LOCUS       sequence-180302-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated 
            insertion. Y205A mutation inhibits PKA-C kinase activity. R626A 
            should decrease affinity for cAMP without affecting specifity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Hackley CR, Mazzoni EO, Blau J
  TITLE     cAMPr: A single-wavelength fluorescent sensor for cyclic AMP.
  JOURNAL   Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. doi: 
            10.1126/scisignal.aah3738.
  PUBMED    29511120
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2018-03-6"; volume: "11(520). pii: 11/520/eaah3738.
            doi"; pages: " 10.1126/scisignal.aah3738"
COMMENT     Sequence Label: pUASTattb-cAMPr Y205A, R626A
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..95
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        114..352
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     299..320
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     1668..1689
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          2768..2833
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2963..2983
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3255..3389
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3292..3311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3346..3365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3418..3487
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3706..3724)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3745..3761)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3745..3761)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3758..3780)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3769..3785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3793..3799,3800..3817,3818..3823))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3838..3859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3976..3993)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4147..4735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4227..4246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4906..5697,5698..5766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5529..5548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5767..5871)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5897..6352)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6034..6055)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6246..6265
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6479..6501
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6493..6510
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6494..6510
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6517..6536
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6517..6535
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6569..6587)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7039..7110,7512..7785,7860..8514,8576..8891,9095..9226,
                     9297..9911)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    10686..10719
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
       61 agtactgtcc tccgagcgga gtactgtcct ccgagcggag actctagcga gcgccggagt
      121 ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac
      181 gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca
      241 gtaaagtgca agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc
      301 aactactgaa atctgccaag aagtaattat tgaatacaag aagagaactc tgaataggga
      361 attgggaatt cgttaacaga tctgcggccg cggctcgaca aaatgggcaa cgccgccgcc
      421 gccaagaagg gcagcgagca ggagagcgtg aaagagttcc tagccaaagc caaggaagat
      481 ttcctgaaaa aatgggagac cccttctcag aatacagccc agttggatca gtttgataga
      541 atcaagaccc ttggcaccgg ctcctttggg cgagtgatgc tggtgaagca caaggagagt
      601 gggaaccact acgccatgaa gatcttagac aagcagaagg tggtgaagct aaagcagatc
      661 gagcacactc tgaatgagaa gcgcatcctg caggccgtca acttcccgtt cctggtcaaa
      721 cttgaattct ccttcaagga caactcaaac ctgtacatgg tcatggagta tgtagctggt
      781 ggcgagatgt tctcccacct acggcggatt ggaaggttca gcgagcccca tgcccgtttc
      841 tacgcggcgc agatcgtcct gacctttgag tatctgcact ccctggacct catctaccgg
      901 gacctgaagc ccgagaatct tctcatcgac cagcagggct atattcaggt gacagacttc
      961 ggttttgcca agcgtgtgaa aggccgtact tggaccttgt gtgggacccc tgaggccttg
     1021 gcccccgaga ttatcctgag caaaggctac aacaaggctg tggactggtg ggctctcgga
     1081 gtcctcatct acgagatggc tgctggttac ccacccttct tcgctgacca gcctatccag
     1141 atctatgaga aaatcgtctc tgggaaggtg cggttcccat cccacttcag ctctgacttg
     1201 aaggacctgc tgcggaacct tctgcaagtg gatctcacca agcgctttgg gaacctcaag
