LOCUS       sequence-180307-       10679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide 
            targeted to Nuclei.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, 
            Mullineaux PM
  TITLE     Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei 
            provides a high-light signalling mechanism.
  JOURNAL   Nat Commun. 2017 Jun 29;8(1):49. doi: 10.1038/s41467-017-00074-w.
  PUBMED    28663550
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-29"; volume: "8(1):49. doi"; pages: " 
            10.1038/s41467-017-00074-w"
COMMENT     Sequence Label: pUB-nHyPer2 (NLS:HyPer2:NLS)
FEATURES             Location/Qualifiers
     source          1..10679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1338..1967
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2396..3469
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3535..3729
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3965..3987)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4073..4213
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4128..4147
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4228..4245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4399..4987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4479..4498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5233..6024)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6545..6569
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6662..6914)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6934..7485)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7527..7706)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    8128..8152
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8172..8192)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9624..9644)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(9668..9692)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10383..10399)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10383..10399)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10396..10418)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10407..10423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10431..10437,10438..10455,10456..10461))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10476..10497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(10662..10679,1..7)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
       61 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
      121 aagtacttta aagtacttta aagtacttta aagtactttg atccaacccc tccgctgcta
      181 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
      241 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
      301 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
      361 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
      421 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
      481 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
      541 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
      601 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
      661 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
      721 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
      781 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
      841 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
      901 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
      961 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     1021 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     1081 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     1141 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     1201 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     1261 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     1321 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     1381 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     1441 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     1501 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     1561 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     1621 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     1681 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     1741 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     1801 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     1861 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     1921 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     1981 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     2041 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     2101 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     2161 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     2221 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     2281 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     2341 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     2401 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     2461 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     2521 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     2581 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     2641 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     2701 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     2761 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     2821 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     2881 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     2941 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     3001 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     3061 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     3121 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     3181 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     3241 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     3301 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     3361 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     3421 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     3481 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     3541 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     3601 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     3661 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     3721 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     3781 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     3841 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     3901 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     3961 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4021 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     4081 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     4141 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4201 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4801 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5041 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
