LOCUS       sequence-180303-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated 
            insertion. Y205A mutation inhibits PKA-C kinase activity. R739A 
            should increase affinity for cAMP without affecting specificity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Hackley CR, Mazzoni EO, Blau J
  TITLE     cAMPr: A single-wavelength fluorescent sensor for cyclic AMP.
  JOURNAL   Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. doi: 
            10.1126/scisignal.aah3738.
  PUBMED    29511120
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2018-03-6"; volume: "11(520). pii: 11/520/eaah3738.
            doi"; pages: " 10.1126/scisignal.aah3738"
COMMENT     Sequence Label: pUASTattb-cAMPr Y205A, R739A
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    24..118
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        137..375
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     322..343
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     1691..1712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          2791..2856
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2986..3006
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3278..3412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3315..3334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3369..3388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3441..3510
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3729..3747)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3768..3784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3768..3784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3781..3803)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3792..3808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3816..3822,3823..3840,3841..3846))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3861..3882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3999..4016)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4170..4758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4250..4269)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4929..5720,5721..5789))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5552..5571
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5790..5894)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5920..6375)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6057..6078)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6269..6288
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6502..6524
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6516..6533
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6517..6533
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6540..6559
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6540..6558
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6592..6610)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7062..7133,7535..7808,7883..8537,8599..8914,9118..9249,
                     9320..9934)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    10709..10742
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tccaagcttg catgcctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
       61 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      121 gagactctag cgagcgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
      181 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
      241 ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac
      301 cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc aagaagtaat tattgaatac
      361 aagaagagaa ctctgaatag ggaattggga attcgttaac agatctgcgg ccgcggctcg
      421 acaaaatggg caacgccgcc gccgccaaga agggcagcga gcaggagagc gtgaaagagt
      481 tcctagccaa agccaaggaa gatttcctga aaaaatggga gaccccttct cagaatacag
      541 cccagttgga tcagtttgat agaatcaaga cccttggcac cggctccttt gggcgagtga
      601 tgctggtgaa gcacaaggag agtgggaacc actacgccat gaagatctta gacaagcaga
      661 aggtggtgaa gctaaagcag atcgagcaca ctctgaatga gaagcgcatc ctgcaggccg
      721 tcaacttccc gttcctggtc aaacttgaat tctccttcaa ggacaactca aacctgtaca
      781 tggtcatgga gtatgtagct ggtggcgaga tgttctccca cctacggcgg attggaaggt
      841 tcagcgagcc ccatgcccgt ttctacgcgg cgcagatcgt cctgaccttt gagtatctgc
      901 actccctgga cctcatctac cgggacctga agcccgagaa tcttctcatc gaccagcagg
      961 gctatattca ggtgacagac ttcggttttg ccaagcgtgt gaaaggccgt acttggacct
     1021 tgtgtgggac ccctgaggcc ttggcccccg agattatcct gagcaaaggc tacaacaagg
     1081 ctgtggactg gtgggctctc ggagtcctca tctacgagat ggctgctggt tacccaccct
     1141 tcttcgctga ccagcctatc cagatctatg agaaaatcgt ctctgggaag gtgcggttcc
     1201 catcccactt cagctctgac ttgaaggacc tgctgcggaa ccttctgcaa gtggatctca
     1261 ccaagcgctt tgggaacctc aagaacgggg tcaatgacat caagaaccac aagtggtttg
     1321 ccacgactga ctggattgcc atctatcaga gaaaggtgga agctcccttc ataccaaagt
     1381 ttaaaggccc tggggacacg agtaactttg acgactatga ggaggaagag atccgggtct
     1441 ccatcaatga gaagtgtggc aaggagttta ctgagtttcc cgggaacgtc tatatcaagg
     1501 ccgacgagca gaagaacggc atcaaggcgt acttcaagat ccgccacaac atcgagggcg
     1561 gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1621 tgctgcccga caaccactac ctgagcgtgc agtccatgct ttcgaaagac cccaacgaga
     1681 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1741 acgagctgta caagggcggt accggaggga gcgaatccat ggtgagcaag ggcgaggagc
     1801 tgttcaccgg ggtggtgccc atccaggtcg agctggacgg cgacgtaaac ggccacaagt
     1861 tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1921 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1981 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     2041 ccatgcccga aggctacatc caggagcgca ccatcttctt caagggcgac ggcaactaca
     2101 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     2161 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacaccgccc
     2221 cccccgcatg caagggccgg cggcgacgcg gggccatcag cgctgaggtc tacaccgagg
     2281 aggatgccgc gtcctatgtt cggaaggtta taccgaaaga ttataagaca atggctgctt
     2341 tagctaaagc cattgaaaag aacgtactgt tttcacatct tgatgataac gagagaagtg
     2401 acatttttga cgccatgttc ccggtttcct ttattgctgg agagactgtt attcagcagg
     2461 gtgacgaagg ggataacttc tacgtgattg accaaggaga gatggacgtc tatgtcaaca
     2521 atgaatgggc aaccagtgtt ggggaaggag ggagcttcgg ggagcttgct ctgatttacg
     2581 ggactcctcg agcggccact gtcaaggcca agacgaacgt gaaactgtgg ggcattgacg
     2641 ctgacagcta cagaaggatc ctcatgggaa gcacgctgag aaagcggaag atgtaatcga
     2701 gggtacctct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     2761 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     2821 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     2881 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     2941 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     3001 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     3061 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     3121 ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag agatcataat
     3181 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3241 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     3301 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3361 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccactagtg
     3421 tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc
     3481 tccccgggcg cgtactccac ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg
