LOCUS       sequence-180259-        9319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MTCH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: MTCH2_pLX307
FEATURES             Location/Qualifiers
     source          1..9319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2230..2254)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2262..2303
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2354..2942
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2407..2427)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2825..2836)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3014..3247
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3325..3446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3362..3381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3416..3435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3486..3621
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3641..3660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3642..3660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3670..3687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3670..3686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3679..3701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3828..4283
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3915..3934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4125..4146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4309..4413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4414..4482,4483..5274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4632..4651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5445..6033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5934..5953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6187..6204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6321..6342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6357..6362,6363..6380,6381..6387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6395..6411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6400..6422
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6419..6435
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6419..6435
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6454..6474
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6456..6474
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6502..6728
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6729..6909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6956..7081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7574..7807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7992..8036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8267..8477
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8289..8485
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8336..8471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8517..9119
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8517..8536)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9013..9033
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9162..9279
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggcggacgc ggccagtcag gtgctcctgg gctccggtct caccatcctg tcccagccgc
     1381 tcatgtacgt gaaagtgctc atccaggtgg gatatgagcc tcttcctcca acaataggac
     1441 gaaatatttt tgggcggcaa gtgtgtcagc ttcctggtct ctttagttat gctcagcaca
     1501 ttgccagtat cgatgggagg cgcgggttgt tcacaggctt aactccaaga ctgtgttcgg
     1561 gagtccttgg aactgtggtc catggtaaag ttttacagca ttaccaggag agtgacaagg
     1621 gtgaggagtt aggacctgga aatgtacaga aagaagtctc atcttccttt gaccacgtta
     1681 tcaaggagac aactcgagag atgatcgctc gttctgctgc taccctcatc acacatccct
     1741 tccatgtgat cactctgaga tctatggtac agttcattgg cagagaatcc aagtactgtg
     1801 gactttgtga ttccataata accatctatc gggaagaggg cattctagga tttttcgcgg
     1861 gtcttgttcc tcgccttcta ggtgacatcc tttctttgtg gctgtgtaac tcactggcct
     1921 acctcgtcaa tacctatgca ctggacagtg gggtttctac catgaatgaa atgaagagtt
     1981 attctcaagc tgtcacagga ttttttgcga gtatgttgac ctatcccttt gtgcttgtct
     2041 ccaatcttat ggctgtcaac aactgtggtc ttgctggtgg atgccctcct tactccccaa
     2101 tatatacgtc ttggatagac tgttggtgca tgctacaaaa agaggggaat atgagccgag
     2161 gaaatagctt atttttccgg aaggtcccct ttgggaagac ttattgttgt gacctgaaaa
     2221 tgttaatttg cccaactttc ttgtacaaag tggttgatat cggtaagcct atccctaacc
     2281 ctctcctcgg tctcgattct acgtagtaat gaactagtac cggttaagtc gacaatcaac
     2341 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2401 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2461 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2521 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2581 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2641 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2701 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2761 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2821 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2881 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2941 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     3001 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     3061 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3121 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3181 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3241 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     3301 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     3361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3421 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     3481 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3541 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     3601 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     3661 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     3721 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     3781 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     3841 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3901 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3961 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4021 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4081 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4141 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4201 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4261 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     4321 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4381 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4441 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4501 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4561 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4621 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4681 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4741 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4801 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4861 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4921 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4981 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5041 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5101 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5161 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5221 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5281 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5341 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5581 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6001 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6061 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6121 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6181 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6241 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6301 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     6361 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6421 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     6481 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     6541 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     6601 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     6661 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     6721 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6781 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6841 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6901 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     6961 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     7021 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     7081 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     7141 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     7201 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     7261 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     7321 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     7381 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     7441 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     7501 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     7561 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     7621 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     7681 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     7741 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     7801 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     7861 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     7921 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     7981 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     8041 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     8101 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     8161 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca
     8221 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8281 gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8341 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8401 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8461 ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga
     8521 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     8581 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     8641 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     8701 gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg
     8761 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     8821 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     8881 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     8941 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     9001 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     9061 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac
     9121 gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     9181 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     9241 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     9301 agagatccac tttggcgcc
//
