LOCUS       sequence-180249-       12508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IGF1R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12508)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 12508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: IGF1R_pLX307
FEATURES             Location/Qualifiers
     source          1..12508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5419..5443)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5451..5492
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5543..6131
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5596..5616)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6014..6025)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6203..6436
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6514..6635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6551..6570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6605..6624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6675..6810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6830..6849)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6831..6849)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6859..6876)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6859..6875)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6868..6890)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7017..7472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7104..7123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7314..7335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7498..7602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7603..7671,7672..8463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7821..7840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8634..9222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9123..9142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9376..9393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9510..9531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9546..9551,9552..9569,9570..9576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9584..9600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9589..9611
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9608..9624
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9608..9624
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9643..9663
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9645..9663
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9691..9917
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9918..10098
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10145..10270
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10763..10996
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11181..11225
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11456..11666
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11478..11674
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11525..11660
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11706..12308
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11706..11725)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12202..12222
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12351..12468
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgaagtctgg ctccggagga gggtccccga cctcgctgtg ggggctcctg tttctctccg
     1381 ccgcgctctc gctctggccg acgagtggag aaatctgcgg gccaggcatc gacatccgca
     1441 acgactatca gcagctgaag cgcctggaga actgcacggt gatcgagggc tacctccaca
     1501 tcctgctcat ctccaaggcc gaggactacc gcagctaccg cttccccaag ctcacggtca
     1561 ttaccgagta cttgctgctg ttccgagtgg ctggcctcga gagcctcgga gacctcttcc
     1621 ccaacctcac ggtcatccgc ggctggaaac tcttctacaa ctacgccctg gtcatcttcg
     1681 agatgaccaa tctcaaggat attgggcttt acaacctgag gaacattact cggggggcca
     1741 tcaggattga gaaaaatgct gacctctgtt acctctccac tgtggactgg tccctgatcc
     1801 tggatgcggt gtccaataac tacattgtgg ggaataagcc cccaaaggaa tgtggggacc
     1861 tgtgtccagg gaccatggag gagaagccga tgtgtgagaa gaccaccatc aacaatgagt
     1921 acaactaccg ctgctggacc acaaaccgct gccagaaaat gtgcccaagc acgtgtggga
     1981 agcgggcgtg caccgagaac aatgagtgct gccaccccga gtgcctgggc agctgcagcg
     2041 cgcctgacaa cgacacggcc tgtgtagctt gccgccacta ctactatgcc ggtgtctgtg
     2101 tgcctgcctg cccgcccaac acctacaggt ttgagggctg gcgctgtgtg gaccgtgact
     2161 tctgcgccaa catcctcagc gccgagagca gcgactccga ggggtttgtg atccacgacg
     2221 gcgagtgcat gcaggagtgc ccctcgggct tcatccgcaa cggcagccag agcatgtact
     2281 gcatcccttg tgaaggtcct tgcccgaagg tctgtgagga agaaaagaaa acaaagacca
     2341 ttgattctgt tacttctgct cagatgctcc aaggatgcac catcttcaag ggcaatttgc
     2401 tcattaacat ccgacggggg aataacattg cttcagagct ggagaacttc atggggctca
     2461 tcgaggtggt gacgggctac gtgaagatcc gccattctca tgccttggtc tccttgtcct
     2521 tcctaaaaaa ccttcgcctc atcctaggag aggagcagct agaagggaat tactccttct
     2581 acgtcctcga caaccagaac ttgcagcaac tgtgggactg ggaccaccgc aacctgacca
     2641 tcaaagcagg gaaaatgtac tttgctttca atcccaaatt atgtgtttcc gaaatttacc
     2701 gcatggagga agtgacgggg actaaagggc gccaaagcaa aggggacata aacaccagga
     2761 acaacgggga gagagcctcc tgtgaaagtg acgtcctgca tttcacctcc accaccacgt
     2821 cgaagaatcg catcatcata acctggcacc ggtaccggcc ccctgactac agggatctca
     2881 tcagcttcac cgtttactac aaggaagcac cctttaagaa tgtcacagag tatgatgggc
     2941 aggatgcctg cggctccaac agctggaaca tggtggacgt ggacctcccg cccaacaagg
     3001 acgtggagcc cggcatctta ctacatgggc tgaagccctg gactcagtac gccgtttacg
     3061 tcaaggctgt gaccctcacc atggtggaga acgaccatat ccgtggggcc aagagtgaga
     3121 tcttgtacat tcgcaccaat gcttcagttc cttccattcc cttggacgtt ctttcagcat
     3181 cgaactcctc ttctcagtta atcgtgaagt ggaaccctcc ctctctgccc aacggcaacc
     3241 tgagttacta cattgtgcgc tggcagcggc agcctcagga cggctacctt taccggcaca
     3301 attactgctc caaagacaaa atccccatca ggaagtatgc cgacggcacc atcgacattg
     3361 aggaggtcac agagaacccc aagactgagg tgtgtggtgg ggagaaaggg ccttgctgcg
     3421 cctgccccaa aactgaagcc gagaagcagg ccgagaagga ggaggctgaa taccgcaaag
     3481 tctttgagaa tttcctgcac aactccatct tcgtgcccag acctgaaagg aagcggagag
     3541 atgtcatgca agtggccaac accaccatgt ccagccgaag caggaacacc acggccgcag
     3601 acacctacaa catcaccgac ccggaagagc tggagacaga gtaccctttc tttgagagca
     3661 gagtggataa caaggagaga actgtcattt ctaaccttcg gcctttcaca ttgtaccgca
     3721 tcgatatcca cagctgcaac cacgaggctg agaagctggg ctgcagcgcc tccaacttcg
     3781 tctttgcaag gactatgccc gcagaaggag cagatgacat tcctgggcca gtgacctggg
     3841 agccaaggcc tgaaaactcc atctttttaa agtggccgga acctgagaat cccaatggat
     3901 tgattctaat gtatgaaata aaatacggat cacaagttga ggatcagcga gaatgtgtgt
     3961 ccagacagga atacaggaag tatggagggg ccaagctaaa ccggctaaac ccggggaact
     4021 acacagcccg gattcaggcc acatctctct ctgggaatgg gtcgtggaca gatcctgtgt
     4081 tcttctatgt ccaggccaaa acaggatatg aaaacttcat ccatctgatc atcgctctgc
     4141 ccgtcgctgt cctgttgatc gtgggagggt tggtgattat gctgtacgtc ttccatagaa
     4201 agagaaataa cagcaggctg gggaatggag tgctgtatgc ctctgtgaac ccggagtact
     4261 tcagcgctgc tgatgtgtac gttcctgatg agtgggaggt ggctcgggag aagatcacca
     4321 tgagccggga acttgggcag gggtcgtttg ggatggtcta tgaaggagtt gccaagggtg
     4381 tggtgaaaga tgaacctgaa accagagtgg ccattaaaac agtgaacgag gccgcaagca
     4441 tgcgtgagag gattgagttt ctcaacgaag cttctgtgat gaaggagttc aattgtcacc
     4501 atgtggtgcg attgctgggt gtggtgtccc aaggccagcc aacactggtc atcatggaac
     4561 tgatgacacg gggcgatctc aaaagttatc tccggtctct gaggccagaa atggagaata
