LOCUS       sequence-180263-       11731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of POLD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: POLD1_pLX307
FEATURES             Location/Qualifiers
     source          1..11731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2115..2126)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(4642..4666)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4674..4715
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4766..5354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4819..4839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5237..5248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5426..5659
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5737..5858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5774..5793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5828..5847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5898..6033
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6053..6072)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6054..6072)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6082..6099)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6082..6098)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6091..6113)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6240..6695
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6327..6346)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6537..6558
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6721..6825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6826..6894,6895..7686)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7044..7063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7857..8445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8346..8365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8599..8616
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8733..8754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8769..8774,8775..8792,8793..8799)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8807..8823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8812..8834
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8831..8847
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8831..8847
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8866..8886
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8868..8886
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8914..9140
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9141..9321
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9368..9493
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9986..10219
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10404..10448
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10679..10889
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10701..10897
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10748..10883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10929..11531
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10929..10948)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11425..11445
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11574..11691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggatggcaa gcggcggcca ggcccagggc ccggggtgcc cccaaagcgg gcccgtgggg
     1381 gcctctggga tgatgatgat gcacctcggc catcccaatt cgaggaggac ctggcactga
     1441 tggaggagat ggaggcagaa cacaggctgc aggagcagga ggaggaggag ctgcagtcag
     1501 tcctggaggg ggttgcagac gggcaggtcc caccatcagc catagatcct cgctggcttc
     1561 ggcccacacc accagcgctg gacccccaga cagagcccct catcttccaa cagttggaga
     1621 ttgaccatta tgtgggccca gcgcagcctg tgcctggggg gcccccacca tcccgcggct
     1681 ccgtgcctgt gctccgcgcc ttcggggtca ccgatgaggg gttctctgtc tgctgccaca
     1741 tccacggctt cgctccctac ttctacaccc cagcgccccc tggtttcggg cccgagcaca
     1801 tgggtgacct gcaacgggag ctgaacttgg ccatcagccg ggacagtcgc ggggggaggg
     1861 agctgactgg gccggccgtg ctggctgtgg aactgtgctc ccgagagagc atgtttgggt
     1921 accacgggca cggcccctcc ccgttcctgc gcatcaccgt ggcgctgccg cgcctcgtgg
     1981 ccccggcccg ccgtctcctg gaacagggca tccgtgtggc aggcctgggc acgcccagct
     2041 tcgcgcccta cgaggccaac gtcgactttg agatccggtt catggtggac acggacatcg
     2101 tcggctgcaa ctggctggag ctcccagctg ggaaatacgc cctgaggctg aaggagaagg
     2161 ctacgcagtg ccagctggag gcggacgtgc tgtggtctga cgtggtcagt cacccaccgg
     2221 aagggccatg gcagcgcatt gcgcccttgc gcgtgctcag cttcgatatc gagtgcgccg
     2281 gccgcaaagg catcttccct gagcctgagc gggaccctgt catccagatc tgctcgctgg
     2341 gcctgcgctg gggggagccg gagcccttcc tacgcctggc gctcaccctg cggccctgtg
     2401 cccccatcct gggtgccaag gtgcagagct acgagaagga ggaggacctg ctgcaggcct
     2461 ggtccacctt catccgtatc atggaccccg acgtgatcac cggttacaac atccagaact
     2521 tcgaccttcc gtacctcatc tctcgggccc agaccctcaa ggtacaaaca ttccctttcc
     2581 tgggccgtgt ggccggcctt tgctccaaca tccgggactc ttcattccag tccaagcaga
     2641 cgggccggcg ggacaccaag gttgtcagca tggtgggccg cgtgcagatg gacatgctgc
     2701 aggtgctgct gcgggagtac aagctccgct cctacacgct caatgccgtg agcttccact
     2761 tcctgggcga gcagaaggag gacgtgcagc acagcatcat caccgacctg cagaatggga
     2821 acgaccagac ccgccgccgc ctggctgtgt actgcctgaa ggatgcctac ctgccactgc
     2881 ggctgctgga gcggctcatg gtgctggtga acgccgtgga gatggcgagg gtcactggcg
     2941 tgcccctcag ctacctgctc agtcgtggcc agcaggtcaa ggtcgtatcc cagctgttgc
     3001 ggcaggccat gcacgagggg ctgctgatgc ccgtggtgaa gtcagagggc ggcgaggact
     3061 acacgggagc cactgtcatc gagcccctca aagggtacta cgacgtcccc atcgccaccc
     3121 tggacttctc ctcgctgtac ccgtccatca tgatggccca caacctgtgt tacaccacgc
     3181 tccttcggcc cgggactgca cagaaactgg gcctgactga ggatcagttc atcaggaccc
     3241 ccaccgggga cgagtttgtg aagacctcag tgcggaaggg gctgctgccc cagatcctgg
     3301 agaacctgct cagtgcccgg aagagggcca aggccgagct ggccaaggag acagaccccc
     3361 tccggcgcca ggtcctggat ggacggcagc tggcgctgaa ggtgagcgcc aactccgtat
     3421 acggcttcac tggcgcccag gtgggcaagt tgccgtgcct ggagatctca cagagcgtca
     3481 cggggttcgg acgtcagatg atcgagaaaa ccaagcagct ggtggagtct aagtacacag
     3541 tggagaatgg ctacagcacc agtgccaagg tggtgtatgg tgacactgac tccgtcatgt
     3601 gccgattcgg cgtgtcctcg gtggctgagg cgatggccct ggggcgggag gccgcggact
     3661 gggtgtcagg tcacttcccg tcgcccatcc ggctggagtt tgagaaggtc tacttcccat
     3721 acctgcttat cagcaagaag cgctacgcgg gcctgctctt ctcctcccgg cccgacgccc
     3781 acgaccgcat ggactgcaag ggcctggagg ccgtgcgcag ggacaactgc cccctcgtgg
     3841 ccaacctggt cactgcctca ctgcgccgcc tgctcatcga ccgagaccct gagggcgcgg
     3901 tggctcacgc acaggacgtc atctcggacc tgctgtgcaa ccgcatcgat atctcccagc
     3961 tggtcatcac caaggagctg acccgcgcgg cctccgacta tgccggcaag caggcccacg
     4021 tggagctggc cgagaggatg aggaagcggg accccgggag tgcgcccagc ctgggcgacc
     4081 gcgtccccta cgtgatcatc agtgccgcca agggtgtggc cgcctacatg aagtcggagg
     4141 acccgctgtt cgtgctggag cacagcctgc ccattgacac gcagtactac ctggagcagc
     4201 agctggccaa gcccctcctg cgcatcttcg agcccatcct gggcgagggc cgtgccgagg
     4261 ctgtgctact gcggggggac cacacgcgct gcaagacggt gctcacgggc aaggtgggcg
     4321 gcctcctggc cttcgccaaa cgccgcaact gctgcattgg ctgccgcaca gtgctcagcc
     4381 accagggagc cgtgtgtgag ttctgccagc cccgggagtc tgagctgtat cagaaggagg
     4441 tatcccatct gaatgccctg gaggagcgct tctcgcgcct ctggacgcag tgccagcgct
     4501 gccagggcag cctgcacgag gacgtcatct gcaccagccg ggactgcccc atcttctaca