     1261 aacggggtca atgacatcaa gaaccacaag tggtttgcca cgactgactg gattgccatc
     1321 tatcagagaa aggtggaagc tcccttcata ccaaagttta aaggccctgg ggacacgagt
     1381 aactttgacg actatgagga ggaagagatc cgggtctcca tcaatgagaa gtgtggcaag
     1441 gagtttactg agtttcccgg gaacgtctat atcaaggccg acgagcagaa gaacggcatc
     1501 aaggcgtact tcaagatccg ccacaacatc gagggcggcg gcgtgcagct cgcctaccac
     1561 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1621 agcgtgcagt ccatgctttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1681 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     1741 ggagggagcg aatccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1801 caggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggtgag
     1861 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1921 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1981 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacatccag
     2041 gagcgcacca tcttcttcaa gggcgacggc aactacaaga cccgcgccga ggtgaagttc
     2101 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2161 aacatcctgg ggcacaagct ggagtacaac accgcccccc ccgcatgcaa gggccggcgg
     2221 cgacgcgggg ccatcagcgc tgaggtctac accgaggagg atgccgcgtc ctatgttgct
     2281 aaggttatac cgaaagatta taagacaatg gctgctttag ctaaagccat tgaaaagaac
     2341 gtactgtttt cacatcttga tgataacgag agaagtgaca tttttgacgc catgttcccg
     2401 gtttccttta ttgctggaga gactgttatt cagcagggtg acgaagggga taacttctac
     2461 gtgattgacc aaggagagat ggacgtctat gtcaacaatg aatgggcaac cagtgttggg
     2521 gaaggaggga gcttcgggga gcttgctctg atttacggga ctcctcgagc ggccactgtc
     2581 aaggccaaga cgaacgtgaa actgtggggc attgaccggg acagctacag aaggatcctc
     2641 atgggaagca cgctgagaaa gcggaagatg taatcgaggg tacctctaga ggatctttgt
     2701 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     2761 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     2821 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     2881 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     2941 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     3001 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     3061 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     3121 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     3181 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3241 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3301 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3361 tcatcaatgt atcttatcat gtctggatcc actagtgtcg acgatgtagg tcacggtctc
     3421 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
     3481 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
     3541 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
     3601 gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac
     3661 ggtcacggcg ggcatgtcga cactagttct agccagcttt tgttcccttt agtgagggtt
     3721 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3781 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3841 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3901 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3961 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4321 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4561 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5881 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     5941 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     6001 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     6061 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     6121 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     6181 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     6241 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     6301 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     6361 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     6421 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     6481 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     6541 ttgggtacgt accgggcccc tagtatgtat gtaagttaat aaaacccatt tttgcggaaa
     6601 gtagataaaa aaaacatttt ttttttttac tgcactggat atcattgaac ttatctgatc
     6661 agttttaaat ttacttcgat ccaagggtat ttgatgtacc aggttctttc gattacctct
     6721 cactcaaaat gacattccac tcaaagtcag cgctgtttgc ctccttctct gtccacagaa
     6781 atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta
     6841 cgtagcgtca atgtccgcct tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa
     6901 gcggtttacg ccatcaatta aacacaaagt gctgtgccaa aactcctctc gcttcttatt
     6961 tttgtttgtt ttttgagtga ttggggtggt gattggtttt gggtgggtaa gcaggggaaa
     7021 gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc tattaattcg cggaggcagc
