LOCUS       sequence-180291-        6801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP5.2.1
FEATURES             Location/Qualifiers
     source          1..6801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1817..1834)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1823..2047
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2179..2767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2259..2278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3096..3143
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3168..3187
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3375..4163)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3486..3505)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4090..4109
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4198..4555)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4212..4347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4364..4555
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4512..4532
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4615..5406,5407..5475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5238..5257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5476..5580)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5607..6062
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5694..5713)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5904..5925
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6074..6453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6454..6657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6608..6628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6702..6721
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6702..6720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caccatgggt tgctctttct ctatcttcat ccttgcactt ctctcgtgct tgaccgtccc
       61 ggcctctgca gttccctacc gaaatgcctc tggggtctat cacgtcacca atgattgccc
      121 aaactcttcc atagtttacg aggctaatga cctgatctta cacgcacctg gttgtgtgcc
      181 ttgtgttagg acaggtaatg tcagtaggtg ctgggtccag atcaccccca cgctgtcagc
      241 cccgagcctt ggagcaatca cggcccctct tcggagggcc gtcgactacc ttgcgggagg
      301 ggctgccctt tgctccgcgt tatacgtcgg agacgcgtgt ggggcagtgt tcttggtagg
      361 ccaaatgttc acctataggc ctcgccagca tgctacggtg caggactgca actgttctat
      421 ctacagtggc cacgtcaccg gccaccggat ggcatgggac atgatgatga attggtcacc
      481 cacgacagcc ttgctgatgg cccagttgct acggatcccc caggtggtcg ttgacatcat
      541 cgccggcggc cactgggggg tcttgttcgc cgccgcatac tttgcgtcag cggctaactg
      601 ggctaaggtt gtgctggtct tgttcctgtt cgcaggggtt gatgccgaaa cccactcagt
      661 gggcggtgtt gtcgcccgcg acttgcgcag cctgaccagc attttcaccc ccgggccgcg
      721 gcagaacttg cagctcgtga acaccaacgg gagctggcac atcaacagaa ctgctcttaa
      781 ttgtcaagac agcctccaga ctgggtttat agctggcctc ctgtacttca acaagttcaa
      841 ctcctctggg tgtccagagc ggatggctag ctgcaggccc cttactgcat ttgaccaggg
      901 ctggggaacc atcagctatg ctgacatgtc gggccccagc gatgacaagc catactgctg
      961 gcactatccc ccacgaccgt gcggagtggt gccagcccaa agcgtctgtg gcccagtcta
     1021 ctgttttaca cccagcccag tggtcgtcgg caccactgac cgcagagggt atcccactta
     1081 caactggggc agcaatgaga ctgacgtcat tctcttgaac agcacgaggc cccctgctgg
     1141 cagttggttt ggctgtactt ggatgaacgc cacagggttt gtgaagactt gcggagcccc
     1201 gccctgcaac ttagggccga caggcaacac cagccttaag tgtcctactg attgcttccg
     1261 gaagcaccca gaagccacct acactaggtg cgggtcaggg ccctggctca ccccccggtg
     1321 tttggtggat tacccttaca ggttgtggca ttatccgtgc accgtaaact ataccatctt
     1381 caaggtgcgc atgtacattg gaggcttgga gcacaggctc gatgcagctt gcaactggac
     1441 ccgcggtgag cgatgtgatc tcgaagacag agatagggcc gagctgagcc cgctcctgca
     1501 caccaccaca cagtgggcca tattgccgtg ctctttcaca cccacgcctg ctcttagcac
     1561 tggcctcata cacttacatc aaaatatagt agacacccag tatctttacg gcctgagctc
     1621 cagcatcatc tcgtgggccg ttaagtggga gtatgtagta ctggcctttt tactactcgc
     1681 tgatgcccgt atctgtacct gcctatggat catgctccta gtttgccagg ctgaagcata
     1741 aaagccgaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
     1801 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     1861 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     1921 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     1981 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2041 tctatggctt ctgaggcgga aagaaccagt ggcggtaata cggttatcca cagaatcagg
     2101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2581 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2881 ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg ctatggcagg
     2941 gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg
     3001 tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac
     3061 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt
     3121 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     3181 tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg
     3241 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     3301 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     3361 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     3421 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     3481 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     3541 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     3601 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
     3661 agttcggctg gcgcgagccc ctgatgctct tgatcatcct gatcgacaag accggcttcc
     3721 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     3781 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     3841 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     3901 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     3961 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     4021 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     4081 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     4141 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     4201 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     4261 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     4321 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     4381 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     4441 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     4501 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     4561 ttcctttttc aataaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4621 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4681 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4741 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4801 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4861 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4921 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4981 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5041 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5101 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5161 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5221 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5281 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5341 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5401 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5461 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5521 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5581 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     5641 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5701 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5761 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5821 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5881 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5941 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6001 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     6061 ttacgcgcgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     6121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     6181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     6241 acgccaatag ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac
     6301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     6361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     6421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     6481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     6541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     6601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     6661 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
     6721 gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga tccactagtc
     6781 cagtgtggtg gaattcggct t
//
