
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9365..9500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9546..10148
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9546..9565)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10042..10062
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10191..10308
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgtccaaggg acctgcagtt ggtattgatc ttggcaccac ctactcttgt gtgggtgttt
     1381 tccagcacgg aaaagtcgag ataattgcca atgatcaggg aaaccgaacc actccaagct
     1441 atgtcgcctt tacggacact gaacggttga tcggtgatgc cgcaaagaat caagttgcaa
     1501 tgaaccccac caacacagtt tttgatgcca aacgtctgat tggacgcaga tttgatgatg
     1561 ctgttgtcca gtctgatatg aaacattggc cctttatggt ggtgaatgat gctggcaggc
     1621 ccaaggtcca agtagaatac aagggagaga ccaaaagctt ctatccagag gaggtgtctt
     1681 ctatggttct gacaaagatg aaggaaattg cagaagccta ccttgggaag actgttacca
     1741 atgctgtggt cacagtgcca gcttacttta atgactctca gcgtcaggct accaaagatg
     1801 ctggaactat tgctggtctc aatgtactta gaattattaa tgagccaact gctgctgcta
     1861 ttgcttacgg cttagacaaa aaggttggag cagaaagaaa cgtgctcatc tttgacctgg
     1921 gaggtggcac ttttgatgtg tcaatcctca ctattgagga tggaatcttt gaggtcaagt
     1981 ctacagctgg agacacccac ttgggtggag aagattttga caaccgaatg gtcaaccatt
     2041 ttattgctga gtttaagcgc aagcataaga aggacatcag tgagaacaag agagctgtaa
     2101 gacgcctccg tactgcttgt gaacgtgcta agcgtaccct ctcttccagc acccaggcca
     2161 gtattgagat cgattctctc tatgaaggaa tcgacttcta tacctccatt acccgtgccc
     2221 gatttgaaga actgaatgct gacctgttcc gtggcaccct ggacccagta gagaaagccc
     2281 ttcgagatgc caaactagac aagtcacaga ttcatgatat tgtcctggtt ggtggttcta
     2341 ctcgtatccc caagattcag aagcttctcc aagacttctt caatggaaaa gaactgaata
     2401 agagcatcaa ccctgatgaa gctgttgctt atggtgcagc tgtccaggca gccatcttgt
     2461 ctggagacaa gtctgagaat gttcaagatt tgctgctctt ggatgtcact cctctttccc
     2521 ttggtattga aactgctggt ggagtcatga ctgtcctcat caagcgtaat accaccattc
     2581 ctaccaagca gacacagacc ttcactacct attctgacaa ccagcctggt gtgcttattc
     2641 aggtttatga aggcgagcgt gccatgacaa aggataacaa cctgcttggc aagtttgaac
     2701 tcacaggcat acctcctgca ccccgaggtg ttcctcagat tgaagtcact tttgacattg
     2761 atgccaatgg tatactcaat gtctctgctg tggacaagag tacgggaaaa gagaacaaga
     2821 ttactatcac taatgacaag ggccgtttga gcaaggaaga cattgaacgt atggtccagg
     2881 aagctgagaa gtacaaagct gaagatgaga agcagaggga caaggtgtca tccaagaatt
     2941 cacttgagtc ctatgccttc aacatgaaag caactgttga agatgagaaa cttcaaggca
     3001 agattaacga tgaggacaaa cagaagattc tggacaagtg taatgaaatt atcaactggc
     3061 ttgataagaa tcagactgct gagaaggaag aatttgaaca tcaacagaaa gagctggaga
     3121 aagtttgcaa ccccatcatc accaagctgt accagagtgc aggaggcatg ccaggaggaa
     3181 tgcctggggg atttcctggt ggtggagctc ctccctctgg tggtgcttcc tcagggccca
     3241 ccattgaaga ggttgattac ccaactttct tgtacaaagt ggttgatatc ggtaagccta
     3301 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtacc ggttaagtcg
     3361 acaatcaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     3421 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3481 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3541 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3601 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3661 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3721 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3781 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3841 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3901 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3961 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4021 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     4081 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     4141 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4201 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4261 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     4321 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     4381 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4441 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     4501 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4561 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4621 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     4681 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4741 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4801 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     4861 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4921 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4981 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5041 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5101 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5161 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5221 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5281 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     5341 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5401 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5461 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5521 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5581 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5641 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5701 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5761 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5821 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5881 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5941 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6001 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6061 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6121 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6181 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6241 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6301 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6361 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6421 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6481 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6541 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6601 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6661 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6721 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6781 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6841 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6901 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6961 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7021 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7081 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7141 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7201 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7261 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7321 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7381 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7441 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     7501 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     7561 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     7621 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     7681 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     7741 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7801 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7861 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7921 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     7981 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     8041 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     8101 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     8161 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     8221 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     8281 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     8341 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     8401 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     8461 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     8521 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     8581 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     8641 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     8701 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     8761 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     8821 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     8881 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     8941 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     9001 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     9061 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     9121 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     9181 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca
     9241 tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9301 cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc
     9361 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9421 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9481 aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc
     9541 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     9601 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     9661 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     9721 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc
     9781 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     9841 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     9901 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     9961 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
    10021 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
    10081 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
    10141 ccagttaacg aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
    10201 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
    10261 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
    10321 agggacagca gagatccact ttggcgcc
//