     4621 atccagtcct agcacctcca agcctgagca agatgattca gatggccgga gagattgcag
     4681 acggcatggc atacctcaac gccaataagt tcgtccacag agaccttgct gcccggaatt
     4741 gcatggtagc cgaagatttc acagtcaaaa tcggagattt tggtatgacg cgagatatct
     4801 atgagacaga ctattaccgg aaaggaggga aagggctgct gcccgtgcgc tggatgtctc
     4861 ctgagtccct caaggatgga gtcttcacca cttactcgga cgtctggtcc ttcggggtcg
     4921 tcctctggga gatcgccaca ctggccgagc agccctacca gggcttgtcc aacgagcaag
     4981 tccttcgctt cgtcatggag ggcggccttc tggacaagcc agacaactgt cctgacatgc
     5041 tgtttgaact gatgcgcatg tgctggcagt ataaccccaa gatgaggcct tccttcctgg
     5101 agatcatcag cagcatcaaa gaggagatgg agcctggctt ccgggaggtc tccttctact
     5161 acagcgagga gaacaagctg cccgagccgg aggagctgga cctggagcca gagaacatgg
     5221 agagcgtccc cctggacccc tcggcctcct cgtcctccct gccactgccc gacagacact
     5281 caggacacaa ggccgagaac ggccccggcc ctggggtgct ggtcctccgc gccagcttcg
     5341 acgagagaca gccttacgcc cacatgaacg ggggccgcaa gaacgagcgg gccttgccgc
     5401 tgccccagtc ttcgacctgc ccaactttct tgtacaaagt ggttgatatc ggtaagccta
     5461 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtacc ggttaagtcg
     5521 acaatcaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     5581 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5641 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     5701 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     5761 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5821 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5881 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     5941 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6001 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6061 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6121 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6181 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6241 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6301 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6361 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6421 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     6481 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6661 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6781 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     6841 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6901 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     7021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7381 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7441 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     7501 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7561 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7621 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7681 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7741 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7801 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7861 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7921 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7981 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8041 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8101 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8161 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8221 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8281 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8341 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8401 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8461 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8521 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8581 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8641 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8701 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8761 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8821 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8881 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8941 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9001 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9061 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     9481 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     9541 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     9601 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     9661 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     9721 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     9781 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     9841 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     9901 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9961 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    10021 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    10081 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
    10141 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
    10201 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
    10261 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
    10321 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
    10381 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
    10441 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
    10501 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
    10561 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
    10621 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    10681 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    10741 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    10801 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    10861 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    10921 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    10981 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    11041 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    11101 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    11161 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    11221 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    11281 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    11341 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca
    11401 tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    11461 cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc
    11521 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    11581 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    11641 aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc
    11701 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
    11761 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
    11821 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
    11881 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc
    11941 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
    12001 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
    12061 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
    12121 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
    12181 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
    12241 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
    12301 ccagttaacg aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
    12361 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
    12421 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
    12481 agggacagca gagatccact ttggcgcc
//
