LOCUS       sequence-180262-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of OXSM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: OXSM_pLX307
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2479..2490
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(2698..2722)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2730..2771
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2822..3410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2875..2895)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3293..3304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3482..3715
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3793..3914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3830..3849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3884..3903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3954..4089
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4109..4128)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4110..4128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4138..4155)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4138..4154)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4147..4169)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4296..4751
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4383..4402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4593..4614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4777..4881
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4882..4950,4951..5742)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5100..5119)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5913..6501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6402..6421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6655..6672
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6789..6810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6825..6830,6831..6848,6849..6855)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6863..6879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6868..6890
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6887..6903
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6887..6903
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6922..6942
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6924..6942
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6970..7196
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7197..7377
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7424..7549
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8042..8275
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8460..8504
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8735..8945
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8757..8953
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8804..8939
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8985..9587
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8985..9004)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9481..9501
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9630..9747
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgtccaactg cctgcaaaat ttcctgaaaa ttacaagcac tcgtcttcta tgttcaagat
     1381 tatgccaaca gttaagaagt aaaaggaagt ttttcggaac tgtgccaata tccagattgc
     1441 ataggcgagt tgtcattaca ggcattggct tagtgactcc tcttggtgtt ggaactcacc
     1501 tggtttggga tcgtcttatc ggaggagaga gtggaattgt ttcactggtt ggtgaagagt
     1561 ataagagtat cccttgcagt gttgctgctt atgtgccaag aggtagtgat gaaggtcagt
     1621 tcaatgaaca aaactttgtg tccaaatcag atatcaagtc catgtcttct cccaccatca
     1681 tggccattgg ggctgcagaa ttagccatga aggattctgg ctggcatcct cagtcagaag
     1741 ctgatcaagt ggctactggt gttgcaattg gcatgggaat gattcctctt gaagttgttt
     1801 ctgaaactgc tttgaatttt cagacaaaag gttacaataa agttagccca ttttttgtcc
     1861 ctaagattct ggtcaatatg gcagcaggcc aggtcagcat tcgatataaa ctcaagggcc
     1921 caaatcatgc agtatccaca gcctgtacca caggagctca tgctgtggga gactcattta
     1981 gatttatagc ccatggtgat gctgatgtga tggtggctgg aggtacagat tcttgtatta
     2041 gccctttatc tcttgctggg ttttccagag cccgggctct gagcacaaac tcagatccca
     2101 agttggcatg tcgaccattt catccaaaga gagatggttt tgtaatggga gaaggtgcag
     2161 ctgtgctggt gctggaagaa tatgaacatg ctgttcaaag aagagcccgg atctatgcag
     2221 aagttttggg ctatggactc tcaggtgatg ctggtcacat aactgcccct gatcctgaag
     2281 gagaaggtgc cttaaggtgt atggctgctg ctttaaaaga tgcaggtgtg cagcctgagg
     2341 agatatccta tatcaatgca catgctactt ccacaccatt gggagatgct gctgaaaaca
     2401 aagctatcaa acatctcttc aaagaccatg catatgccct tgcagtttcc tcaactaagg
     2461 gagcaacagg acatctgctg ggagctgcag gggcagtcga ggcagctttt accacattag
     2521 cttgttatta tcaaaaacta ccacctactt taaacctgga ttgttcggaa ccagaatttg
     2581 atctcaacta tgttccacta aaggcacagg aatggaaaac tgagaaaaga tttattggcc
     2641 tcaccaattc ctttggtttt ggtggtacta atgcaacact ttgtattgct ggactgtacc
     2701 caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct ctcctcggtc
     2761 tcgattctac gtagtaatga actagtaccg gttaagtcga caatcaacgc gttaagtcga
     2821 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2881 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2941 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3001 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3061 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3121 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3181 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3241 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3301 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3361 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     3421 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3481 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     3541 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3601 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3661 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     3721 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     3781 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3841 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3901 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     3961 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4021 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4081 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     4141 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4201 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4261 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     4321 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4381 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4441 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4501 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4561 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4621 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4681 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4741 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4801 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4861 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4921 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4981 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5041 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5101 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5161 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5221 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5281 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5341 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5401 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5461 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5521 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5581 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5641 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5701 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5761 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5821 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5881 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5941 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6001 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6061 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6421 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6541 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6601 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6661 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6721 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6781 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6841 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6901 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     6961 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     7021 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     7081 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     7141 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     7201 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7261 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7321 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     7381 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     7441 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     7501 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     7561 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     7621 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     7681 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     7741 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     7801 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     7861 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     7921 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     7981 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     8041 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     8101 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     8161 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     8221 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     8281 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     8341 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     8401 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     8461 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     8521 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     8581 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     8641 tatcgtttca gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc
     8701 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg
     8761 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8821 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8881 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8941 aggcttttgc aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc
     9001 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     9061 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     9121 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     9181 tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     9241 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     9301 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     9361 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     9421 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     9481 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     9541 aaggaccgcg cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc
     9601 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     9661 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     9721 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     9781 tggcgcc
//