     5101 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
     5161 ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt
     5221 tctagctaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
     5281 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
     5341 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     5401 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     5461 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     5521 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     5581 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     5641 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
     5701 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     5761 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
     5821 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
     5881 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     5941 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
     6001 ttcggcgatc accgcttccc ccatgatgtt taactttgtt ttagggcgac tgccctgctg
     6061 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
     6121 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
     6181 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     6241 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
     6301 ccgaattgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
     6361 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
     6421 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
     6481 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     6541 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     6601 tgcggacgtt tttaatgtac tgaattaacg ccgaattgaa ttatcagctt gcatgccggt
     6661 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     6721 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     6781 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     6841 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     6901 atgtttgaac gatctgcttg actctagggg tcatcagatt tcggtgacgg gcaggaccgg
     6961 acggggcggc accggcaggc tgaagtccag ctgccagaaa cccacgtcat gccagttccc
     7021 gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg cctcgtgcat
     7081 gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga agccctgtgc
     7141 ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc gctggtggcg
     7201 gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg
     7261 acccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc agggatagcg
     7321 ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa
     7381 gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc
     7441 ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtag atcccctcga
     7501 tcgagttgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
     7561 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
     7621 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
     7681 cagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
     7741 tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatcg ataattcgcg
     7801 gtacccgggg atcctctaga gggcccgacg tcgcatgcct gcaggtcact ggattttggt
     7861 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     7921 tttcttatat gctcaacaca tgagcgaaac cctataagaa ccctaattcc cttatctggg
     7981 aactactcac acattattct ggagaaaaat agagagagat agatttgtag agagagactg
     8041 gtgatttttg cggactctag catggccgct tataaactag ttggatatct cgagtcgaca
     8101 cgcgtagatc tccgcggacg tcccatcacc actttgtaca agaaagctgg gtcggcgcgc
     8161 ccaccctttc aaacctttct cttcttctta ggcactgctt gcttcaaaac cttatcaaag
     8221 tgaccatcca tcctggctct tatagcctct gctaactgtt cataccttga tctcaatggt
     8281 gaacctggcc tgtagaccaa tccaatggtc cttcttggct ctggtttaat gcatggaaga
     8341 taaactacac catccctctt cctctctggt ggcacggcaa gtgctggtaa caaagttatt
     8401 ccacttccaa cggctaccat gttcctcaag gtttctagtg atgtagccct gaagtgagta
     8461 tcctcgtcag caccggcttc gaagcaaaat ccggttccgt tgtactctag cttgtgtcca
     8521 agaatatttc cgtcctcttt gaatccgata cctttaagct ctattctatt caccaaagta
     8581 tctccctcaa acttcacttc tgccctagtt ttataattac cgtcgtcttt gaagaatata
     8641 gtcctctctt gcacatatcc ctcaggcata gcactcttga agaagtcatg ttgcttcata
     8701 tggtcaggat atcttgcgaa gcactttaaa ccgtaaccta aggttgttac taatgttggc
     8761 catgggacag gaagttttcc agttgtacag attaatttca atgttagttt tccgtaggtt
     8821 gcgtcaccct caccttctcc ggagactgaa aatttgtgac cgttcacgtc accatcaagt
     8881 tctactaata ttgggacaac tcctgtgaac aactcttctc ccttggaacc tgtacctcca
     8941 ctacctccgt ctacattgta tagctcatcc ataccaagag taataccggc agcggttacg
     9001 aactcaagca acaccatgtg atcccttttc tcatttgggt ctttggatag gacagactga
     9061 aaagataggt agtggttatc tggaagtaaa acaggtccgt caccgattgg ggtgttctgt
     9121 tgataatggt ctgctagttg tacggaacca tcttccacgt tatgtctaat tttgaagttg
     9181 gcctttatac cattcttctg tttgtcggcc atgatataga cgttatctga attgtaaccg
     9241 gcggacatag cttgatccct caagcagtgt ccatcctcaa gcattagaag tttctcaccg
     9301 gccaaatctg ccattggaac gcattctcta ttggcccatg ggtgatcttc atagattgct
     9361 aaaagcatag gttcatcgaa aagaggcact tctatgaatg cctcggattc ctttaccaaa
     9421 gctagtatga cacaatcaag tttaccacta tctaactggg ccaatagttg atgagtctgt
     9481 gcctcgtgaa ggtacatttc aagtttaggg aaagtttggt gtagcattgg gataatgtga
     9541 ggaagtaagt aaggtccaac tgttgggatt agaccgatat gcaatggtcc tgacatagtc
     9601 tcaccctgtt gagaagccat ttcaaccttt ctcttcttct taggcatggt gaagggggcg
     9661 gccgcggagc ctgctttttt gtacaaactt gtgatgggcc ctctagagga tcccgcactc
     9721 gagtgttaat cagaaaaact cagattaatc tacaaattcg atcgcacaaa ctagaaacta
     9781 acaccagatc tagatagaaa tcacaaatcg aagagtaatt attcgacaaa actcaaatta
     9841 tttgaacaaa tcggatgata tctatgaaac cctaatcgag aattaagatg atatctaacg
     9901 atcaaaccca gaaaatcgtc ttcgatctaa gattaacaga atctaaacca aagaacatat
     9961 acgaaattgg gatcgaacga aaacaaaatc gaagattttg agagaataag gaacacagaa
    10021 atttaccttg atcacggtag agagaattga gagaaagttt ttaagatttt gagaaattga
    10081 aatctgaatt gtgaagaaga agagctcttt gggtattgtt ttatagaaga agaagaagaa
    10141 aagacgagga cgactaggtc acgagaaagc taaggcggtg aagcaatagc taataataaa
    10201 atgacacgtg tattgagcgt tgtttacacg caaagttgtt tttggctaat tgccttattt
    10261 ttaggttgag gaaaagtatt tgtgctttga gttgataaac acgactcgtg tgtgccggct
    10321 gcaaccactt tgacgccgtt tattactgac tcgtcgggta ccgagctcga attcgtaatc
    10381 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    10441 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    10501 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10561 atcggccaac gcgcggggag aggcggtttg cgtattggct agagcagctt gagcttggat
    10621 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcg
//
