LOCUS       sequence-180281-        9763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of TUBG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: TUBG1_pLX307
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2674..2698)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2706..2747
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2798..3386
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2851..2871)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3269..3280)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3458..3691
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3769..3890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3806..3825)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3860..3879
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3930..4065
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4085..4104)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4086..4104)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4114..4131)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4114..4130)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4123..4145)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4272..4727
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4359..4378)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4569..4590
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4753..4857
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4858..4926,4927..5718)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5076..5095)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5889..6477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6378..6397
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6631..6648
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6765..6786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6801..6806,6807..6824,6825..6831)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6839..6855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6844..6866
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6863..6879
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6863..6879
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6898..6918
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6900..6918
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6946..7172
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7173..7353
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7400..7525
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8018..8251
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8436..8480
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8711..8921
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8733..8929
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8780..8915
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8961..9563
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8961..8980)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9457..9477
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9606..9723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgccgaggga aatcatcacc ctacagttgg gccagtgcgg caatcagatt gggttcgagt
     1381 tctggaaaca gctgtgcgcc gagcatggta tcagccccga gggcatcgtg gaggagttcg
     1441 ccaccgaggg cactgaccgc aaggacgtct ttttctacca ggcagacgat gagcactaca
     1501 tcccccgggc cgtgctgctg gacttggaac cccgggtgat ccactccatc ctcaactccc
     1561 cctatgccaa gctctacaac ccagagaaca tctacctgtc ggaacatgga ggaggagctg
     1621 gcaacaactg ggccagcgga ttctcccagg gagaaaagat ccatgaggac atttttgaca
     1681 tcatagaccg ggaggcagat ggtagtgaca gtctagaggg ctttgtgctg tgtcactcca
     1741 ttgctggggg gacaggctct ggactgggtt cctacctctt agaacggctg aatgacaggt
     1801 atcctaagaa gctggtgcag acatactcag tgtttcccaa ccaggacgag atgagcgatg
     1861 tggtggtcca gccttacaat tcactcctca cactcaagag gctgacgcag aatgcagact
     1921 gtgtggtggt gctggacaac acagccctga accggattgc cacagaccgc ctgcacatcc
     1981 agaacccatc cttctcccag atcaaccagc tggtgtctac catcatgtca gccagcacca
     2041 ccaccctgcg ctaccctggc tacatgaaca atgacctcat cggcctcatc gcctcgctca
     2101 ttcccacccc acggctccac ttcctcatga ccggctacac ccctctcact acggaccagt
     2161 cagtggccag cgtgaggaag accacggtcc tggatgtcat gaggcggctg ctgcagccca
     2221 agaacgtgat ggtgtccaca ggccgagacc gccagaccaa ccactgctac atcgccatcc
     2281 tcaacatcat ccagggagag gtggacccca cccaggtcca caagagcttg cagaggatcc
     2341 gggaacgcaa gttggccaac ttcatcccgt ggggccccgc cagcatccag gtggccctgt
     2401 cgaggaagtc tccctacctg ccctcggccc accgggtcag cgggctcatg atggccaacc
     2461 acaccagcat ctcctcgctc ttcgagagaa cctgtcgcca gtatgacaag ctgcgtaagc
     2521 gggaggcctt cctggagcag ttccgcaagg aggacatgtt caaggacaac tttgatgaga
     2581 tggacacatc cagggagatt gtgcagcagc tcatcgatga gtaccatgcg gccacacggc
     2641 cagactacat ctcctggggc acccaggagc agtgcccaac tttcttgtac aaagtggttg
     2701 atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta
     2761 gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacctctgg attacaaaat
     2821 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2881 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     2941 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3001 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3061 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3121 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3181 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     3241 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3301 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3361 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     3421 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     3481 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     3541 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3601 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3661 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     3721 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     3781 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3841 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3901 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3961 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4021 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     4081 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     4141 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4201 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4261 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4321 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4381 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4441 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4501 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4561 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4621 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4681 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     4741 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4801 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4861 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4921 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4981 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5041 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5101 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5161 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5221 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5281 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5341 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5401 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5461 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5521 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5581 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5641 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5701 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5761 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5821 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5881 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5941 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6001 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6061 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6121 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6181 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6241 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6301 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6361 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6421 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6481 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6541 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6601 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6661 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     6721 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     6781 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     6841 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     6901 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     6961 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     7021 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     7081 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     7141 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     7201 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7261 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7321 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     7381 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     7441 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     7501 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     7561 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     7621 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     7681 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     7741 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     7801 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     7861 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     7921 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     7981 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     8041 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     8101 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     8161 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     8221 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     8281 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     8341 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     8401 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     8461 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     8521 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     8581 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     8641 ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8701 aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt
     8761 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8821 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8881 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag
     8941 aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     9001 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     9061 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     9121 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg
     9181 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     9241 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     9301 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     9361 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     9421 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     9481 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     9541 cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc
     9601 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     9661 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     9721 tttcgggttt attacaggga cagcagagat ccactttggc gcc
//
