LOCUS       sequence-180250-        9562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of APOBEC3G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: APOBEC3G_pLX307
FEATURES             Location/Qualifiers
     source          1..9562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2473..2497)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2505..2546
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2597..3185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2650..2670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3068..3079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3257..3490
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3568..3689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3605..3624)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3659..3678
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3729..3864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3884..3903)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3885..3903)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3913..3930)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3913..3929)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3922..3944)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4071..4526
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4158..4177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4368..4389
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4552..4656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4657..4725,4726..5517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4875..4894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5688..6276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6177..6196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6430..6447
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6564..6585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6600..6605,6606..6623,6624..6630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6638..6654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6643..6665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6662..6678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6662..6678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6697..6717
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6699..6717
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6745..6971
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6972..7152
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7199..7324
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7817..8050
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8235..8279
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8510..8720
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8532..8728
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8579..8714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8760..9362
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8760..8779)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9256..9276
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9405..9522
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgaagcctca cttcagaaac acagtggagc gaatgtatcg agacacattc tcctacaact
     1381 tttataatag acccatcctt tctcgtcgga ataccgtctg gctgtgctac gaagtgaaaa
     1441 caaagggtcc ctcaaggccc cctttggacg caaagatctt tcgaggccag gtgtattccg
     1501 aacttaagta ccacccagag atgagattct tccactggtt cagcaagtgg aggaagctgc
     1561 atcgtgacca ggagtatgag gtcacctggt acatatcctg gagcccctgc acaaagtgta
     1621 caagggatat ggccacgttc ctggccgagg acccgaaggt taccctgacc atctttgttg
     1681 cccgcctcta ctacttctgg gacccagatt accaggaggc gcttcgcagc ctgtgtcaga
     1741 aaagagacgg tccgcgtgcc accatgaaga tcatgaatta tgacgaattt cagcactgtt
     1801 ggagcaagtt cgtgtacagc caaagagagc tatttgagcc ttggaataat ctgcctaaat
     1861 attatatatt actgcacatc atgctggggg agattctcag acactcgatg gatccaccca
     1921 cattcacttt caactttaac aatgaacctt gggtcagagg acggcatgag acttacctgt
     1981 gttatgaggt ggagcgcatg cacaatgaca cctgggtcct gctgaaccag cgcaggggct
     2041 ttctatgcaa ccaggctcca cataaacacg gtttccttga aggccgccat gcagagctgt
     2101 gcttcctgga cgtgattccc ttttggaagc tggacctgga ccaggactac agggttacct
     2161 gcttcacctc ctggagcccc tgcttcagct gtgcccagga aatggctaaa ttcatttcaa
     2221 aaaacaaaca cgtgagcctg tgcatcttca ctgcccgcat ctatgatgat caaggaagat
     2281 gtcaggaggg gctgcgcacc ctggccgagg ctggggccaa aatttcaata atgacataca
     2341 gtgaatttaa gcactgctgg gacacctttg tggaccacca gggatgtccc ttccagccct
     2401 gggatggact agatgagcac agccaagacc tgagtgggag gctgcgggcc attctccaga
     2461 atcaggaaaa ctgcccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta
     2521 accctctcct cggtctcgat tctacgtagt aatgaactag taccggttaa gtcgacaatc
     2581 aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2641 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2701 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2761 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2821 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2881 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2941 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3001 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3061 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3121 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3181 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3241 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     3301 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3361 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3421 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3481 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     3541 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     3601 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3661 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     3721 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3781 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3841 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     3901 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3961 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4021 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     4081 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4141 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4201 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4261 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4321 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4381 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4441 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4501 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     4561 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4621 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4681 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4741 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4801 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4861 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4921 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4981 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5041 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5101 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5161 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5221 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5281 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5341 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5401 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5461 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5521 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5581 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5641 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5701 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5761 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5821 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5881 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5941 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6001 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6061 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6121 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6181 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6241 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6301 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6361 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6421 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6481 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6541 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     6601 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     6661 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     6721 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     6781 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     6841 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     6901 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     6961 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7021 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7081 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7141 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     7201 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     7261 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     7321 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     7381 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     7441 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     7501 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     7561 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     7621 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     7681 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     7741 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     7801 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     7861 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     7921 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     7981 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     8041 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     8101 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     8161 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     8221 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     8281 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     8341 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     8401 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt
     8461 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8521 tatgcaaagc atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8581 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8641 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8701 cttttttgga ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca
     8761 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     8821 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     8881 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     8941 tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga
     9001 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     9061 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     9121 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     9181 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     9241 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     9301 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt
     9361 aacgaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     9421 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9481 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9541 agcagagatc cactttggcg cc
//