     4561 tgcgcaagaa ggtgcggaag gacctggaag accaggagca gctcctgcgg cgcttcggac
     4621 cccctggacc tgaggcctgg tgcccaactt tcttgtacaa agtggttgat atcggtaagc
     4681 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt accggttaag
     4741 tcgacaatca acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     4801 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4861 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4921 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4981 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5041 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5101 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5161 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5221 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5281 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5341 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     5401 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5461 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5521 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5581 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5641 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     5701 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     5761 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5821 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     5881 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5941 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6001 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     6061 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     6121 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     6181 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     6241 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6301 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6361 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6421 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6481 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6541 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6601 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6661 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     6721 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6781 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6841 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6901 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6961 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7021 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7081 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7141 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7201 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7261 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7321 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7381 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7441 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7501 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7561 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7621 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7681 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7741 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7801 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7861 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7921 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7981 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8041 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8101 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8161 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8221 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8281 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8341 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8401 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8461 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8521 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8581 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8641 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8701 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8761 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8821 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     8881 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     8941 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     9001 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     9061 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     9121 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     9181 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9241 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9301 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     9361 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     9421 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     9481 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     9541 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     9601 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     9661 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     9721 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     9781 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     9841 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9901 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9961 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    10021 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    10081 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    10141 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    10201 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    10261 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    10321 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    10381 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    10441 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    10501 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    10561 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    10621 ccatgcattg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    10681 aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac
    10741 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10801 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    10861 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg
    10921 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
    10981 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
    11041 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
    11101 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
    11161 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
    11221 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
    11281 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
    11341 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
    11401 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
    11461 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
    11521 gtgccagtta acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa
    11581 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    11641 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    11701 tacagggaca gcagagatcc actttggcgc c
//