     3541 cggtagttga tcccggcgaa cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg
     3601 ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg
     3661 ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt cgacactagt tctagccagc
     3721 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
     3781 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3841 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3901 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3961 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4861 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4921 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4981 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5041 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5101 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5161 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5221 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5281 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5341 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5401 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5461 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5521 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5581 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5641 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5701 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5761 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5821 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5881 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     5941 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6001 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6061 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6121 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6181 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6241 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6301 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6361 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6421 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     6481 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
     6541 aatacgactc actatagggc gaattgggta cgtaccgggc ccctagtatg tatgtaagtt
     6601 aataaaaccc atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg
     6661 gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt
     6721 accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt
     6781 tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc
     6841 tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg cattttgtca
     6901 gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc
     6961 caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt
     7021 tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg
     7081 agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta
     7141 gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg caacatcaaa
     7201 ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaattg cacaaacaaa
     7261 tagtgacacg aaacagatta ttctggtagc tgttctcgct atataagaca atttttgaga
     7321 tcatatcatg atcaagacat ctaaaggcat tcattttcga ctatattctt ttttacaaaa
     7381 aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata
     7441 aacctacttc gtaggatact tcggggtact ttttgttcgg ggttagatga gcataacgct
     7501 tgtagttgat atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag
     7561 ctgcattaac cagggcttcg ggcaggccaa aaactacggc acgctccggc cacccagtcc
     7621 gccggaggac tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca
     7681 caatatggac atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa
     7741 ccggacacgc ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct
     7801 caagaacggt gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc
     7861 caattaccaa tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg
     7921 atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg
     7981 cagggcatcc aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc
     8041 aaggagatgc aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta
     8101 acggccaggg aacacctgat tttccaagcc atggtgcgga tgccacgaca tctgacctat
     8161 cggcagcgag tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag
     8221 cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt
     8281 ctggcattcg cctccgaggc tctaaccgat ccgccgcttc tgatctgcga tgagcccacc
     8341 tccggactgg actcctttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag
     8401 aagggcaaga ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt
     8461 gacaagatcc ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc
     8521 gtcgacttct tttcctagtg agttcgatgt gtttattaag ggtatctagt attacataac
     8581 atctcaactc ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt
     8641 ttacgtacag gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc
     8701 caagatatgc gacaattttg ccattagcaa agtagcccgg gatatggagc agttgttggc
     8761 caccaaaaat ctggagaagc cactggagca gccggagaat gggtacacct acaaggccac
     8821 ctggttcatg cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc
     8881 actcctcgta aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg
     8941 atataacgct tacaattctt ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt
     9001 ccacaccctt cttagttttt ttcaatgaga tgtatagttt atagttttgc agaagataaa
     9061 taaatttcat ttaactcgcg aatattaatg agatgcgagt aacattttaa tttgcagatg
     9121 gttgccatct tgattggcct catctttttg ggccaacaac tcacgcaagt gggtgtgatg
     9181 aatatcaacg gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc
     9241 acgataaatg taagtcatgt ttagaataca tttgcatttc aataatttac taactttcta
     9301 atgaatcgat tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg
     9361 aagtcgactt tatcgctgtg acacatactt tctgggcaaa acgattgccg aattgccgct
     9421 ttttctcaca gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc
     9481 cggagtgctg cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac
     9541 gtccttcgga tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg
     9601 tccgccggtt atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt
     9661 gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct
     9721 gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac
     9781 cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct
     9841 gccgctggac tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct
     9901 ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt
     9961 gttttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct
    10021 aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc
    10081 ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac
    10141 ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt
    10201 atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt
    10261 cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga
    10321 cgaccttgga gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt
    10381 ggttcatttc gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc
    10441 caatatattt gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt
    10501 tttaaggtaa ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt
    10561 gacatccagt gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg
    10621 ttgtttatat atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat
    10681 gcttggattt cactggaact aggctagcat aacttcgtat aatgtatgct atacgaagtt
    10741 atgctagcgg a
//
