LOCUS       sequence-180270-        9013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RAB10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: RAB10_pLX307
FEATURES             Location/Qualifiers
     source          1..9013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1924..1948)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1956..1997
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2048..2636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2101..2121)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2519..2530)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2708..2941
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3019..3140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3056..3075)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3110..3129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3180..3315
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3335..3354)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3336..3354)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3364..3381)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3364..3380)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3373..3395)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3522..3977
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3609..3628)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3819..3840
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4003..4107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4108..4176,4177..4968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4326..4345)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5139..5727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5628..5647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5881..5898
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6015..6036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6051..6056,6057..6074,6075..6081)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6089..6105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6094..6116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6113..6129
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6113..6129
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6148..6168
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6150..6168
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6196..6422
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6423..6603
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6650..6775
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7268..7501
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7686..7730
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7961..8171
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7983..8179
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8030..8165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8211..8813
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8211..8230)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8707..8727
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8856..8973
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggcgaagaa gacgtacgac ctgcttttca agctgctcct gatcggggat tccggagtgg
     1381 ggaagacctg cgtccttttt cgtttttcgg atgatgcctt caatactacc tttatttcca
     1441 ccataggaat agacttcaag atcaaaacag ttgaattaca aggaaagaag atcaagctac
     1501 agatatggga tacagcaggc caggagcgat ttcacaccat cacaacctcc tactacagag
     1561 gcgcaatggg tatcatgcta gtatatgaca tcaccaatgg taaaagtttt gaaaacatca
     1621 gcaaatggct tagaaacata gatgagcatg ccaatgaaga tgtggaaaga atgttactag
     1681 gaaacaagtg tgatatggac gacaaaagag ttgtacctaa aggaaaagga gaacagattg
     1741 caagggagca tggtattagg ttttttgaga ctagtgcaaa agcaaatata aacatcgaaa
     1801 aggcgttcct cacgttagct gaagatatcc ttcgaaagac ccctgtaaaa gagcccaaca
     1861 gtgaaaatgt agatatcagc agtggaggag gcgtgacagg ctggaagagc aaatgctgca
     1921 gacccccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc
     1981 tcggtctcga ttctacgtag taatgaacta gtaccggtta agtcgacaat caacgcgtta
     2041 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2101 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2161 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2221 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2281 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2341 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2401 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2461 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2521 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2581 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     2641 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     2701 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     2761 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2821 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2881 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     2941 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     3001 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3061 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3121 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     3181 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3241 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3301 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     3361 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3421 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3481 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     3541 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3601 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3661 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3721 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3781 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3841 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3901 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3961 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4021 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4081 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4141 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4201 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4261 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4321 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4381 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4441 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4501 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4561 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4621 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4681 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4741 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4801 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4861 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4921 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4981 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5041 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5101 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5161 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5221 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5281 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5341 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5401 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5461 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5521 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5581 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5641 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5701 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5761 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5821 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5881 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5941 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6001 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6061 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6121 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     6181 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     6241 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     6301 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     6361 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     6421 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6481 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6541 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6601 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     6661 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     6721 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     6781 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     6841 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     6901 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     6961 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     7021 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7081 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     7141 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     7201 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     7261 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     7321 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     7381 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7441 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7501 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7561 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     7621 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     7681 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     7741 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     7801 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     7861 caccattatc gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa
     7921 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7981 catgcgtctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8041 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8101 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8161 aggcctaggc ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt
     8221 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     8281 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     8341 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8401 tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag
     8461 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     8521 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     8581 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     8641 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     8701 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     8761 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc
     8821 tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     8881 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     8941 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     9001 ccactttggc gcc
//