     7081 aaacacccat ctgccgagca tctgaacaat gtgagtagta catgtgcata catcttaagt
     7141 tcacttgatc tataggaact gcgattgcaa catcaaattg tctgcggcgt gagaactgcg
     7201 acccacaaaa atcccaaacc gcaattgcac aaacaaatag tgacacgaaa cagattattc
     7261 tggtagctgt tctcgctata taagacaatt tttgagatca tatcatgatc aagacatcta
     7321 aaggcattca ttttcgacta tattcttttt tacaaaaaat ataacaacca gatattttaa
     7381 gctgatccta gatgcacaaa aaataaataa aagtataaac ctacttcgta ggatacttcg
     7441 gggtactttt tgttcggggt tagatgagca taacgcttgt agttgatatt tgagatcccc
     7501 tatcattgca gggtgacagc ggagcggctt cgcagagctg cattaaccag ggcttcgggc
     7561 aggccaaaaa ctacggcacg ctccggccac ccagtccgcc ggaggactcc ggttcaggga
     7621 gcggccaact agccgagaac ctcacctatg cctggcacaa tatggacatc tttggggcgg
     7681 tcaatcagcc gggctccgga tggcggcagc tggtcaaccg gacacgcgga ctattctgca
     7741 acgagcgaca cataccggcg cccaggaaac atttgctcaa gaacggtgag tttctattcg
     7801 cagtcggctg atctgtgtga aatcttaata aagggtccaa ttaccaattt gaaactcagt
     7861 ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa
     7921 gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag ggcatccaag tatcgccatc
     7981 cgggatgcga ctgctcaatg gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc
     8041 ctatgtccag caggatgacc tctttatcgg ctccctaacg gccagggaac acctgatttt
     8101 ccaagccatg gtgcggatgc cacgacatct gacctatcgg cagcgagtgg cccgcgtgga
     8161 tcaggtgatc caggagcttt cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg
     8221 cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg gcattcgcct ccgaggctct
     8281 aaccgatccg ccgcttctga tctgcgatga gcccacctcc ggactggact cctttaccgc
     8341 ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac
     8401 cattcatcag ccgtcttccg agctgtttga gctctttgac aagatccttc tgatggccga
     8461 gggcagggta gctttcttgg gcactcccag cgaagccgtc gacttctttt cctagtgagt
     8521 tcgatgtgtt tattaagggt atctagtatt acataacatc tcaactccta tccagcgtgg
     8581 gtgcccagtg tcctaccaac tacaatccgg cggactttta cgtacaggtg ttggccgttg
     8641 tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa gatatgcgac aattttgcca
     8701 ttagcaaagt agcccgggat atggagcagt tgttggccac caaaaatctg gagaagccac
     8761 tggagcagcc ggagaatggg tacacctaca aggccacctg gttcatgcag ttccgggcgg
     8821 tcctgtggcg atcctggctg tcggtgctca aggaaccact cctcgtaaaa gtgcgactta
     8881 ttcagacaac ggtgagtggt tccagtggaa acaaatgata taacgcttac aattcttgga
     8941 aacaaattcg ctagatttta gatagaattg cctgattcca cacccttctt agtttttttc
     9001 aatgagatgt atagtttata gttttgcaga agataaataa atttcattta actcgcgaat
     9061 attaatgaga tgcgagtaac attttaattt gcagatggtt gccatcttga ttggcctcat
     9121 ctttttgggc caacaactca cgcaagtggg tgtgatgaat atcaacggag ccatcttcct
     9181 cttcctgacc aacatgacct ttcaaaacgt ctttgccacg ataaatgtaa gtcatgttta
     9241 gaatacattt gcatttcaat aatttactaa ctttctaatg aatcgattcg atttaggtgt
     9301 tcacctcaga gctgccagtt tttatgaggg aggcccgaag tcgactttat cgctgtgaca
     9361 catactttct gggcaaaacg attgccgaat tgccgctttt tctcacagtg ccactggtct
     9421 tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac ttcttcaact
     9481 gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat ctaatatcct
     9541 gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc ataccattcc
     9601 tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt
     9661 cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg
     9721 tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg
     9781 tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg
     9841 ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac
     9901 gcaaggagta gccgacatat atccgaaata actgcttgtt tttttttttt accattatta
     9961 ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat
    10021 aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag
    10081 actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact
    10141 cgtttatcgc tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg
    10201 cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc
    10261 ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta
    10321 gttccgcgcg attcggttcg ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa
    10381 ttagtaataa atatttgtat gtacaattta tttgctccaa tatatttgta tatatttccc
    10441 tcacagctat atttattcta atttaatatt atgacttttt aaggtaattt tttgtgacct
    10501 gttcggagtg attagcgtta caatttgaac tgaaagtgac atccagtgtt tgttccttgt
    10561 gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg tttatatata tcaaaaataa
    10621 caactataat aataagaata catttaattt agaaaatgct tggatttcac tggaactagg
    10681 ctagcataac ttcgtataat gtatgctata cgaagttatg ctagcggatc caagcttgca
    10741 tgcctgcagg t
//
