LOCUS       sequence-18031-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  TITLE     Shivdasani lab plasmids
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p079 pPNT6
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(57..90)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(148..171)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     545..564
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gcggtggcgg ccgcacgcgt cgacgcgttt aaacgaggta ccagtggcgc gccggcataa
       61 cttcgtatag catacattat acgaagttat aggcctgcag gtcaattcta ccgggtaggg
      121 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      181 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      241 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      301 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      361 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
      421 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
      481 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
      541 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
//
